Age-related susceptibility and resistance to nonlethal Plasmodium yoelii infection in C57BL/6 mice

Title:

Age-related susceptibility and resistance to nonlethal Plasmodium yoelii infection in C57BL/6 mice

Creator:

Shan, Ying, Liu, Ju, Jiang, Yong-Jun, Shang, Hong, Jiang, Dong, and Cao, Ya-Ming

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7626e04d-23de-4531-9ff3-6d383ed83a94
uuid:7626e04d-23de-4531-9ff3-6d383ed83a94
doi:10.14411/fp.2012.021

Subject:

Plasmodium yoelii 17XNL, age, immune response, dendritic cells, and Tregs

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

In cases of human malaria, children suffer very high rates of morbidity and mortality. To analyze the mechanisms involved in age-dependent protection against malaria, we investigated the characterization of immune responses to Plasmodium yoelii 17XNL (P.y 17XNL) in young (3 weeks) and middle-aged (8 months) C57BL/6 mice. In this study, we found that 100% of young mice succumbed to P.y 17XNL infection with higher parasitemia, while middle-aged mice were able to clear blood parasites and no mortality was observed. These observations suggested that the young C57BL/6 mice were susceptible to P.y 17XNL infection, whereas the middle-aged mice were resistant. Cellular analysis revealed that both the numbers of splenic myeloid dendritic cells (mDCs) as well as the expression of DC maturation markers were higher in middle-aged mice than those in young mice. The numbers of IgG1- or IgG2a-secreting B cells increased markedly in middle-aged mice after infection with P.y 17XNL. The dynamic change of the number of CD4+CD25+Foxp3+ regulatory T cells (Tregs) in mice infected with P.y 17XNL was also different between the two groups. In addition, the levels of IFN-γ and NO increased in both groups during early parasite infection, while there was also an obvious increase in IL-4 production in the infected middle-aged mice. The change in IL-10 levels following infection was consistent with that of the change in the number of Tregs. The survival of middle-aged mice following P.y 17XNL infection was dependent upon the establishment of effective Th1 and Th2 responses and a successful switch between Th1 and Th2 responses, as well as appropriate functioning of Tregs.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

153-161

Source:

Folia parasitologica | 2012 Volume:59 | Number:3

Harvested from:

CDK

Metadata only:

false