A survey of proteocephalidean cestodes found in the firewood catfish Sorubimichthys planiceps (Spix et Agassiz) from the Amazon River is provided. The following taxa parasitic in S. planiceps are redescribed on the basis of their type specimens and material collected recently in the Amazon River, near the type localities in Brazil, and in Iquitos, Peru: Monticellia lenha Woodland, 1933; Nomimoscolex lenha (Woodland, 1933) (syn. Proteocephalus lenha Woodland, 1933); and Monticellia megacephala Woodland, 1934, for which a new genus, Lenhataenia, is proposed, with L. megacephala (Woodland, 1934) comb. n. as its type and only species. The new genus is a member of the Monticelliinae, i.e. has all genital organs in the cortex, and is most similar to Chambriella in possessing biloculate suckers and lacking a metascolex. It differs in the morphology of the cirrus-sac that contains a strongly coiled, thick-walled internal sperm duct (vas deferens) and a muscular cirrus of the appearance typical of most proteocephalideans, whereas that of Chambriella is sigmoid, with voluminous, tightly sinuous thick-walled internal sperm duct. In addition, Lenhataenia possesses a well developed internal musculature, whereas the internal musculature of Chambriella is weakly developed, formed by a low number of muscle fibres. The scolex morphology and distribution of microtriches of Peltidocotyle lenha (Woodland, 1933) (syn. Othinoscolex lenha Woodland, 1933 and Othinoscolex myzofer Woodland, 1933), Chambriella sp. and Choanoscolex sp. are described using scanning electron microscopy. The two latter taxa may be new for science and are reported from S. planiceps for the first time .
Chronic volume overload (VO) on the left ventricle (LV) augments redox stress and activates matrix metalloproteinase (MMP) which causes the endocardial endothelial-myocyte (EM) disconnection leading to myocardial contractile dysfunction. VO-induced MMP-9 activation impairs cardiac functions, in part by endothelial endocardial apoptosis, but the role of MMP-9 on EM functions remains obscure. We conjecture that chronic VO activates MMP-9 and causes EM uncoupling. Arteriovenous fistula (AVF) was created in genetically identical wild type (WT) mice (FVB/NJ) and MMP-9 knockout mice (MMP-9KO, FVB.Cg-MMP9tm1Tvu/J). Sham-operated mice were used as controls. Before experimentation the phenotype analysis of MMP-9KO mice was carried out. In-gel-gelatin zymography for MMP-9 activation was performed on LV homogenates. The EM functions were determined on LV rings using tissue myobath. We report a decrease in MMP-9 activity in left ventricular myocardial extracts in MMP-9 deficient mice after AVF. The responses to drugs affecting cardiac functions (acetylcholine (Ach), nitroprusside and bradykinin) were attenuated in AVF mice suggesting the impairment of EM coupling. Interestingly, the EM functions were restored in the MMP-9 deficient mice after AVF. We suggest a direct cause-and-effect relationship between MMP-9 activation and EM uncoupling in LV myocardium after chronic VO and the possible involvement of MMP-9 in myocardial contractile performance., K. S. Moshal, W. E. Rodriguez, U. Sen, S. C. Tyagi., and Obsahuje bibliografii a bibliografické odkazy
The relationship between hippocampal function and aging was explored in Wistar rats using taste aversion learning by comparing the performance of adult dorsal hippocampal lesioned and fifteen-month-old intact rats with that of adult intact rats. In experiment 1 the conditioned blocking phenomenon was absent in the hippocampal and the aging rats. Unlike the adult intact rats, the hippocampal and aging rats were not impaired in acquiring a learned aversion to a cider vinegar solution (3 %) presented as a serial compound with a previously conditioned saccharin solution (0.1 %). In experiment 2 both the hippocampal and the aging rats developed reduced aversions to a saline solution (0.5 %) followed by an i.p. injection of lithium chloride (0.15 M; 2 % b.w.) if the taste solution was previously preexposed without consequences. This latent inhibition effect was similar to that seen in intact adult rats. In both experiments, the aging rats exhibited enhanced conventional learned taste aversions. It is concluded that aging is not a unitary process but induces both hippocampal dependent and hippocampal independent complex changes in the functioning of the neural circuits, implementing taste aversion learning., I. Moron, M.A. Ballesteros, A. Candido, M. Gallo., and Obsahuje bibliografii
Spatial variations in regional forest composition are analyzed for the period around 2 000 years before present in the territory of the Czech Republic. The results of pollen analyses at 16 different sites (original data and those published by other authors) form the basis of this study. The results are preliminary because of the small number of sites sampled. This article demonstrates the possibilities of the approach and is the first step to a wider application in the future. The conclusions indicate that pollen analysis is accurate enough in most cases for the reconstruction of past forest composition on a regional scale, and different deposits reflect spatial heterogeneity. Altitude, intensity of human impact, and soil type were the major factors affecting past distribution of forest trees. Oak and hornbeam woodlands, although widely affected by human activity, dominated the lowlands. Beech and silver fir were an important admixture in these communities. Although oak was present at higher altitudes, the occurrence of upland oak woodlands was limited more than indicated by recent geobotanical reconstructions. Instead, mixed forests existed at middle altitudes, often dominated by silver fir and beech. In less favourable habitats, spruce was common. Such upland forests extended high into the mountains, where because of the more severe climatic conditions beech and spruce started to dominate over silver fir.
