Wolves are currently recolonising their historic range in France. The collection of scats is a widely used a non-invasive survey method to monitor wolf population size. However, seasonal changes in wolf faecal deposition patterns might affect the results of surveys. We used a detection dog and camera trapping (CT) to compare wolf scat detectability during winter and the nursing season. We collected 113 scats deposited by adult wolves at 29 marking sites on forest roads in the Sainte-Baume Regional Park, Provence, France. After parturition, the mean number of adult wolf scats increased by 160% inside the nursing territory and decreased by 80% outside of it. Around the time the pups are born, changes in faecal deposition patterns of adults make it easier to find scats around the wolf den (87% probability per wolf marking site) and harder to find scats outside the nursing territory (11% probability). During winter, the chance to find scats is equal (38 to 40% probability per wolf marking site) inside vs. outside the nursing territory. The combined use of a detection dog and camera traps allowed us to gather data on wolf defecation patterns non-invasively. Detectability of adult wolf scats during the nursing season is highly variable compared to winter due to seasonal behavioural changes affecting scat location. We conclude that surveys to collect samples and estimate wolf population size should be conducted exclusively during winter to avoid sampling biases.
Copepods of the genus Achtheinus Wilson, 1908 (Pandaridae) are parasites of elasmobranchs that attach to their fins, gill slits and around the nostrils. Specimens of Achtheinus pinguis Wilson, 1912 were collected and examined using histology and scanning electron microscopy to determine their way of attachment to the host and the possible effect on the host. They insert their antennae deep into the dermis of the shark's skin, which causes the most damage due to possible tissue compression and/or fibrosis as well as rupture of the connective tissue. Additionally, the presence of the copepod on the skin causes cell erosion of the epidermal cells and thus reduces the number of epidermal layers. The maxillipeds are used to attach to the placoid scales that cover the shark's skin and probably serve to keep the copepod and inserted antennae in position. This is accomplished by the insertion of the placoid scales into the flaccid corpus of the maxillipeds. Observed damage seems to be negligible to the shark apart from the possibility of secondary infection., Susan M. Dippenaar, Anine Jordaan., and Obsahuje bibliografii
The whipworms, i.e. parasitic nematodes of the genus Trichuris Roederer, 1761, infect a variety of mammals. Apparently low diversity of primate-infecting species of Trichuris strongly contrasts with the high number of species described in other mammalian hosts. The present study addresses the diversity of whipworms in captive and free-ranging primates and humans by analysing nuclear (18S rRNA, ITS2) and mitochondrial (cox1) DNA. Phylogenetic analyses revealed that primate whipworms form two independent lineages: (i) the Trichuris trichiura (Linnaeus, 1771) clade comprised of genetically almost identical whipworms from human and other primates, which suggests the ability of T. trichiura to infect a broader range of primates; (ii) a clade containing primarily Trichuris suis Schrank, 1788, where isolates from human and various primates formed a sister group to isolates from pigs; the former isolates thus may represent of more species of Trichuris in primates including humans. The analysis of cox1 has shown the polyphyly of the genera Trichuris and Capillaria, Zeder, 1800. High sequence similarity of the T. trichiura isolates from humans and other primates suggests their zoonotic potential, although the extent of transmission between human and other non-human primates remains questionable and requires further study., Jana Doležalová, Miroslav Oborník, Eva Hajdušková, Milan Jirků, Klára J. Petrželková, Petra Bolechová, Cristina Cutillas, Rocio Callejón, Jozef Jaroš, Zuzana Beránková, David Modrý., and Obsahuje bibliografii
Tsetse flies are well-known vectors of trypanosomes pathogenic for humans and livestock. For these strictly blood-feeding viviparous flies, the host blood should be the only source of nutrients and liquids, as well as any exogenous microorganisms colonising their intestine. Here we describe the unexpected finding of several monoxenous trypanosomatids in their gut. In a total of 564 individually examined Glossina (Austenia) tabaniformis (Westwood) (436 specimens) and Glossina (Nemorhina) fuscipes fuscipes (Newstead) (128 specimens) captured in the Dzanga-Sangha Protected Areas, Central African Republic, 24 (4.3%) individuals were infected with monoxenous trypanosomatids belonging to the genera Crithidia Léger, 1902; Kentomonas Votýpka, Yurchenko, Kostygov et Lukeš, 2014; Novymonas Kostygov et Yurchenko, 2020; Obscuromonas Votýpka et Lukeš, 2021; and Wallacemonas Kostygov et Yurchenko, 2014. Moreover, additional 20 (3.5%) inspected tsetse flies harboured free-living bodonids affiliated with the genera Dimastigella Sandon, 1928; Neobodo Vickerman, 2004; Parabodo Skuja, 1939; and Rhynchomonas Klebs, 1892. In the context of the recently described feeding behaviour of these dipterans, we propose that they become infected while taking sugar meals and water, providing indirect evidence that blood is not their only source of food and liquids.
