Accumulating evidence indicates that hypertension is associated with "ion channel remodeling" of vascular smooth muscle cells (VSMCs). The objective of this study was to determine the effects of exercise intensity/volume on hypertension-associated changes in large-conductance Ca2+-activated K+ (BKCa) channels in mesenteric arteries (MAs) from spontaneously hypertensive rats (SHR). Male SHRs were randomly assigned to three groups: a low-intensity aerobic exercise group (SHR-L: 14 m/min), a moderate-intensity aerobic exercise group (SHR-M: 20 m/min), and a sedentary group (SHR). Age-matched Wistar-Kyoto rats (WKYs) were used as normotensive controls. Exercise groups completed an 8-week exercise program. Elevation of the α and β1 proteins was unequal in MA myocytes from SHRs, with the β1 subunit increasing more than the α subunit. BKCa contribution to vascular tone regulation was higher in the myocytes and arteries of SHRs compared to WKYs. SHR BKCa channel subunit protein expression, β1/α ratio, whole cell current density and single-channel open probability was also increased compared with WKYs. Aerobic exercise lowered systemic blood pressure and normalized hypertension-associated BKCa alterations to normotensive control levels in the SHRs. These effects were more pronounced in the moderate-intensity group than in the low-intensity group. There is a dose-effect for aerobic exercise training in the range of low to moderate-intensity and accompanying volume for the correction of the pathological adaptation of BKCa channels in myocytes of MAs from SHR., Y. Zhang, Y. Chen, L. Zhang, N. Lu, L. Shi., and Obsahuje bibliografii
Cytochrome P450s (P450s) involved in insecticide resistance reduce the efficacy of insecticide-based vector control by rendering vector control ineffective. They are recorded in many species of vectors and have various constitutive and insecticide induction profiles. In this study, the isolation and prediction of the structure of a P450 from a strain of Aedes aegypti originating from Malaysia is reported. Quantitative mRNA expression of this gene and a previously reported P450, CYP4H28v2, in the developmental stages of the mosquito after exposure to sub-lethal concentrations of insecticides is also reported. The isolated P450, CYP4H31v2, is an allelic variant of CYP4H31 and contains several conserved motifs of P450s. The secondary structure of the protein is mostly made up of alpha helices and random coils. The tertiary structure was generated using homology modeling and was of good quality based on structure validation using protein structure assessment tools. CYP4H28v2 and CYP4H31v2 were differentially expressed in the developmental stages of the vector, with a significantly increased expression in adult males. The genes were significantly over-expressed in larvae exposed to deltamethrin and permethrin for 6 h. In the DDT-treated larvae, only CYP4H31v2 was significantly over-expressed after a 6 h exposure. Under-expression of the genes was predominant in larvae treated with the organophosphates malathion and temephos. Though the functions of these P450s are unknown, their response to induction by exposure to insecticides indicates the likely involvement of these genes in insecticide tolerance. and Fatma M. A. El-Garj, Mustafa F.F. Wajidi, Silas W. Avicor.
CD163 is a marker of macrophages with anti-inflammatory properties and its soluble form (sCD163) is considered a prognostic predictor of several diseases including type 2 diabetes mellitus (T2DM). We explored sCD163 levels at baseline and after very low-calorie diet (VLCD) or bariatric surgery in 32 patients with obesity (20 undergoing VLCD and 12 bariatric surgery), 32 obese patients with T2DM (22 undergoing VLCD and 10 bariatric surgery), and 19 control subjects. We also assessed the changes of CD163 positive cells of monocyte-macrophage lineage in peripheral blood and subcutaneous adipose tissue (SAT) in subset of patients. Plasma sCD163 levels were increased in obese and T2DM subjects relative to control subjects (467.2±40.2 and 513.8±37.0 vs. 334.4±24.8 ng/ml, p=0.001) and decreased after both interventions. Obesity decreased percentage of CD163+CD14+ monocytes in peripheral blood compared to controls (78.9±1.48 vs. 86.2±1.31 %, p=0.003) and bariatric surgery decreased CD163+CD14+HLA-DR+ macrophages in SAT (19.4±2.32 vs. 11.3±0.90 %, p=0.004). Our data suggest that increased basal sCD163 levels are related to obesity and its metabolic complications. On the contrary, sCD163 or CD163 positive cell changes do not precisely reflect metabolic improvements after weight loss., A. Cinkajzlová, Z. Lacinová, J. Kloučková, P. Kaválková, P. Trachta, M. Kosák, J. Krátký, M. Kasalický, K. Doležalová, M. Mráz, M. Haluzík., and Obsahuje bibliografii
Classical biological control is an important means of managing the increasing threat of invasive plants. It constitutes the introduction of natural enemies from the native range of the target plant into the invaded area. This method may be the only cost-effective solution to control the rapidly expanding common ragweed, Ambrosia artemisiifolia, in non-crop habitats in Europe. Therefore, candidate biocontrol agents urgently need to be assessed for their suitability for ragweed control in Europe. A previous literature review prioritized the host-specific leaf beetle Ophraella slobodkini as a candidate agent for ragweed control in Europe, whereas it rejected its oligophagous congener O. communa. Meanwhile, O. communa was accidentally introduced and became established south of the European Alps, and we show here that it is expanding its European range. We then present a short