Chromosomal composition of the genome in the monomorphic diplokaryotic microsporidium Paranosema grylli: analysis by two-dimensional pulsed-field gel electrophoresis

Title:

Chromosomal composition of the genome in the monomorphic diplokaryotic microsporidium Paranosema grylli: analysis by two-dimensional pulsed-field gel electrophoresis

Creator:

Nassonova, Elena, Cornillot, Emmanuel, Méténier, Guy, Agafonova, Nina, Kudryavtsev, Boris, Skarlato, Sergei, and Vivarès, Christian P.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:641ba558-b8d8-400d-bc7c-f247beb5bcda
uuid:641ba558-b8d8-400d-bc7c-f247beb5bcda
doi:10.14411/fp.2005.019

Subject:

Microsporidia, diplokaryon, molecular karyotype, KARD 2-D PFGE, chromosome-length polymorphism, ploidy, and meiosis

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The molecular karyotype of Paranosema grylli Sokolova, Seleznev, Dolgikh et Issi, 1994, a monomorphic diplokaryotic microsporidium, comprises numerous bright and faint bands of nonstoichiometric staining intensity. Restriction analysis of chromosomal DNAs by ''karyotype and restriction display'' 2-D PFGE has demonstrated that the complexity of molecular karyotype of P. grylli is related to the pronounced length polymorphism of homologous chromosomes. The background of this phenomenon is discussed in the context of ploidy state, reproductive strategy and population structure in this microsporidium. We propose that the remarkable size variation between homologous chromosomes in P. grylli may be a consequence of ectopic recombination at the chromosome extremities.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

145-157

Source:

Folia parasitologica | 2005 Volume:52 | Number:1-2

Harvested from:

CDK

Metadata only:

false