This paper describes the post-disaster reconstruction in the Tohoku region after the 2011 earthquake. Nine years have passed since the Great East Japan Earthquake and Tsunami occurred, and many efforts have been made since to rebuild the devastated territories. Some Japanese architects and urban planners have seen the recovery as a window of opportunity to aim for more resilient cities. Nevertheless, building disaster-resilient communities remains a challenging task. This short paper presents the initiatives made to improve refugees’ social conditions in disaster-relief housing, using the case study of Iwanuma’s relocation project. Concluding remarks suggest that many efforts have been made to improve the social aspect of disaster-relief housing in Japan, for example through the development of community spaces or the pursuit of friendlier dwellings.
To investigate the effect of hydrogen sulfide (H2S) on myocardial injury in sepsis-induced myocardial dysfunction (SIMD), male C57BL/6 mice were intraperitoneally injected with lipopolysaccharide (LPS) (10 mg/kg, i.p.) to induce cardiac dysfunction without or with the H2S donor sodium hydrosulfide (NaHS) (50 µmol/kg, i.p.) administration 3 h after LPS injection. Six hours after the LPS injection, echocardiography, cardiac hematoxylin and eosin (HE) staining, myocardial damage and inflammatory biomarkers and Western blot results were analyzed. In mice, the administration of LPS decreased left ventricular ejection fraction (LVEF) by 30 % along with lowered H2S levels (35 % reduction). It was observed that cardiac troponin I (cTnI), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) levels were all increased (by 0.22-fold, 2000-fold and 0.66-fold respectively). HE staining revealed structural damage and inflammatory cell infiltration in the myocardial tissue after LPS administration. Moreover, after 6 h of LPS treatment, toll-like receptor 4 (TLR4) and nod-like receptor protein 3 (NLRP3) expressions were up-regulated 2.7-fold and 1.6-fold respectively. When compared to the septic mice, NaHS enhanced ventricular function (by 0.19-fold), decreased cTnI, TNF-α, and IL-1β levels (by 11 %, 33 %, and 16 % respectively) and downregulated TLR4 and NLRP3 expressions (by 64 % and 31 % respectively). Furthermore, NaHS did not further improve cardiac function and inflammation in TLR4-/- mice or mice in which NLRP3 activation was inhibited by MCC950, after LPS injection. In conclusion, these findings imply that decreased endogenous H2S promotes the progression of SIMD, whereas exogenous H2S alleviates SIMD by inhibiting inflammation via the TLR4-NLRP3 pathway suppression.
