Data mining animal of genomes has been used before to identify endoparasites, and may be a particularly useful tool to surpass some difficulties faced by studies in the marine environment. We detected a species of Sarcocystis Lankester, 1882, contamination in the sperm whale (Physeter catodon Linnaeus) reference genome available in the GenBank database. We identified and extracted multiple gene fragments and placed the sequences in a phylogenetic framework. Our results indicate that the sequences of Sarcocystis sp. found in the genome do not correspond to any currently described species, despite a few other similar sequences having been identified in fur seals (Pinnipedia) and another sperm whale. Including data from previous studies, we suggest there is enough evidence to support the occurrence of at least four species of Sarcocystis in marine mammals. We also demonstrate that the term "S. canis-like" has been used for samples not closely related to Sarcocystis canis Dubey et Speer, 1991.
Determination of malondialdehyde is a widely used procedure for measurement of lipid peroxidation. In this paper we report an unusual temperature dependence of malondialdehyde formation in egg yolk phosphatidylcholine liposomes oxidized by the Fenton system (0.1 mmol/1 FeSC>4 and 0.05 mmol/1 H2O2). The amount of malondialdehyde formed was 37 % higher in samples kept at 22 °C than at 50 °C. An alternative method for determination of lipid peroxidation, measurement of oxygen uptake, revealed complete consumption of dissolved oxygen to peroxidized lipids at 22 °C as well as 50 °C. Since oxygen is essential for the formation of cyclic peroxides - precursors of malondialdehyde - we conclude that the nature of the observed effect consists in limitation of oxygen availability at elevated temperatures.
The hormone leptin, which is thought to be primarily produced by adipose tissue, is a polypeptide that was initially characterized by its ability to regulate food intake and energy metabolism. Leptin appears to signal the status of body energy stores to the brain, resulting in the regulation of food intake and whole-body energy expenditure. Subsequently, it was recognized as a cytokine with a wide range of peripheral actions and is involved in the regulation of a number of physiological systems including reproduction. In the fed state, leptin circulates in the plasma in proportion to body adiposity in all species studied to date. However other factors such as sex, age, body mass index (BMI), sex steroids and pregnancy may also affect leptin levels in plasma. In pregnant mice and humans, the placenta is also a major site of leptin expression. Leptin circulates in biological fluids both as free protein and in a form that is bound to the soluble isoform of its receptor or other binding proteins such as one of the immunoglobulin superfamily members Siglec-6 (OBBP1). Although the actions of leptin in the control of reproductive function are thought to be exerted mainly via the hypothalamicpituitary-gonadal axis, there have also been reports of local direct effects of leptin at the peripheral level, however, these data appear contradictory. Therefore, there is a need to summarize the current status of research outcomes and analyze the possible reasons for differing results and thus provide researchers with new insight in designing experiments to investigate leptin effect on reproduction. Most importantly, our recent experimental data suggesting that reproductive performance is improved by decreasing concentrations of peripheral leptin was unexpected and cannot be explained by hypotheses drawn from the experiments of excessive exogenous leptin administration to normal animals or ob/ob mice., M. Herrid, S. K. A. Palanisamy, U. A. Ciller, R. Fan, P. Moens, N. A. Smart, J. R. McFarlane., and Obsahuje bibliografii
The goal of the present study is to understand the discoursive negotiation of the claims upon the use of public space. Specifically is to focused on the analysis of purification of public space from homeless persons in middle-sized city. On the basis of my research of homeless persons that included de semi-structured interviews with selected actors (policemen, politicians, employees of social services and of the non-profit organizations), analysis of documents (especially journalistic and legal) and active participant observation.
Vibration of engineering structures results in time variable displacements functions. For realistic structures modes of vibration are determined by exciting frequency and the natural frequencies of the structure. Hence, the components of stress tensor are time function too. In the presented paper the general idea of method of cycle-counting based on the FFT analysis for engineering structures is discussed and analytically verified for the case vibrating beam excited by series pulse-type force with no damping. and V názvu článku je jazyková chyba/překlep - analitical - správně = analytical
The formulations and interpretations of some formally analogous expressions of the single-particle scalar quantum wave equations of electrons and photons, propagated in adequate isotropic static media under weak signals and interaction phenomena, are presented in this article. and V prezentovaném článku jsou obsaženy formulace a interpretace některých formálně analogických tvarů jednočásticových skalárních kvantových vlnových rovnic šířících se elektronů a fotonů v přiměřených izotropních statických prostředích při slabých signálech a interakčních jevech.
The work deals with design and implementation of an analogue electro-optic transmission system convenient to solve some specialized tasks in the field of diagnostics and measurements. The proposed system is designed to solve several requirements that are beyond the limits of conventional metallic transmission medium. Especially it is the case of broadband transmission, extreme length of the transmission medium and high galvanic barrier between input and output that have to be achieved simultaneously. and Práce popisuje vývoj a realizaci analogového elektro-optického přenosového systému použitelného v některých speciálních případech diagnostiky a měření. Navržený přenosový systém řeší několik vzájemně se limitujících požadavků, které není možné standardním metalickým propojením splnit. Jde zejména o současné dosažení velké šířky frekvenčního pásma, délky přenosového média a dostatečné galvanické bariéry mezi vstupem a výstupem.
This paper describes experimental work done on a prototype of in-pipe bristled minimachine, which locomotes in pipe with inner diameter under 40 mm. The principle of minimachine locomotion is based on difference friction force between bristle tip and pipe wall. The new bristle design and its mathematical and graphical model will be presented. and Obsahuje seznam literatury