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Start Over Creator Rudenko, Nataliia

3. Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls

Creator:
Šikutová, Silvie, Rudolf, Ivo, Golovchenko, Maryna, Rudenko, Nataliia, Grubhoffer, Libor, and Hubálek, Zdeněk
Format:
bez média and svazek
Type:
model:article and TEXT
Language:
English
Description:
A total of 150 nymphal Ixodes ricinus (L., 1758) (Acari: Ixodidae) from the Czech Republic were examined for Anaplasma phagocytophilum (Foggie, 1949) Dumler et al., 2001 by PCR using EHR521/747 primers: 22 of 50 pools were positive (minimum prevalence, 14.7%). However, sequencing of the PCR products did not show complete homology with A. phagocytophilum (91%) while the closest relationship (95%) was found to "Candidatus Ehrlichia walkerii". The results indicate a need for care in interpretation of Anaplasma PCR results and for PCR optimization for detecting A. phagocytophilum in ticks.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Lyme borreliosis: insights into tick- / host-borrelia relations

Creator:
Grubhoffer, Libor, Golovchenko, Maryna, Vancová, Marie, Zacharovová-Slavíčková, Klára, Rudenko, Nataliia, and Oliver Jr., James H.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
borrelia spirochete, Lyme borreliosis, ticks, vectors, interactions, and glycoproteins
Language:
English
Description:
Lyme borreliosis (LB) is a serious infectious disease of humans and some domestic animals in temperate regions of the Northern Hemisphere. It is caused by certain spirochetes in the Borrelia burgdorferi sensu lato (s.l.) species complex. The complex consists of 11 species (genospecies). Borrelia burgdorferi sensu stricto (s.s.), Borrelia garinii and Borrelia afzelii are the major agents of human disease. Borrelia burgdorferi s.l. species are transmitted mainly by ticks belonging to the Ixodes ricinus species complex plus a few additional species not currently assigned to the complex. B. burgdorferi infections may produce an acute or chronic disease with a wide array of clinical symptoms such as erythema migrans (EM), carditis, arthritis, neuroborreliosis, and acrodermatitis chronica atrophicans (ACA). Differences in LB spirochetes ''genospecies'' and strains/isolates determine the occurrence and severity of this multi-system disease. Accurate and reliable identification of the LB spirochetes in ticks as well as knowledge of their prevalence are essential for prevention against the disease and development of an effective vaccine. An overview of the knowledge of molecular factors with emphasis on potential protein-carbohydrate interactions in the tick-borrelia system is the main focus of this review.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public