The interaction of osteoblasts with bone-implant materials: 1. the effect of physicochemical surface properties of implant materials

Title:

The interaction of osteoblasts with bone-implant materials: 1. the effect of physicochemical surface properties of implant materials

Creator:

Dana Kubies, Lucie Himmlová, Riedel, T., Chánová, E., Karel Balík, Douděrová, M., Jarmila Bártová, and Vlasta Pešáková

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:19e79a24-fffb-4767-b1a5-cc86ce454ad7
uuid:19e79a24-fffb-4767-b1a5-cc86ce454ad7

Subject:

Fyziologie člověka a srovnávací fyziologie, implantáty, cytokiny, implants, cytokines, surface properties, proliferation, cell number, osteoblast, surface free energy, wettability, blood clot formation, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

This comparative study of various surface treatments of commercially available implant materials is intended as guidance for orientation among particular surface treatment methods in term of the cell reaction of normal human osteoblasts and blood coagulation. The influence of physicochemical surface parameters such as roughness, surface free energy and wettability on the response of human osteoblasts in the immediate vicinity of implants and on the blood co agulation was studied. The osteoblast proliferation was monitored and the expression of tissue mediators (TNF-α , IL-8, MMP-1, bone alkaline phosphatase, VCAM-1, TGF-β ) was evaluated after the cell cultivation onto a wide range of commercially available materials (titanium and Ti6Al4V alloy with various surface treatments, CrCoMo alloy, zirconium oxide ceramics, polyethylene and carbon/carbon composite). The formation of a blood clot was investigated on the samples immersed in a freshly drawn whole rabbit blood using scanning electron microscope. The surfaces with an increased osteoblast proliferation exhibited particularly higher surface roughness (here Ra > 3.5 μm) followed by a high polar part of the surface free energy whereas the effect of wettability played a minor role. The surface roughness was also the main factor regulating the blood coagulation. The blood clot formation analys is showed a rapid coag ulum formation on the rough titanium-based surfaces. The titanium with an etching treatment was considered as th e most suitable candidate for healing into the bone tissue due to high osteoblast proliferation, the highest production of osteogenesis markers and low production of inflammatory cytokines and due to the most intensive blood clot formation., D. Kubies ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:1

Harvested from:

CDK

Metadata only:

false