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Start Over Creator Méténier, Guy

1. Chromosomal composition of the genome in the monomorphic diplokaryotic microsporidium Paranosema grylli: analysis by two-dimensional pulsed-field gel electrophoresis

Creator:
Nassonova, Elena, Cornillot, Emmanuel, Méténier, Guy, Agafonova, Nina, Kudryavtsev, Boris, Skarlato, Sergei, and Vivarès, Christian P.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Microsporidia, diplokaryon, molecular karyotype, KARD 2-D PFGE, chromosome-length polymorphism, ploidy, and meiosis
Language:
English
Description:
The molecular karyotype of Paranosema grylli Sokolova, Seleznev, Dolgikh et Issi, 1994, a monomorphic diplokaryotic microsporidium, comprises numerous bright and faint bands of nonstoichiometric staining intensity. Restriction analysis of chromosomal DNAs by ''karyotype and restriction display'' 2-D PFGE has demonstrated that the complexity of molecular karyotype of P. grylli is related to the pronounced length polymorphism of homologous chromosomes. The background of this phenomenon is discussed in the context of ploidy state, reproductive strategy and population structure in this microsporidium. We propose that the remarkable size variation between homologous chromosomes in P. grylli may be a consequence of ectopic recombination at the chromosome extremities.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Post-genomics of microsporidia, with emphasis on a model of minimal eukaryotic proteome: a review

Creator:
Texier, Catherine, Brosson, Damien, El Alaoui, Hicham, Méténier, Guy, and Vivarès, Christian P.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Microsporidia, Encephalitozoon, intracellular parasite, post-genomics, protein size, deletions, and proteome analysis
Language:
English
Description:
The genome sequence of the microsporidian parasite Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923 contains about 2,000 genes that are representative of a non-redundant potential proteome composed of 1,909 protein chains. The purpose of this review is to relate some advances in the characterisation of this proteome through bioinformatics and experimental approaches. The reduced diversity of the set of E. cuniculi proteins is perceptible in all the compilations of predicted domains, orthologs, families and superfamilies, available in several public databases. The phyletic patterns of orthologs for seven eukaryotic organisms support an extensive gene loss in the fungal clade, with additional deletions in E. cuniculi. Most microsporidial orthologs are the smallest ones among eukaryotes, justifying an interest in the use of these compacted proteins to better discriminate between essential and non-essential regions. The three components of the E. cuniculi mRNA capping apparatus have been especially well characterized and the three-dimensional structure of the cap methyltransferase has been elucidated following the crystallisation of the microsporidial enzyme Ecm1. So far, our mass spectrometry-based analyses of the E. cuniculi spore proteome has led to the identification of about 170 proteins, one-quarter of these having no clearly predicted function. Immunocytochemical studies are in progress to determine the subcellular localisation of microsporidia-specific proteins. Posttranslational modifications such as phosphorylation and glycosylation are expected to be soon explored.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public