Holotype and paratype of Ascocotyle (Phagicola) rara Arruda, Muniz-Pereira et Pinto, 2002, a heterophyid trematode recently described on the basis of two worms collected by Lauro Travassos in 1921 in the intestine of Ixobrychus exilis (Gmelin) from Brazil, were studied. The morphology of the worms revealed their conspecificity with Ascocotyle (Phagicola) angeloi Travassos, 1928 found in the same host. Both the taxa have a similar length (between 600 and 900 µm) and shape of the body (long pyriform), the long intestinal caeca reaching to the ovarian level, a long posterior muscular prolongation of the oral sucker and the prepharynx, transverse uterine loops situated between the ventral sucker and testes, and the gonotyl with more than 20 digitiform pockets. Consequently, A. (P.) rara is proposed as a junior synonym of Ascocotyle (Phagicola) angeloi.
Males of the nematode Philometra lateolabracis (Yamaguti, 1935), the type species of the genus Philometra Costa, 1845, were discovered for the first time in gonads of its type host, the Japanese seaperch, Lateolabrax japonicus (Cuvier). Morphological comparisons carried out between the collected male and female P. lateolabracis with the male and female philometrid nematodes previously reported as P. lateolabracis infecting chicken grunt, Parapristipoma trilineatum (Thunberg), and red sea bream, Pagrus major (Temminck et Schlegel), revealed that the latter represent two new species, Philometra isaki sp. n. and Philometra madai sp. n., respectively. Molecular comparison of ITS2 rDNA between P. lateolabracis and P. madai supported the morphological conclusion that the two nematodes obtained from different fish species should be assigned to different species.
Traditionally, all freshwater representatives of red algae with uniaxial cartilagineous and pseudoparenchymatous thalli were placed in the genus Lemanea. Two subgenera of this genus were distinguished, Lemanea and Paralemanea. The recently proposed elevation of these subgenera to genera is fully justified and generally accepted. However, the increasing data from natural populations of Lemanea shows that not all the traditional diacritical features are reliable for distinguishing species. This paper presents the results of a research project on the morphological variability of Lemanea in the Czech Republic. Of the four species Lemanea fluviatilis and L. torulosa appear to be well-defined but there are no clear differences between Paralemanea annulata and P. catenata. A survey of taxa and key to species are presented.
The broader Corinth Gulf region is characterized by a notable active tectonic regime, associated with multiple active fault zones. The continuous N–S extensional tectonics of the area is responsible for the roughly E–W trending active normal fault zones, while individual fault segments are associated with seismic events. Satellite geodesy is a qualitative and quantitative means of estimating the tectonically active setting, based on the recorded motions. The study area is monitored by 14 permanent GPS/GNSS stations, collecting primary geodetic data for a 7-year time period (2008-2014). A 30-sec observation rate was performed, resulting in the extraction of the GPS/GNSS velocity values. The primary geodetic data were processed by applying the triangulation methodology, based on the combination of three different GPS/GNSS stations data, which were considered as the triangle vertices. Triangulation methodology led to the construction of 26 different triangles, while for each of them a series of parameters was determined. In particular, the extracted parameters are: a) Maximum Horizontal Extension, b) Total Velocity, c) Maximum Shear Strain and d) Area Strain. The extracted results are expected to approach, qualitatively and quantitatively, the interpretation of the tectonic regime, as well as to determine new, seismic-related, tectonic features.