Search

Start Over Creator Drahota, Z.

1. Cold-activated growth of rat brown adipose tissue in the neonatal period

Creator:
Drahota, Z. and Hahn, P.
Type:
article, model:article, and TEXT
Subject:
brown adipose tissue, newborn rats, and cold stress
Language:
English
Description:
Repeated exposure of newborn rats to a short-term cold load between day 10 and 30 after birth stimulates the physiological growth rate of the brown adipose tissue. This indicates that in the neonatal period the brown adipose tissue has no reserve thermogenic capacity to compensate an additional cold load and therefore the rate of tissue growth must be activated.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Developmental aspects of lipid metabolism

Creator:
Koldovský, O., Dobiášová, M., Drahota, Z., and Hahn, P.
Type:
article, model:article, and TEXT
Subject:
energy sources, fat metabolism, cholesterol metabolism, white and brown adipose tissue, carnitine synthesis, and carnitine in milk
Language:
English
Description:
It was confirmed that the main source of energy for growth and development in the neonatal period was fat. Considerable attention was paid to the development of both white adipose tissue (WAT) and brown adipose tissue (BAT) in the rat and human newborn. Cholesterol metabolism during development was studied in the liver, the small intestine and both WAT and BAT. Brown adipose tissue of rats and adipose tissue from human newborns require carnitine for optimum respiration and fatty acid oxidation. Surprisingly, carnitine enhanced lipolysis in human newborn adipose tissue, Intravenously-fed newborn patients exhibited a rapid decrease of plasma level of carnitine and its esters, indicating a greater requirement for exogenous carnitine than in adult subjects (52 references)
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Developmental Changes of Cytochrome c Oxidase and Citrate Synthase in Rat Heart Homogenate

Creator:
Drahota, Z., Milerová, M., Stieglerová, A., Houštěk, J., and Ošťádal, B.
Type:
article, model:article, and TEXT
Subject:
Rat heart, Postnatal development, Cytochrome c oxidase, and Citrate synthase
Language:
English
Description:
Activity of cytochrome c oxidase and citrate synthase in rat heart homogenates was determined in 5-, 15-, 28- and 60-day-old rats. The activity of both enzymes increased during postnatal development but their changes followed different kinetics. The membrane-bound cytochrome c oxidase reached its adult values during the early postnatal period, i.e. between days 5 and 15, whereas soluble matrix-localized citrate synthase also continued to increase between days 15 and 60. Our data indicate a relative excess of cytochrome c oxidase in neonatal cardiocytes.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Enteral administration of lipid emulsions protects liver cytochrome c oxidase from hepatotoxic action of thioacetamide

Creator:
Červinková, Z. and Drahota, Z.
Type:
article, model:article, and TEXT
Subject:
cytochrome c oxidase, thioacetamide, lipids, and liver
Language:
English
Description:
During 48 hours after application sublethal doses of hepatotoxic agent thiacetamide decrease cytochrome c oxidase activity of rat liver homogenate and isolated mitochondria to 46 % and 32 % of original values, respectively. This decrease may be prevented by simultaneous application of lipid emulsion (mixture of Lipofundin and Mygliol) twice a day.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

5. Function of coenzyme Q in the cell: some biochemical and physiological properties

Creator:
Rauchová, H., Drahota, Z., and Lenaz, G.
Type:
article, model:article, and TEXT
Subject:
coenzyme Q, mitochondrial respiratory chain, inner cell membranes, antioxidant effect, and therapeutic effect
Language:
English
Description:
Coenzyme Q (CoQ), a lipophilic substituted benzoquinone, is well known as a redox component of the mitochondrial and many bacterial respiratory chains. However, additional locations and roles have been recently discovered. CoQ is described as a redox component of extramitochondrial electron transport chains and it is a powerful antioxidant and a membrane stabilizer. Increasing evidence for the beneficial clinical effects of CoQ administration in senescence or in different disorders (e.g. cerebrovascular, muscular, neurogenic) may be explained by the multiple roles of CoQ in cells.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

