Aryl hydrocarbon receptor (AhR) and glucocorticoid receptor (GR) play crucial role in the regulation of drug metabolizing enzymes and in many essential physiological processes. Cellular signaling by these receptors shares several functional and regulatory features. Here we investigated regulatory cross-talk between these two receptors. Human hepatoma cells (HepG2) were the model of choice. We analyzed the effects of dexamethasone (DEX) and dioxin (TCDD) on i) expression of AhR and GRα mRNAs; ii) levels of AhR and GR proteins; iii) transcriptional activities of AhR and GR in reporter assays; iv) 7-ethoxyresorufin-O-deethylase activity (EROD). We found that both DEX and TCDD affected AhR and GR mRNAs expression, proteins levels and transcriptional activities in HepG2 cells. These effects on cellular signaling by AhR and GR comprised up-/down-regulation of gene expression and ligand-dependent protein degradation. We conclude that interactive regulatory cross-talk between GR and AhR receptors in HepG2 cells defines possible implications in physiology and drug metabolism. Future research should be focused on the investigation of AhR-GR cross-talk in various normal human cells and tissues both in vitro and in vivo., Z. Dvořák, R. Vrzal, P. Pávek, J. Ulrichová., and Obsahuje bibliografii a bibliigrafické odkazy
In this paper, a mechanism of adaptive width adjustment based on immunological vaccination is proposed for the evolutionary training of RBF neural networks. Inspired by the vaccination process of the natural immune system, the algorithm implements an individual-orientated adaptation of the width in training stages to optimize the potential solutions, therefore reinforces the evolutionary capability and efficiency. A two-layer genotype-coding scheme, which enables a simultaneous evolution of network structure and parameters, is presented to achieve a compact and consistent-in-form solution. The proposed learning strategy is tested on several benchmark problems and results demonstrate promise.
A novel rriethod that allows us to study the emergence of modularity
for genotype-phenotype mapping in the course of Darwinian evolution is described. The evolutionary method used is based on cornposite chromosomes with two parts; One is a binary genotype whereas the other corresponds to the mapping of genes onto phenotype characters. For such generalized chromosomes the modularity is determined by the following intuitive way: The genes are divided into two subgroups; simultaneously with this decomposition also an accompanied decomposition of the set of phenotype characters is defined. We expect that for chromosomes with rnodular structures the genes frorn one group are rnapped onto characters from the respective group, an appearance of “crosslink” mappings is rnaximally suppressed. A fundamental question for the whole evolutionary biology (and also for evolutioriary algorithms and connectionist cognitive science) is the nature of mechanism of evolutionary emergence of modular structures. An idea of effective fitness is used in the presented explanatory simulations. It is based on the rnetaphor of Hinton and Nowlan theory of the Baldwin eífect, and was ušed as an effective idea for generalization of evolutionary algorithms. The effective fitness reflects not only a static concept of the phenotype, but also its ability to be adapted (learned) within a neighborhood of the respective chromosome. The chromosomes determined in the presented paper inay be understood as objects with the type of plasticity. The rnetaphor of the Baldwin effect (or effective fitness) applied to evolutionary algorithms offers an evolutionary tool that is potentially able to produce the emergence of modularity.
We give an example of a space $X$ with the property that every orientable fibration with the fiber $X$ is rationally totally non-cohomologous to zero, while there exists a nontrivial derivation of the rational cohomology of $X$ of negative degree.
The first explicit example of a positive semidefinite double sequence which is not a moment sequence was given by Friedrich. We present an example with a simpler definition and more moderate growth as $(m, n) \rightarrow \infty $.
A periodic boundary value problem for nonlinear differential equation of the second order is studied. Nagumo condition is not assumed on a part of nonlinearity. Existence and multiplicity results are proved using the method of lower and upper solutions. Results are applied to the generalized Liénard oscillator.
This paper investigates the problem of optimal partitioning of a measurable space among a finite number of individuals. We demonstrate the sufficient conditions for the existence of weakly Pareto optimal partitions and for the equivalence between weak Pareto optimality and Pareto optimality. We demonstrate that every weakly Pareto optimal partition is a solution to the problem of maximizing a weighted sum of individual utilities. We also provide sufficient conditions for the existence of core partitions with non-transferable and transferable utility.
Peritoneal dialysis (PD) is a well established method of depuration in uremic patients. Standard dialysis solutions currently in use are not biocompatible with the peritoneal membrane. Studying effects of dialysate on peritoneal membrane in humans is still a challenge. There is no consensus on the ideal experimental model so far. We, therefore, wanted to develop a new experimental non-uremic rabbit model of peritoneal dialysis, which would be practical, easy to conduct, not too costly, and convenient to investigate the long-term effect of dialysis fluids. The study was done on 17 healthy Chinchilla male and female rabbits, anesthetized with Thiopental in a dose of 0.5 mg/kg body mass. A catheter, specially made from Tro-soluset (Troge Medical GMBH, Hamburg, Germany) infusion system, was then surgically inserted and tunneled from animals' abdomen to their neck. The planned experimental procedure was 4 weeks of peritoneal dialysate instillation. The presented non-uremic rabbit model of peritoneal dialysis is relatively inexpensive, does not require sophisticated technology and was well tolerated by the animals. Complications such as peritonitis, dialysis fluid leakage, constipation and catheter obstruction were negligible. This model is reproducible and can be used to analyze the effects of different dialysis solutions on the rabbit peritoneal membrane., S. Zunic-Bozinovski, Z. Lausevic, S. Krstic, N. Jovanovic, J. Trbojevic-Stankovic, B. Stojimirovic., and Obsahuje bibliografii a bibliografické odkazy
In this paper, a construction method on a bounded lattice obtained from a given t-norm on a subinterval of the bounded lattice is presented. The supremum distributivity of the constructed t-norm by the mentioned method is investigated under some special conditions. It is shown by an example that the extended t-norm on L from the t-norm on a subinterval of L need not be a supremum-distributive t-norm. Moreover, some relationships between the mentioned construction method and the other construction methods in the literature are presented.