The karyotype of bream Abramis brama was analysed by means of C-banding, replication banding, DAPI fluorescent staining and in situ hybridisation with 18S rDNA and telomeric probes. The use of the in vivo 5-bromodeoxyuridine incorporation technique enabled the induction of replication bands of the RBA type in the karyotype. C-bands corresponded to late replicated chromosome regions. 18S rDNA clusters were found on one chromosome pair. Telomeric sequences were observed only on ends of chromatids. The karyotype morphology and NOR phenotype of A. brama are very similar to those found in other species of the leuciscine cyprinids karyotyped so far.