version of the traditional pre-release risk-benefit assessment for these two candidate agents to facilitate fast decision-making about further research efforts. We selected two complementary tests that can be conducted relatively rapidly and inform about essential risks and benefits. We conducted a comparative no-choice juvenile performance assay using leaves of ragweed and sunflower, the most important non-target plant, in Petri dishes in climatic conditions similar to that in the current European range of O. communa. This informs on the fundamental host range and potential for increasing abundance on these host plants. The results confirm that O. slobodkini does not survive on, and is hence unlikely to cause severe damage to sunflower, while O. communa can survive but develops more slowly on sunflower than on ragweed. In parallel, our species distribution models predict no suitable area for the establishment of O. slobodkini in Europe, while O. communa is likely to expand its current range to include a maximum of 18% of the European ragweed distribution. Based on this early assessment, the prioritization and further assessment of O. slobodkini seem unwarranted whereas the results urgently advocate further risk-benefit analysis of O. communa. Having revealed that most of the European area colonized by ragweed is unlikely to be suitable for these species of Ophraella we suggest the use of such relatively short and cheap preliminary assessment to prioritise other candidate agents or strains for these areas., Suzanne T. E. Lommen, Emilien F. Jolidon, Yan Sun, José I. Bustamante Eduardo, Heinz Müller-Schärer., and Obsahuje bibliografii
The velocities of the Global Positioning System (GPS) stations are widely employed for numerous geodynamical studies. The aim of this paper is to investigate the reliability of station velocities and to draw reader’s attention that for proper estimates of velocity, we need to consider the optimal character of noise. We focus on a set of 115 European GPS stations which contributed to the newest release of the International Terrestrial Reference Frame (ITRF), i.e. ITRF2014. Based on stacked Power Spectral Densities (PSDs), we show that amplitudes o f seasonal signals are significant for nine harmonics of tropical year (365.25 days) and two harmonics of draconitic year (351.60 days). The amplitudes of tropical annual signal fall between 0.1-8.4 mm and are much higher for vertical component than for horizontal. Draconitic annual signal reaches the maximum amplitudes of 1.2 and 0.9 mm for North and East, respectively, whereas is slightly higher for the Up component with a maximum of 3.1 mm. We performed a noise analysis with Maximum Like lihood Estimation (MLE) and found that stations in Central and Northern Europe are characterized by spectral index between flicker and random-walk noise, while stations in Southern and Western Europe: between white and flicker noise. Both amplitudes and spectral indices of power-law noise show a spatial correlation for Up component. We compared the uncertainties of velocities derived in this study with a combination of power-law and white noises to the ones offici ally released in the ITRF2014 with a pure white noise. A ratio of the two estimates is larger than 10 for 13 % and 30 % of stations in horizontal and vertical direction, respectively with medians of 6 and 7. The large differences support the fact that at the velocity determination the proper noise characteristic should be taken into account to avoid any mislead interpretation., Anna Klos and Janusz Bogusz., and Obsahuje bibliografické odkazy
Water is one of the most important components of the environment, having a direct effect on the maintenance of life on the Earth. In this paper, analysis of groundwater level variations, water balance and all the parameters included in these quantities, i.e. precipitation, evapotranspiration, surface run-off and subsurface run-off, were performed in the area of the Sudety Mountains for the period of November 2002 - October 2015. The groundwater level variations were computed on the basis of the mean Terrestrial Water Storage (TWS) values determined from Gravity Recovery and Climate Experiment (GRACE) observations and Global Land Data Assimilation System (GLD AS). TWS data have been determined with a spatial resolution of one degree and temporal resolution of one month. According to the results, groundwater level variation can be approximately determined by water balance changes (with reverse sign). Specifically, for the Sudety area a high average stability of total water storage over the period of past 13 years and decline in groundwater level by about 13 cm (approximately 1 cm/year) was detected., Zofia Rzepecka, Monika Birylo, Joanna Kuczynska-Siehien, Jolanta Nastula and Katarzyna Pajak., and Obsahuje bibliografické odkazy
High temperature alters the internal microstructure of rocks and consequently changes the physical and mechanical properties of rocks. Many studies have been carried out to examine the transformations in the microstructures of rocks under high temperature through near infrared spectroscopy (NIR), Raman spectroscopy, or thermo gravimetric analysis (TGA). The current review synthesizes the data from a number of literatures and summarizes the major transformations of silica rocks under high temperature. The analysis shows that silica rocks starts to lose the water adsorbed in open pores upon heating at about 150 °C. At 200-300 °C, the reaction between SiOH (silanole) in the rocks generates new Si-O-Si bonds as well as H2O, and decreases the open pores. The rocks undergo volume expansion at >550 °C, and the volume contracts and forms new micro pores or cracks which play an important role in the evacuation of the water., Jishi Geng, Qiang Sun, Yuchun Zhang and Yuliang Zhang., and Obsahuje bibliografické odkazy
