The Application of the Leibniz’s Dialogical Method in the Correspondence with Clarke In the annotated Czech translation of Leibniz’s dialogue Pacidius Philalethi, which was published in 2019, but also in an earlier essay by the same translator on Leibniz’s dialogic way of writing as well as in other interpretations, there is little discussion of the practical implications of his dialogic method. Leibniz’s dialogical argumentation strategy, as he later applied it in his correspondence with Samuel Clarke, is therefore either completely neglected or is referred to as being typical of the time, or alternatively is confused with Zeno’s dialectical method. Thus, the aim of this study is to clarify the practical implications of Leibniz’s application of the dialogic method in his final correspondence with Clarke. and V komentovaném českém překladu Leibnizova dialogu Pacidius Philalethi, který vyšel v roce 2019, jakož i v dřívějším pojednání téhož překladatele o Leibnizově dialogickém způsobu psaní, ale i v dalších výkladech se o praktických důsledcích Leibnizovy dialogické metody lze dočíst jen málo. Leibnizova dialogická argumentační strategie, jak ji posléze aplikoval ještě i při korespondenci s Clarkem, tak zůstává buď zcela opomenuta, nebo bývá označována za dobově typickou, případně zaměňována s dialektickou metodou Zénónovou. Cílem této studie je proto objasnit praktické důsledky Leibnizovy aplikace dialogické metody při jeho závěrečné korespondenci s Clarkem.
Small subunit rRNA sequences were obtained from 38 representatives mainly of the nematode orders Spirurida (Camallanidae, Cystidicolidae, Daniconematidae, Philometridae, Physalopteridae, Rhabdochonidae, Skrjabillanidae) and, in part, Ascaridida (Anisakidae, Cucullanidae, Quimperiidae). The examined nematodes are predominantly parasites of fishes. Their analyses provided well-supported trees allowing the study of phylogenetic relationships among some spirurine nematodes. The present results support the placement of Cucullanidae at the base of the suborder Spirurina and, based on the position of the genus Philonema (subfamily Philoneminae) forming a sister group to Skrjabillanidae (thus Philoneminae should be elevated to Philonemidae), the paraphyly of the Philometridae. Comparison of a large number of sequences of representatives of the latter family supports the paraphyly of the genera Philometra, Philometroides and Dentiphilometra. The validity of the newly included genera Afrophilometra and Caranginema is not supported. These results indicate geographical isolation has not been the cause of speciation in this parasite group and no coevolution with fish hosts is apparent. On the contrary, the group of South-American species of Alinema, Nilonema and Rumai is placed in an independent branch, thus markedly separated from other family members. Molecular data indicate that the skrjabillanid subfamily Esocineminae (represented by Esocinema bohemicum) should be either elevated to the rank of an independent family or Daniconematidae (Mexiconema africanum) should be decreased to Daniconematinae and transferred to the family Skrjabillanidae. Camallanid genera Camallanus and Procamallanus, as well as the subgenera Procamallanus and Spirocamallanus are confirmed to be paraphyletic. Paraphyly has also been found within Filarioidea, Habronematoidea and Thelazioidea and in Cystidicolidae, Physalopteridae and Thelaziidae. The results of the analyses also show that Neoascarophis, Spinitectus and Rhabdochona are monophyletic, in contrast to the paraphyletic genus Ascarophis. They further confirm the independence of two subgenera, Rhabdochona and Globochona, in the genus Rhabdochona. The necessity of further studies of fish-parasitizing representatives of additional nematode families not yet studied by molecular methods, such as Guyanemidae, Lucionematidae or Tetanonematidae, is underscored.