M2 macrophages expressing CD163 are known to suppress immune responses but have been also found in biopsies of patients with chronic kidney allograft injury associated with interstitial fibrosis. The aim of our study was to evaluate the expression of CD163 in blood monocytes, precursors of tissue macrophages, in kidney allograft recipients with uncomplicated outcome (n=94) compared with those developing acute rejection (n=44). Blood samples were collected before the transplantation and at 1 week, 1 month and 1 year. The expression of CD163 increased during the first week after the transplantation not only in classical (CD14+CD16- ) but also in intermediate (CD14+CD16+) and nonclassical (CD14lowCD16+) monocytes in all patients regardless of their rejection status. In patients developing acute rejection, higher pre-transplant expression of CD163 on blood monocytes was found. In vitro experiments confirmed strong induction of membrane CD163 on monocytes together with CD206 (an alternative marker of M2 macrophages) in response to IL-10. We assume from our data that dramatic upregulation of CD163 by peripheral blood monocytes may have a pathophysiological role in early phases after kidney allograft transplantation and high pre-transplant expression of CD163 on blood monocytes might be involved in events leading to acute rejection., Lenka Čurnová, Kristýna Mezerová, Veronika Švachová, Martina Fialová, Marek Novotný, Eva Čečrdlová, Ondřej Viklický, Ilja Stříž., and Obsahuje bibliografii
Physical exercise instruction sheets are difficult to understand. In general, considerable information is hidden in these types of instruction sheets, which also makes them difficult for machines to understand. Major missing information types include the source and destination location of a human movement. Here we present a Bayesian network to extract the implicit or missing information from typical exercise instruction sheets. We proposed two different kind of Bayesian networks which consists of three and four variables respectively. The network with three variable are designed to for single exercise instruction with single action or pose and the other one designed for single or multiple sentence with two actions or poses. The conditional probability table (CPT) is the backbone of the Bayesian network. At the start, the CPT is updated from our physical exercise instruction sheet corpus (PEISC). Keeping the Action and Bodypart fixed, we have developed our CPT using a unique approach, i.e., crowdsourcing, where we have developed a CPT update system using 13 different exercises consisting of 44 different exercise videos. Using this system based on the rating of a participant of the video the specific variable of that CPT is updated automatically in the Bayesian network. We also updated the Action variable, which consists of 14 different values (action verbs) using crowdsourcing with a human computation approach.
To fulfill the requirements on the laser ranging systems, the soft/hardware package of the Helwan station was significantly modified in period 1982-84. The max. ranging reprate was increased up to 5 pps. The mount pointing accuracy was increased implementing the mechanical inaccuracies software model. The automatical comparison of the time base to the Loran C signal was put into operation.
The main aim of the present investigation was to verify the effects of three overtraining (OT) protocols performed in downhill (OTR/down), uphill (OTR/up) and without inclination (OTR) on the protein levels of Akt (Ser473), AMPKα (Thr172), PGC-1α, plasma membrane GLUT-1 and GLUT-4 as well as on the glycogen contents in mice gastrocnemius. A trained (TR) protocol was used as positive control. Rodents were divided into naïve (N, sedentary mice), control (CT, sedentary mice submitted to the performance evaluations), TR, OTR/down, OTR/up and OTR groups. At the end of the experimental protocols, gastrocnemius samples were removed and used for immunoblotting analysis as well as for glycogen measurements. There was no significant difference between the experimental groups for the protein levels of pAkt (Ser473), pAMPKα (Thr172), PGC-1α, plasma membrane GLUT-1 and GLUT-4. However, the OTR/up protocol exhibited higher contents of glycogen compared to the CT and TR groups. In summary, the OTR/up group increased the gastrocnemius glycogen content without significant changes of pAkt (Ser473), pAMPKα (Thr172), PGC-1α, plasma membrane GLUT-1 and GLUT-4., G. P. Morais, A. Da Rocha, A. P. Pinto, L. Da C. Oliveira, L. G. De Vicente, G. N. Ferreira, E. C. De Freitas, A. S. R. Da Silva., and Seznam literatury
Particles as an inflexible and synsemantic word class have already become a theme of many individual studies. According to the general definition particle indicates a relation of the speaker to the statement, he/she expresses modality of the sentence or emphasizes constituent of a/the sentence. Some of the particles have the validity of sentence, e. g. Ano. Nikoli. Zajisté. So called proper particles cannot stand in the function of another word class: ať, kéž. On the contrary, improper particles can be (according with coocurence) another word class, too: jistě, asi, prý. Goal of this paper is to point in which contexts the particle bohužel, although it is a non-sentence expression, can gain the character of sentence equivalent and which functions its synonyms and antonyms have.
Cough is one of the most important defensive reflexes. However,
extensive non- productive cough is a harmful mechanism leading
to the damage of human airways. Cough is initiated by activation
of vagal afferents in the airways. The site of their convergence is
particularly the nucleus of the solitary tract (nTS). The secondorder neurons terminate in the pons, medulla and spinal cord and
there is also the cortical and subcortical control of coughing. Upper
airway cough syndrome (UACS) – previously postnasal drip
syndrome - is one of the most common causes of chronic cough
together with asthma and gastroesophageal reflux. The main
mechanisms leading to cough in patients with nasal and sinus
diseases are postnasal drip, direct irritation of nasal mucosa,
inflammation in the lower airways, upper airway inflammation and
the cough reflex sensitization. The cough demonstrated by UACS
patients is probably due to hypersensitivity of the upper airways
sensory nerve or lower airways sensory nerve, or a combination of
both. Further studies are needed to clarify this mechanism.