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2. Differences in haemolytic action of Hg2+ in relation to its concentration and ionic strength of incubating solutions

Creator:
Mojžiš, J. and Nicák, A.
Type:
article, model:article, and TEXT
Subject:
erythrocytes, HgCl2, haemolysis, and ionic strength
Language:
English
Description:
The haemolytic action of different concentrations of HgCl2 on rat red blood cells (RBC ) was studied in vitro. The concentrations of HgCb in incubating media were 0.15, 0.25 and 0.50 mmol.l'1. The ionic strength of the media varied from 0 to 154 mmol.l1 NaCl, Isotonicity of solutions was also compensated using isotonic glucose in different concentrations (287-0 mmol.l"1). Osmolarity of solutions varied from 287 to 308 mOsm. Besides these solutions the haemolysis in Krebs-Ringer solution was also studied. Haemolysis was characterized with two maxima in all concentrations of Hg2+. The first maximum was observed at low ionic strength and the second one at high ionic strength. In relation to the increased concentrations of Hg2+, the first maximum of haemolysis progressively declined towards the higher ionic strength. In the Krebs-Ringer solutions, the increased concentration of Hg2+ was followed by reduced haemolysis. The haemolytic concentration of 0.15 mmol.l"1 was found to be optimal.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Effect of allopurinol and superoxide dismutase on indomethacin-induced gastric lesions in the rat

Creator:
Kohút, A. and Mojžiš, J.
Type:
article, model:article, and TEXT
Subject:
gastric lesions, gastric vascular permeability, gastric emptying, allopurinol, and superoxide dismutase
Language:
English
Description:
Gastric lesions induced by indomethacin (20 mg.kg'1 i.p.) were studied in rats after a 24 hour fast. The size of the lesions was correlated with gastric vascular permeability (determined from the Evans blue concentration in the stomach tissue after its i.v administration) and with the rate of gastric emptying (determined from the phenol red concentration after its intragastric application). These changes were correlated with the prevention of gastric lesions by allopurinol (50 mg.kg'1) after a single dose or once daily for 3 days before indomethacin and by a single dose (15 000 dkg'1) of superoxide dismutase (SOD). Indomethacin significantly increases the rate of gastric emptying concomitantly with gastric vascular permeability. The pretreatment of animals with allopurinol and SOD inhibits gastric lesions as well as gastric vascular permeability without changing gastric emptying which was increased after indomethacin administration. The inhibition of gastric lesion formation and gastric vascular permeability was more marked in rats pretreated with allopurinol for 3 days when compared with rats treated with a single dose of allopurinol only. These results support the suggestion that oxygen-derived free radicals contribute to the pathogenesis of indomethacin-induced gastric lesions.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Effect of stobadine on gastric mucosal injury after ischaemia / reperfusion

Creator:
Mojžiš, J., Pomfy, M., Kohút, A., Beneš, L., Nicák, A., and Mirossay, L.
Type:
article, model:article, and TEXT
Subject:
stobadine, ischaemia / reperfusion, free radicals, and gastric mucosal injury
Language:
English
Description:
The ability of stobadine to prevent gastric mucosal injury was tested in rat gastric ischaemia induced by 30 min clamping of the coeliac artery with subsequent 30 min reperfusion. Serious injury of gastric mucosa (macroscopic and microscopic) and the increase of microvascular permeability was found after ischaemia/reperfusion in rats without stobadine. After oral pretreatment with stobadine (5 mg.kg-1, 30 min before surgery), the development of gastric mucosal lesions and changes of vascular permeability were significantly decreased.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

5. Gastroprotective effect of malotilate in indomethacin- and ethanol-induced gastric mucosal damage

Creator:
Mirossay, L., Mojžiš, J., Šallingová, Z., Bodnar, J., Benický, M., Böör, A., and Kohút, A.
Type:
article, model:article, and TEXT
Subject:
damage, ethanol, gastric, indomethacin, malotilate, and gastroprotection
Language:
English
Description:
Malotilate as a synthetic substance shares comparable hepatoprotective properties with various flavonoids. The gastroprotective effect of some flavonoids prompted us to ascertain the similar effectiveness of malotilate. The possible gastroprotectivity was examined in gastric mucosal damage in rats induced by indomethacin (20 mg.kg-1) or ethanol (96 %). Oral pretreatment with malotilate (25, 50, 100, 200 and 400 mg.kg-1) reduced the extent of lesions induced by both indomethacin and ethanol. Histological analyses also revealed a mitigating effect on the severity of gastric mucosal lesions. Similar results were obtained in the group of rats pretreated with 5 mg.kg-1 indomethacin followed by oral administration of 96 % ethanol. This finding suggests that the effect of malotilate on rat gastric mucosa is independent of endogenous prostaglandin production.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

6. The influence of glucose and saccharose on haemolytic action of HgCl2 in relation to the ionic strength of the incubating medium

Creator:
Nicák, A. and Mojžiš, J.
Type:
article, model:article, and TEXT
Subject:
erythrocytes, haemolysis, HgCl2, glucose, saccharose, and ionic strength
Language:
English
Description:
The haemolytic action of HgCl2 (0, 15 mmol.l'1) was studied in relation to the ionic strength and concentration of glucose and saccharose in incubating medium. Blood from 94 donors, aged 19-46 years were used in our experiments. In relation to the ionic strength the haemolytic action was characteristic with two maxima of haemolysis. The first at low ionic strength and second one at the high. Both maxima in solutions containing saccharose were significantly diminished in glucose. These facts show a negative influence of saccharose on the haemorheological properties of the erythrocyte membrane.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public