Diurnal variation of 6β-hydroxycortisol in cardiac patients

Title:

Diurnal variation of 6β-hydroxycortisol in cardiac patients

Creator:

Stanislav Mičuda, Luděk Šišpera, Martin Hodač, Fuksa, L., Eva Doleželová, Jaroslav Cerman, Jolana Cermanová, and Jiřina Martínková

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b6b0f87f-514e-49b7-8dd2-8465d55e71b7
uuid:b6b0f87f-514e-49b7-8dd2-8465d55e71b7
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, biochemie, kortizol, biochemistry, cortisol, CYP3A4, 6β-hydroxycortisol, diurnal variation, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The 24-hour urinary excretion of 6-β-hydroxycortisol (6β-OHC) and the urinary ratio of 6β-hydroxycortisol/cortisol (6β-OHC/UFC) have been proposed as noninvasive probes for human cytochrome P450 3A4 isoform (CYP3A4). In this study, we evaluated within- and between-day variability of 6β-OHC excretion and 6β-OHC/UFC ratio in nine Caucasian men with cardiac disease. Each study participant was asked to collect 24-hour urine specimens during four consecutive days in five standardized time intervals. Concentrations of UFC and 6β-OHC were determined by immunoassay and the high-performance liquid chromatographic (HPLC) method, respectively. The HPLC method was accurate and precise, as indicated by the recovery rate of 96.5-103.3 % and less than 5.2 % and 6.3 % of the coefficient of variation for within-run and between-run assay, respectively. In patients, diurnal variations in UFC and 6β-OHC excretion were parallel. Consequently, 6β-OHC/UFC ratio remained stable during the day. Both, 6β-OHC excretion and 6β-OHC/UFC ratio showed significant relationship between 24-hour value and values measured in corresponding collection periods with best correlations obtained from night interval (22.00-06.00, r = 0.86-0.91). These results indicated that urinary 6β-OHC excretion and 6β-OHC/UFC ratio measured in overnight/morning urine could precisely reflect 24-hour values even in severely ill patients. In addition, a simple and sensitive HPLC method was described for determination of 6β-OHC in urine., S. Mičuda, L. Šišpera, M. Hodač, P. Pařízek, L. Fuksa, E. Brčáková, J. Cerman, J. Cermanová, J. Martínková., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:3

Harvested from:

CDK

Metadata only:

false