Regulation of the ubiquitin proteasome system in mechanically injured human skeletal muscle

Title:

Regulation of the ubiquitin proteasome system in mechanically injured human skeletal muscle

Creator:

Seifert, M., Gosenca, D., Ponelies, N., Ising, N., Patel, M. B., Obertacke, U., and Majetschak, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:4d910937-c847-431b-848b-0d3686bdb371
uuid:4d910937-c847-431b-848b-0d3686bdb371
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, biochemie, katabolismus, biochemistry, catabolism, svalová kachexie, epoxomicin, muscle cachexia, ubiquitin-protein ligase complexes, epoxomixin, adamantane-acetyl-(6-aminohexanoyl)3-(leucinyl)3-vinyl(methyl)-sulfone, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Metabolic consequences of direct muscle trauma are insufficiently defined. Their effects on the ubiquitin-proteasome pathway (UPP) of protein degradation in human skeletal muscles are as yet unknown. Thus, we investigated whether the UPP is involved in the metabolic response evoked in directly traumatized human skeletal muscles. Biopsies were obtained from contused muscles after fractures and from normal muscles during elective implant removal (control). As estimated by western blot analyses, concentrations of free ubiquitin and ubiquitin protein conjugates were similar in extracts from injured and uninjured muscles. Ubiquitin protein ligation rates were reduced after injury (1.5±0.2 vs. 1.0±0.15 fkat/μg; p=0.04). Chymotryptic-, tryptic- and caspase-like proteasome peptidase activities (total activity minus activity in the presence of proteasome inhibitors) increased significantly after trauma (p=0.04 - 0.001). Significant increases in total chymotryptic- and caspase-like activities were attributable to proteasome activation. Our results extend the possible role of the UPP in muscle wasting to direct muscle trauma. They further suggest that the effects of direct mechanical trauma are not limited to the proteasome and imply that ubiquitin protein ligase systems are also involved. Based on the potential role of the UPP in systemic diseases, it might also be a therapeutic target to influence muscle loss in critically ill blunt trauma patients, in which large proportions of muscle are exposed to direct trauma. and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:2

Harvested from:

CDK

Metadata only:

false