Hemolytic uremic syndrome (HUS) is a type of thrombotic microangiopathy, in the course of which some patients may develop chronic kidney disease (CKD). It is clinically important to investigate the markers of a poor prognosis. The levels of angiotensinogen (AGT) and interleukin-18 (IL-18) in serum and urine were evaluated. Study was conducted in 29 children with a history of HUS. Serum and urine AGT concentration was significantly higher in children after HUS as compared to the control group. No differences depending on the type of HUS and gender were noted. The serum concentration of IL-18 in children after HUS was significantly lower, whereas in urine did not differ significantly between the sick and healthy children. A negative correlation between the concentration of AGT in serum and albuminuria in patients after HUS was detected. The results indicate that the concentration of AGT in serum and urine in children after HUS increases, which may indicate the activation of the intrarenal renin-angiotensin-aldosterone system. The statement, that AGT may be a good biomarker of CKD after acute kidney injury due to HUS requires prospective studies with follow-up from the acute phase of the disease on a larger group of patients. Reduced IL-18 serum concentration in children after HUS with no difference in its urine concentration may indicate a loss of the protective effects of this cytokine on renal function due to previously occurred HUS., K. Lipiec, P. Adamczyk, E. Świętochowska, K. Ziora, M. Szczepańska., and Obsahuje bibliografii
The use of Continuously Operating Reference Stations, both singly and as part of an active network, is widely employed in surveying, engineering and other geomatics applications, achieving high accuracy positioning even in real time. With an active network the measurements of the reference stations are processed jointly in order to model the errors and compute network RTK corrections. Due to distance dependent errors (ionospheric and tropospheric delays), single base RTK positioning accuracy decreases with increased baseline length. However, the network solution (NRTK) retains the accuracy and the time to fix ambiguities (TTFA) at a constant level. This study aims to contribute to the scientific research on real time positioning based on active networks. In Southern Spain, ERGNSS, a national CORS network that provides GNSS data for post-processing and real time single- b ased reference station corrections, shares territory with the Andalusian Positioning Network (RAP), a local active network. RAP provides network an d single-based RTK corrections. In order to analyze the quality of real time positioning based on both networks, several tests have been performed on a sample of test points. The reference frame, the time to fix ambiguity resolution, precision, accuracy and repeatability of RTK positioning are considered as the evaluation parameters. The results confirm that the RAP network complements the precise positioning services provided by the ERGNSS network, ensuring accurate real time positioning, full cove rage and reliable positioning services in the Andalusian Community., Mª Selmira Garrido, Antonio J. Gil and Rafael Gaitán., and Obsahuje bibliografické odkazy
Flowers of dicotyledonous plants host communities of arthropod species. We studied the community associated with dandelion (Taraxacum section Ruderalia), a complex of apomictic micro-species abundant in central Europe. Identification of microspecies in the field was impracticable. These plants produce an abundance of flowers that host arthropod communities that are not yet fully documented. We investigated species occurrence, its diurnal and seasonal variation and some of the factors that determine the abundance of the dominant species. Insect and spiders were collected from 2010 to 2012 at a locality in Prague. Whole capitula were harvested at weekly intervals and resident arthropods were identified. Diurnal variation in insect presence and the effect of pollen and microclimate on some of the species were also examined. The insect community (> 200 species) consisted mainly of species of Hymenoptera (86 spp.), Coleoptera (56 spp.), Diptera (46 spp.) and Heteroptera (23 spp.). The most abundant were Thysanoptera (2 spp.). Pollen eaters/collectors and nectar feeders dominated over predators and occasional visitors. From April to mid-August, the insect community was dominated by Coleoptera, and later by Diptera and Hymenoptera. Except for Meligethes spp. and species breeding in the capitula, the insects occupied flowers during the daytime when the flowers were open (10-12 h in spring and only 2-4 h in late summer). The presence of Meligethes spp. in particular flowers was associated with the presence of pollen; the occurrence of Byturus ochraceus with pollen and flower temperature. Although pollination is not necessary, dandelion plants produce both nectar and pollen. The community of arthropods that visit dandelion flowers is rich despite their being ephemeral.The composition of local faunas of flower visitors, presence of floral rewards and flower microclimate are important factors determining the composition of the flower community., Alois Honěk, Zdenka Martinková, Jiří Skuhrovec, Miroslav Barták, Jan Bezděk, Petr Bogusch, Jiří Hadrava, Jiří Hájek, Petr Janšta, Josef Jelínek, Jan Kirschner, Vítězslav Kubáň, Stano Pekár, Pavel Průdek, Pavel Štys, Jan Šumpich., and Obsahuje bibliografii