Glycerophosphate-Dependent Hydrogen Peroxide Production by Rat Liver Mitochondria

Title:

Glycerophosphate-Dependent Hydrogen Peroxide Production by Rat Liver Mitochondria

Creator:

Ješina, P., Kholová, D., Bolehovská, R., Červinková, Z., Drahota, Z., and Houštěk, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c19de93d-6e86-445a-aba5-9950ae154672
uuid:c19de93d-6e86-445a-aba5-9950ae154672

Subject:

Rat liver mitochondria, Mitochondrial glycerophosphate dehydrogenase, Triiodothyronine, and Hydrogen peroxide

Type:

article, model:article, and TEXT

Description:

We studied the extent to which hormonally-induced mitochondrial glycerophosphate dehydrogenase (mGPDH) activity contributes to the supply of reducing equivalents to the mitochondrial respiratory chain in the rat liver. The activity of glycerophosphate oxidase was compared with those of NADH oxidase and/or succinate oxidase. It was found that triiodothyronine-activated mGPDH represents almost the same capacity for the saturation of the respiratory chain as Complex II. Furthermore, the increase of mGPDH activity induced by triiodothyronine correlated with an increase of capacity for glycerophosphate-dependent hydrogen peroxide production. As a result of hormonal treatment, a 3-fold increase in glycerophosphate-dependent hydrogen peroxide production by liver mitochondria was detected by polarographic and luminometric measurements.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2004 Volume:53 | Number:3

Harvested from:

CDK

Metadata only:

false