Immune responses in mice vaccinated with a DNA vaccine expressing a new elastase from Trichinella spiralis

Title:

Immune responses in mice vaccinated with a DNA vaccine expressing a new elastase from Trichinella spiralis

Creator:

Xin Zhuo Zhang, Sun, Xiang Yuan, Bai, Ying, Yue, Wen Wen, Yue, Xin, Song, Yan Yan, Cui, Jing, and Wang, Zhong Quan

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:13e557b7-8c37-4421-ab0d-75b55407c902
uuid:13e557b7-8c37-4421-ab0d-75b55407c902
doi:10.14411/fp.2020.027

Subject:

Trichinella, trichinellosis, vaccination, and protective immunity

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

The elastase, which belongs to the serine protease family, hydrolyses various proteins and may be involved in the parasite invasion. In this study, complete sequence of elastase-1 (TsE) the nematode Trichinella spiralis (Owen, 1835) was cloned into the plasmid pcDNA3.1 as TsE DNA vaccine. After intramuscular vaccination, serum anti-Trichinella antibodies (IgG and subclass IgG1/IgG2a, and IgA), total and specific intestinal mucosal sIgA in mice vaccinated with pcDNA3.1/TsE were measured by ELISA. The results showed that vaccination with pcDNA3.1/TsE induced a systemic humoral immune response (high levels of serum IgG and subclass IgG1/IgG2a and IgA) and local intestinal mucosal immune responses (high levels of TsE-specific sIgA). Vaccination of mice with TsE DNA vaccine also triggered a systemic and local concomitant Th1/Th2 response, as demonstrated by significant elevation of Th1 (IFN-γ and IL-2) / Th2 (IL-4 and IL-10) cytokine levels after the spleen, mesenteric lymph node and Peyer's patch cells from vaccinated mice were stimulated with recombinant TsE (rTsE). The vaccination of mice with pcDNA3.1/TsE displayed a 17% reduction of intestinal adult worms and a 39% reduction of muscle larvae. Our results indicated that TsE DNA vaccine elicited a systemic concomitant Th1/Th2 response and an enteral local sIgA response, and produced a partial protection against infection with T. spiralis. The TsE may be regarded as a potential candidate vaccine target against Trichinella infection. The oral polyvalent vaccines should be developed to improve the protective efficacy of anti-Trichinella vaccines.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

1-11

Source:

Folia parasitologica | 2020 Volume:67 | Number:1

Harvested from:

CDK

Metadata only:

false