Influence of Thyroid Status on the Differentiation of Slow and Fast Muscle Phenotypes

Title:

Influence of Thyroid Status on the Differentiation of Slow and Fast Muscle Phenotypes

Creator:

Vadászová, A., Zachařová, G., Macháčová, K., Jirmanová, I., and Soukup, T.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:5d53bffb-6229-47a6-be3e-449c9560bf54
uuid:5d53bffb-6229-47a6-be3e-449c9560bf54

Subject:

Muscle fiber types, Muscle innervation, Thyroid hormones, Muscle proteins, Myosin heavy chains, Muscle activity, Mitochondrial GPDH, and Calcium transporters

Type:

article, model:article, and TEXT

Description:

Muscle phenotype is determined by combined effects of intrinsic genetic and extrinsic factors like innervation, hormonal levels and mechanical factors or muscle activity. We have been studying the effect of altered thyroid hormone levels on the expression of myosin heavy chain (MyHC) isoforms in control and regenerating soleus and extensor digitorum longus muscles of euthyroid, hypothyroid or hyperthyroid female inbred Lewis rats. The fiber type composition has been determined according to the mATPase activity and immunocytochemical staining of MyHC isoforms, the content of MyHC isoforms has been determined by SDS-PAGE, the mRNA levels have been measured by RT-PCR and the ultrastructural transformation has been analyzed by electron-microscopy. Our results indicate that although the innervation plays a decisive role in the determination of muscle phenotype, levels of thyroid hormones contribute to the extent of muscle phenotype transformation.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2004 Volume:53 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false