Electric stimulation (ES) could induce contraction of intestinal smooth muscle. The aim of this study was to analyze the effects of ES on esophageal motility and the underlying mechanism in vivo. Twenty-eight rabbits were equipped with a pair of subserosa electrodes (connected to an electrical stimulator) in the lower segment of the esophagus. The ES signal consisted of bipolar rectangular pulse trains, lasting for 3 s, with different amplitudes (1 mA, 3 mA, 5 mA and 10 mA), and frequencies (10 Hz, 20 Hz and 50 Hz). The amplitude of the contraction was recognized by high-resolution manometry. The effect of ES was tested under anesthesia and following administration of atropine, phentolamine or L-NAME. ES induced esophageal contraction at the stimulated site. A statistically significant increase in esophageal pressure was observed when the stimulation amplitude was above 3 mA. The increase in esophageal pressure was associated with the amplitude of stimulus as well as the frequency. During stimulation, atropine, phentolamine and L-NAME had no effect on the increase of esophageal pressure induced by ES. These findings implied that ES induced esophageal contraction were not mediated via the NANC, adrenergic or cholinergic pathway. The amplitude of esophageal contraction was current and frequency dependent., L. Zhang, W. Zhao, C. Zhao, H. Jin, B. Wang, B. Wang., and Seznam literatury
Using the FLAC3D numerical simulation method, the characteristics of mining stress evolution, fault activation patterns, and fault energy evolution characteristics are simulated and analyzed in the process of the footwall and hanging wall working face heading to a normal fault. The study shows that the fault cut off the mining stress propagation of overlying strata, and the stress blocking effect due to the fault is evident. When working faces head towards a fault, the abutment pressure and the vertical stress of the surrounding rock increase first and subsequently decrease. The abutment pressure of the coal wall and fault is highest when the distances to the fault are 40 m and 30 m for the footwall and hanging wall working faces, respectively. Moreover, the hanging wall mining stress is higher than the footwall mining stress. Fault activation in high-located strata precedes that in low-located strata when working faces head towards the normal fault. Energy is gradually accumulated before unstable fault events take place because of mining, and fault instability quickly releases a lot of energy. The mining stress concentration and fault activation due to faults easily result in rock bursts and mine seismicity, Wu Quan-sen, Jiang Li-shuai and Wu Quan-lin., and Obsahuje bibliografické odkazy
Harrisovská transformační gramatika a z ní vycházející francouzská škola Maurice Grosse a Gastona Grosse zavedly pojem tzv. verbe support (zhruba odpovídající pojmu kategoriálního slovesa) pro slovesa vyskytující se ve verbonominálních konstrukcích. Tato slovesa nejsou sama o sobé predikáty, nýbrž pouze aktualizují nominální predikát. Syntakticko-sémantická povaha daného predikativního substantiva určuje výbér příslušného kategoriálního slovesa. Článek ukazuje, že vzhledem к tomu, že v italštině se zdá být tvoření těchto predikativních substantiv (deverbálních a denominálních) produktivním procesem, je možné pokusit se navrhnout některá syntakticko-sémantická kritéria výběru příslušného kategoriálního slovesa. Tyto syntakticko-sémantické podmínky jsou hypoteticky formulovány pro dvě běžná a základní kategoriální slovesa: fare a dare.
Our study aimed to investigate subacute exposure to alcohol in relation to bone microstructure of mice. Animals from experimental (E) group drank a solution composed of 15 % ethanol and water for 14 days (one remodeling cycle), while those from control (C) group drank only water. In the compact bone of E group, decreased bone formation and increased porosity were observed which corresponds with lower levels of serum alkaline phosphatase and glutathione. Alcohol significantly increased sizes of primary osteon's vascular canals and decreased those of secondary osteons, Haversian canals. Relative bone volume, bone mineral density (BMD), relative bone volume without marrow cavity were also lower in E group. On the contrary, trabecular bone microstructure did not differ significantly between E and C groups. Liver function test showed higher levels of alanine aminotransferase, aspartate aminotransferase in alcohol-fed mice. Serum calcium, phosphate were significantly lower in E group. According to our study, only changes in compact bone microstructure of mice following one remodeling cycle were observed due to both direct and indirect effects of alcohol., A. Sarocka, V. Kovacova, R. Omelka, M. Bauerova, E. Kapusta, Z. Goc, G. Formicki, M. Martiniakova., and Obsahuje bibliografii
Agonist-induced subcellular redistribution of G-protein coupled receptors (GPCR) and of trimeric guanine-nucleotide binding regulatory proteins (G-proteins) represent mechanisms of desensitization of hormone response, which have been studied in our laboratory since 1989. This review brings a short summary of these results and also presents information about related literature data covering at least small part of research carried out in this area. We have also mentioned sodium plus potassium dependent adenosine triphosp hatase (Na, K-ATPase) and 3H-ouabain binding as useful reference standard of plasma membrane purity in the brain., Z. Drastichová, L. Bouřová, V. Lisý, L. Hejnová, V. Rudajev, J. Stöhr, D. Durchánková, P. Ostašov, J. Teisinger, T. Soukup, J. Novotný, P. Svoboda., and Obsahuje bibliografii a bibliografické odkazy
The variations in antibody responses (total IgG and IgGl, IgG2a, IgG2b, and IgG2c subclasses) were studied in two groups of rats infected with metacercariae of the trematode Fasciola hepatica L. Animals of group 1 were 4 weeks old, and rats of group 2 were 13 weeks old. All IgG subclasses increased during the course of infection except IgG2c, which decreased. The younger rats reached more marked responses than the older, at least during the period of this trial. IgGl and IgG2a antibodies reached the highest levels, and among these two, IgG2a response was slightly superior to IgGl,