1. Characterization of the quenching of chlorophyll a fluorescence by β-carotene using the non-linear analysis
- Creator:
- Gazdaru, D. M. and Iorga, B.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- fluorescence quenching and photosynthesis
- Language:
- Multiple languages
- Description:
- Carotenoids (Car) regulate energy flow in photosynthesis by a specific Car-chlorophyll (Chl) interaction in the singlet-excited states, leading to a reduction in Chl fluorescence. We studied quenching of Chl a-fluorescence in benzene by trans-β-carotene. Non-linear analysis of the quenching process enables to explain the possible molecular mechanism leading to the de-excitation of Chl a. The fluorescence intensity was measured at 670 nm for excitation wavelengths of 380, 430, 640, and 650 nm. The β -carotene concentrations ranged from 4×10-5 M to 5×10-3 M. When the samples were excited at 640 and 650 nm, the Stern-Volmer plots showed that the quenching process has high rate constants, hence β-carotene is a very efficient quencher. Two different types of quenching process could take place. and D. M. Gazdaru, B. Iorga.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public