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1. Electrophoretic variations of peptidase E (PEPE) in characterizing clones and isolates of Plasmodium falciparum from different geographical areas

Creator:
Ang, Hooi Hoon, Chan, Kit Lam, and Mak, Joon Wah
Format:
print
Type:
model:internalpart and TEXT
Subject:
clones, electrophoretic variations, isolates, and peptidase E
Language:
English
Description:
Six clones were obtained from each Plasmodium falciparum (Welch, 1897) isolate from different geographical areas, Gombak A (Malaysian), Gombak С (Malaysian), ST 9 (Malaysian), ST 12 (Malaysian), ST 85 (Malaysian), ST 148 (Malaysian), Gambian (African) and TGR (Thailand) isolates using the limiting dilution method (Rosario 1981). Forty-eight clones were obtained and were characterized by an electrophoresis isoenzyme analysis of PEPE (Peptidase E) (EC. 3.4.11 or 13). Results showed that they were pure clones as they were monovariant with regards to this enzyme unlike their parent isolates which were divariant.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Physiological and ultrastructural responses of sour orange (Citrus aurantium L.) clones to water stress

Creator:
Ben Salem-Fnayou, A. , Belghith, I., Lamine, M., Mliki, A., and Ghorbel, A.
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
biomasa, chloroplasty, zavlažování, biomass, chloroplasts, ultrastruktura chloroplastu, výměna plynu, sucho, klony, mezibuněčná koncentrace CO2, list, chloroplast ultrastructure, gas exchange, drought, avoidance strategy, Cleopâtra mandarin, clones, indol-butyric acid, irrigation, intercellular CO2 concentration, leaf, mesophyll, 2, and 581
Language:
Multiple languages
Description:
Water stress is a major abiotic constraint leading to serious crop losses. Recently, in the Mediterranean region, water stress has become markedly sensed, especially in Citrus orchards. This study investigated the physiological responses of local sour orange (Citrus aurantium L.) clones to severe water stress. Water stress was applied by withholding irrigation during weeks, followed by a rewatering phase during three months. Under water stress, sour orange clones decreased their stomatal conductance, net photosynthetic rate, and transpiration rate. On the contrary, biomass was stable, especially in the Kliaa clone. In addition, reduced leaf water potentials (-3 MPa) and water contents were measured in most of the clones, except Kliaa which kept the highest water potential (-2.5 MPa). After rewatering, all clones recovered except of the Ghars Mrad (GM) clone. Ultrastructural observations of leaf sections by transmission electron microscopy did not reveal marked alterations in the mesophyll cells and chloroplast structure of Kliaa in comparison to the sensitive clone GM, in which palisade parenchyma cells and chloroplasts were disorganized. This contrasting behavior was mainly attributed to genetic differences as attested by molecular analysis. This study highlighted GM as the drought-sensitive clone and Kliaa as the tolerant clone able to develop an avoidance strategy based on an efficient stomatal regulation. Although a high percentage of polyembryony characterizes C. aurantium and justifies its multiplication by seeds, heterogeneous water-stress responses could be observed within sour orange plants in young orchards., A. Ben Salem-Fnayou, I. Belghith, M. Lamine, A. Mliki, A. Ghorbel., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public