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1. In vitro secretion of metabolic end-products by piscine haemoflagellates Cryptobia salmositica and C. bullocki (Kinetoplastida: Bodonidae) and the relationship of these products to the pH in the medium

Creator:
Ardelli, Bernadette F. and Woo, Patrick T.K.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Cryptobia, pyruvate, lactate, hydrogen peroxide, and pH
Language:
English
Description:
Pathogenic and nonpathogenic strains of Cryptobia salmositica Katz, 1951 and C. bullocki Strout, 1965 produced hydrogen peroxide, pyruvate and lactate under in vitro conditions in Minimum Essential Medium (MEM). As parasite number increased, the phenol red in the medium changed from red to yellow. This change was not associated with a decrease in pH, or an increase in pyruvate or lactate, but was correlated with an increased secretion of hydrogen peroxide. Parasites incubated at 10°C in medium at pH 6.0, 6.5, 7.0 and 7.3 were active for about 1 week with decreasing activity in the absence of serum. Parasites in saline (pH 6.0, 6.5, 7.0 and 7.3) were nonmotile within 24 h and were dead in about 1 week. This suggests that these Cryptobia spp. are sensitive to changes in pH and require medium which is buffered, either with serum or Hepes.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Utilization of photosynthates during rubber biosynthesis in guayule (Parthenium argentatum Cray)

Creator:
Ramachandra Reddy, A. and Rama Das, V.S.
Format:
print
Type:
model:internalpart and TEXT
Subject:
enolase, 3- phosphoglycerate, ubber transferase, pyruvate kinase, pyruvate dehydrogenase, pyruvate, phosphoglyceromutase, phosphoenolpyruvate, isopentenyl pyrophosphate isomerase, and isopentenyl
Language:
Multiple languages
Description:
Incorporation of labelled CO2, 3-phosphoglycerate (PGA), phosphoenolpyruvate (PEP) and pyruvate into hexane extractable rabber ffactions in the cut shoots of guayule {Parthenium argentatum Gray) was determined in order to evaluate the role of photosynthesis in providing precursors for rubber biosynthesis. DCMU inhibited the incorporation of labelled CO2 and PGA into rubber. The incorporation of i'*C02 into rubber depended on irradiance. Enzymatic activities of phosphoglyceromutase, enolase, pyruvate kinase and pyruvate dehydrogenase complex found in purified chloroplasts from the leaves indicated the chloroplast autonomy for intraplastid acetyl coenzyme A formation. The enzymes related to the biosynthesis of isopentenyl pyrophosphate (IPP) were associated with both leaf and stem extracts. Rubber producing enzyme activities, námely IPP isomerase and rubber transferase, were abundantly localized in roots and stems of guayule while the leaves exhibited low activities of these enzymes. Hence the leaves of guayule play a major role in providing precursors for rubber formation in stems and roots.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public