Characterization of the quenching of chlorophyll a fluorescence by β-carotene using the non-linear analysis

Title:

Characterization of the quenching of chlorophyll a fluorescence by β-carotene using the non-linear analysis

Creator:

Gazdaru, D. M. and Iorga, B.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:64c454df-1f34-449d-9764-cd08e4a4c256
uuid:64c454df-1f34-449d-9764-cd08e4a4c256
issn:0300-3604
doi:10.1023/A:1015672516537

Subject:

fluorescence quenching and photosynthesis

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Carotenoids (Car) regulate energy flow in photosynthesis by a specific Car-chlorophyll (Chl) interaction in the singlet-excited states, leading to a reduction in Chl fluorescence. We studied quenching of Chl a-fluorescence in benzene by trans-β-carotene. Non-linear analysis of the quenching process enables to explain the possible molecular mechanism leading to the de-excitation of Chl a. The fluorescence intensity was measured at 670 nm for excitation wavelengths of 380, 430, 640, and 650 nm. The β -carotene concentrations ranged from 4×10-5 M to 5×10-3 M. When the samples were excited at 640 and 650 nm, the Stern-Volmer plots showed that the quenching process has high rate constants, hence β-carotene is a very efficient quencher. Two different types of quenching process could take place. and D. M. Gazdaru, B. Iorga.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

607-609

Source:

Photosynthetica | 2001 Volume:39 | Number:4

Harvested from:

CDK

Metadata only:

false