The aim of the present study was to explore whether hydrogen sulfide (H2S) protects against ischemic heart failure (HF) by inhibiting the necroptosis pathway. Mice were randomized into Sham, myocardial infarction (MI), MI + propargylglycine (PAG) and MI + sodium hydrosulfide (NaHS) group, respectively. The MI model was induced by ligating the left anterior descending coronary artery. PAG was intraperitoneally administered at a dose of 50 mg/kg/day for 4 weeks, and NaHS at a dose of 4mg/kg/day for the same period. At 4 weeks after MI, the following were observed: A significant decrease in the cardiac function, as evidenced by a decline in ejection fraction (EF) and fractional shortening (FS), an increase in plasma myocardial injury markers, such as creatine kinase-MB (CK-MB) and cardiac troponin I (cTNI), an increase in myocardial collagen content in the heart tissues, and a decrease of H2S level in plasma and heart tissues. Furthermore, the expression levels of necroptosis-related markers such as receptor interacting protein kinase 1 (RIP1), RIP3 and mixed lineage kinase domain-like protein (MLKL) were upregulated after MI. NaHS treatment increased H2S levels in plasma and heart tissues, preserving the cardiac function by increasing EF and FS, decreasing plasma CK-MB and cTNI and reducing collagen content. Additionally, NaHS treatment significantly downregulated the RIP1/RIP3/MLKL pathway. While, PAG treatment aggravated cardiac function by activated the RIP1/RIP3/MLKL pathway. Overall, the present study concluded that H2S protected against ischemic HF by inhibiting RIP1/RIP3/MLKL-mediated necroptosis which could be a potential target treatment for ischemic HF.
Diabetic foot ulcer (DFU) is a serious complication of diabetes and hyperbaric oxygen therapy (HBOT) is also considered in comprehensive treatment. The evidence supporting the use of HBOT in DFU treatment is controversial. The aim of this work was to introduce a DFU model in ZDF rat by creating a wound on the back of an animal and to investigate the effect of HBOT on the defect by macroscopic evaluation, quantitative histological evaluation of collagen (types I and III), evaluation of angiogenesis and determination of interleukin 6 (IL6) levels in the plasma. The study included 10 rats in the control group (CONT) and 10 in the HBOT group, who underwent HBOT in standard clinical regimen. Histological evaluation was performed on the 18th day after induction of defect. The results show that HBOT did not affect the macroscopic size of the defect nor IL6 plasma levels. A volume fraction of type I collagen was slightly increased by HBOT without reaching statistical significance (1.35±0.49 and 1.94±0.67 %, CONT and HBOT, respectively). In contrast, the collagen type III volume fraction was ~120 % higher in HBOT wounds (1.41±0.81 %) than in CONT ones (0.63±0.37 %; p=0.046). In addition, the ratio of the volume fraction of both collagens in the wound ((I+III)w) to the volume fraction of both collagens in the adjacent healthy skin ((I+III)h) was ~65 % higher in rats subjected to HBOT (8.9±3.07 vs. 5.38±1.86 %, HBOT and CONT, respectively; p=0.028). Vessels density (number per 1 mm2 ) was found to be higher in CONT vs. HBOT (206.5±41.8 and 124±28.2, respectively, p<0.001). Our study suggests that HBOT promotes collagen III formation and decreases the number of newly formed vessels at the early phases of healing., Jiří Růžička, Martina Grajciarová, Lucie Vištejnová, Pavel Klein, Filip Tichánek, Zbyněk Tonar, Jiří Dejmek, Jiří Beneš, Lukáš Bolek, Robert Bajgar, Jitka Kuncová., and Obsahuje bibliografii
Chronic wound is a serious medical issue due to its high prevalence and complications; hyperbaric oxygen therapy (HBOT) is also considered in comprehensive treatment. Clinical trials, including large meta-analyses bring inconsistent results about HBOT efficacy. This review is summarizing the possible effect of HBOT on the healing of chronic wound models at the cellular level. HBOT undoubtedly escalates the production of reactive oxygen and nitrogen radicals (ROS and RNS), which underlie both the therapeutic and toxic effects of HBOT on certain tissues. HBOT paradoxically elevates the concentration of Hypoxia inducible factor (HIF) 1 by diverting the HIF-1 degradation to pathways that are independent of the oxygen concentration. Elevated HIF-1 stimulates the production of different growth factors, boosting the healing process. HBOT supports synthesis of Heat shock proteins (HSP), which are serving as chaperones of HIF-1. HBOT has antimicrobial effect, increases the effectiveness of some antibiotics, stimulates fibroblasts growth, collagen synthesis and suppresses the activity of proteolytic enzymes like matrix metalloproteinases. All effects of HBOT were investigated on cell cultures and animal models, the limitation of their translation is discussed at the end of this review