6. Glycerophosphate-Dependent Hydrogen Peroxide Production by Rat Liver Mitochondria

Creator:
Ješina, P., Kholová, D., Bolehovská, R., Červinková, Z., Drahota, Z., and Houštěk, J.
Type:
article, model:article, and TEXT
Subject:
Rat liver mitochondria, Mitochondrial glycerophosphate dehydrogenase, Triiodothyronine, and Hydrogen peroxide
Language:
English
Description:
We studied the extent to which hormonally-induced mitochondrial glycerophosphate dehydrogenase (mGPDH) activity contributes to the supply of reducing equivalents to the mitochondrial respiratory chain in the rat liver. The activity of glycerophosphate oxidase was compared with those of NADH oxidase and/or succinate oxidase. It was found that triiodothyronine-activated mGPDH represents almost the same capacity for the saturation of the respiratory chain as Complex II. Furthermore, the increase of mGPDH activity induced by triiodothyronine correlated with an increase of capacity for glycerophosphate-dependent hydrogen peroxide production. As a result of hormonal treatment, a 3-fold increase in glycerophosphate-dependent hydrogen peroxide production by liver mitochondria was detected by polarographic and luminometric measurements.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

7. Lipid peroxidation in isolated membranes of cerebral cortex, heart and kidney

Creator:
Rauchová, H., Kalous, M., Drahota, Z., Koudelová, J., and Mourek, J.
Type:
article, model:article, and TEXT
Subject:
ADP.Fe/NADPH-induced lipid peroxidation, cerebral cortex, heart, kidney, and NADPH cytochrome c reductase
Language:
English
Description:
The extent of ADP.Fe/NADPH-induced lipid peroxidation measured as production of thiobarbituric acid-reactive substances (TBARS) was determined in isolated membranes from cerebral cortex, heart and kidney of 21-days- old rats. The time course of lipid peroxidation showed higher production of TBARS in cerebral cortex than in heart and kidney. Our data indicate that high level of TBARS production is not due to high activity of NADPH oxidoreductase but due to high content of endogenous lipids in cerebral cortex membranes that could be modified. Higher production of TBARS in cerebral cortex is the result of higher content of lipids in cerebral cortex membranes because NADPH cytochrome c reductase activity in membranes of cerebral cortex is lower than that of heart and kidney.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

8. Modification of calcium retention capacity of rat liver mitochondria by phosphate and tert-butyl hydroperoxide

Creator:
Endlicher, R. , Drahota, Z., and Červinková, Z.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
liver mitochondria, calcium retention capacity, mitochondrial permeability transition pore, phosphate, and tert-butyl hydroperoxide
Language:
English
Description:
By determining the calcium retention capacity (CRC) of rat liver mitochondria, we confirmed and extended previous observations describing the activation of mitochondrial swelling by phosphate and tert-butyl hydroperoxide (t-BHP). Using CRC measurements, we showed that both phosphate and t-BHP decrease the extent of calcium accumulation required for the full mitochondrial permeability transition pore (MPTP) opening to 35 % of control values and to only 15 % when both phosphate and t-BHP are present in the medium. When changes in fluorescence were evaluated at higher resolution, we observed that in the presence of cyclosporine A fluorescence values return after each Ca2+ addition to basal values obtained before the Ca2+ addition. This indicates that the MPTP remains closed. However, in the absence of cyclosporine A, the basal fluorescence after each Ca2+ addition continuously increased. This increase was potentiated both by phosphate and t-BHP until the moment when the concentration of intramitochondrial calcium required for the full opening of the MPTP was reached. We conclude that in the absence of cyclosporine A, the MPTP is slowly opened after each Ca2+ addition and that this rate of opening can be modified by various factors such as the composition of the media and the experimental protocol used.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

9. Neutron-capture therapy of brain tumours: neutron sources, neutron-capture drugs, biological tests and clinical perspectives in the Czech Republic

Creator:
Burian, J., Marek, M., Mareš, V., and Drahota, Z.
Type:
article, model:article, and TEXT
Subject:
epithermal neutrons, sodium borocaptate, neutron-capture therapy, and brain tumours
Language:
English
Description:
The paper reviews neutron sources, chemical compounds and clinical perspectives of the boron neutron-capture therapy of brain tumours. Special attention is paid to the physical characteristics and biological effectiveness of the epithermal neutron beam constructed at the LVR-15 nuclear reactor at Řež near Prague.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

10. Protective effect of carnitine on lipoperoxide formation in rat brain

Creator:
Koudelová, J., Mourek, J., Drahota, Z., and Rauchová, H.
Type:
article, model:article, and TEXT
Subject:
brain (rat), hypobaric hypoxia, lipid peroxidation, and carnitine
Language:
English
Description:
Carnitine administration (by intraperitoneal injection) to 21-day-old-rats prevents the increase of thiobarbituric acid-reactive substances (index of lipid peroxidation and free radical damage) induced by 30 min hypobaric hypoxia in four different parts of the brain (cerebral cortex, subcortical structures, medulla oblongata and cerebellum).
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public