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Start Over Creator Mak, Joon Wah

1. Electrophoretic variations of peptidase E (PEPE) in characterizing clones and isolates of Plasmodium falciparum from different geographical areas

Creator:
Ang, Hooi Hoon, Chan, Kit Lam, and Mak, Joon Wah
Format:
print
Type:
model:internalpart and TEXT
Subject:
clones, electrophoretic variations, isolates, and peptidase E
Language:
English
Description:
Six clones were obtained from each Plasmodium falciparum (Welch, 1897) isolate from different geographical areas, Gombak A (Malaysian), Gombak С (Malaysian), ST 9 (Malaysian), ST 12 (Malaysian), ST 85 (Malaysian), ST 148 (Malaysian), Gambian (African) and TGR (Thailand) isolates using the limiting dilution method (Rosario 1981). Forty-eight clones were obtained and were characterized by an electrophoresis isoenzyme analysis of PEPE (Peptidase E) (EC. 3.4.11 or 13). Results showed that they were pure clones as they were monovariant with regards to this enzyme unlike their parent isolates which were divariant.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. In vitro susceptibility studies of Malaysian Plasmodium falciparum isolates and clones against schizontocidal drugs

Creator:
Ang, Hooi Hoon, Chan, Kit Lam, and Mak, Joon Wah
Format:
print
Type:
model:internalpart and TEXT
Subject:
Schizontocidal drugs, heterogenous isolates, homogeneous clones, and Plasmodium falciparum.
Language:
English
Description:
Five Malaysian isolates of the protozoan Plasmodium falciparum Welch were cultured in vitro following the method of Träger and Jensen (1976, 1977) and subsequently cloned using the limiting dilution method of Rosario (1981). Thirty clones were obtained and were later characterized against schizontocidal drugs, chloroquinc, mefloquine and quinine, using the modified in vitro microtechnique. Results showed that these local isolates were heterogenous and most of the clones exhibited similar pattern of susceptibility as their parent isolate except for ST 168 clone and two ST 195 clones that were sensitive but two ST 165 clones, two ST 168 clones and five ST 195 clones were resistant against quinine, respectively. Results also indicated that they were pure clones compared to their parent isolate because their drug susceptibility studies were significantly different (p < 0.05).
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public