In vitro secretion of metabolic end-products by piscine haemoflagellates Cryptobia salmositica and C. bullocki (Kinetoplastida: Bodonidae) and the relationship of these products to the pH in the medium

Title:

In vitro secretion of metabolic end-products by piscine haemoflagellates Cryptobia salmositica and C. bullocki (Kinetoplastida: Bodonidae) and the relationship of these products to the pH in the medium

Creator:

Ardelli, Bernadette F. and Woo, Patrick T.K.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ebafb61f-0d41-4a53-a55a-f06a955e75c2
uuid:ebafb61f-0d41-4a53-a55a-f06a955e75c2
doi:10.14411/fp.2001.031

Subject:

Cryptobia, pyruvate, lactate, hydrogen peroxide, and pH

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Pathogenic and nonpathogenic strains of Cryptobia salmositica Katz, 1951 and C. bullocki Strout, 1965 produced hydrogen peroxide, pyruvate and lactate under in vitro conditions in Minimum Essential Medium (MEM). As parasite number increased, the phenol red in the medium changed from red to yellow. This change was not associated with a decrease in pH, or an increase in pyruvate or lactate, but was correlated with an increased secretion of hydrogen peroxide. Parasites incubated at 10°C in medium at pH 6.0, 6.5, 7.0 and 7.3 were active for about 1 week with decreasing activity in the absence of serum. Parasites in saline (pH 6.0, 6.5, 7.0 and 7.3) were nonmotile within 24 h and were dead in about 1 week. This suggests that these Cryptobia spp. are sensitive to changes in pH and require medium which is buffered, either with serum or Hepes.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

187-191

Source:

Folia parasitologica | 2001 Volume:48 | Number:3

Harvested from:

CDK

Metadata only:

false