Number of results to display per page
Search Results
212. A new species of Nothobomolochus (Copepoda: Bomolochidae) parasitic on Engraulis anchoita (Pisces: Engraulidae) from Argentina
- Creator:
- Timi, Juan Tomás and Sardella, Norma Haydée
- Format:
- Type:
- model:internalpart and TEXT
- Subject:
- Nothobomolochus cresseyi sp. п., parasitic Copepoda, and Engraulis anchoita
- Language:
- English
- Description:
- Nothobomolochus cresseyi sp. n. (Copepoda: Bomolochidae), a parasite from gills of the engraulid fish Engraulis anchoita Hubbs et Marini (Pisces: Engraulidae) from the coast of Argentina, is described and illustrated. The new species can be distinguished from other species of Nothobomolochus Vervoort, 1962 by the following combination of characters: three modified setae on the base of the antennule similar in length to the plumose setae, the nature of the armature of the third segment of the fourth endopod and the length and width of the thoracic somites 2 to 4 decreasing gradually. The new species represents the first record of this genus for Argentina.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
213. A new species, Gnathia nkulu sp. n. (Crustacea: Isopoda: Gnathiidae) from southern Africa
- Creator:
- Smit, Nico J. and Van As, Jo G.
- Format:
- Type:
- model:internalpart and TEXT
- Subject:
- Gnathia nkulu, description, taxonomy, and morphology
- Language:
- English
- Description:
- A new species, Gnathia nkulu sp. n. is described from material collected off the South African coast at 80-200m depth. It differs from the intertidal species Gnathia africana Barnard, 1914 in that the mediofrontal process is not deeply divided into two lobes, article 2 of the pylopod is rounded and small wart-like tubercles and long simple setae are present on both the cephalosome and pereon.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
214. A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species
- Creator:
- Gerba, Madougou, Loiseau, Anne, Benoit, Laure, and Gauthier, Nathalie
- Format:
- print, počítač, and online zdroj
- Type:
- article, články, journal articles, model:article, and TEXT
- Subject:
- Zoologie, blanokřídlí, lumčíkovití, populační genetika, Hymenoptera, Braconidae, population genetics, Habrobracon hebetor, Bracon spp., biological control, parasitic wasp, microsatellite, gene flow, 2, and 59
- Language:
- English
- Description:
- Combining a biotin-enrichment protocol and 454GS-FLX titanium pyrosequencing technology, we characterised 22 polymorphic microsatellite loci from the parasitic wasp, Habrobracon hebetor (Say) (Hymenoptera: Braconidae), a cosmopolitan species commonly used in biological control against a wide range of both major lepidopterous pests of stored products and field crops in different parts of the world. Three multiplex PCR sets were optimised and characterised across 46 H. hebetor specimens from two samples collected from millet fields in Niger. Two to 11 alleles were found per locus and observed heterozygosity ranged from 0.289 to 0.826. Polymorphism was detected in both samples with a similar level of observed heterozygosity (0.482 vs. 0.502) and number of alleles (4.1 vs. 3.6). Deviation from Hardy-Weinberg equilibrium was detected at the same five loci in both samples and five or seven more loci in each sample but was not associated with heterozygote deficiencies. Even though evidence for linkage disequilibrium was found between a few alleles, these new loci segregated independently. The variability of the 22 loci will enable estimates of genetic diversity and structure patterns, as well as gene flow between H. hebetor populations at different spatial scales. Cross-species amplifications were successful among the six Bracon spp. tested and nine loci will be particularly appropriate for population genetic studies in B. brevicornis., Madougou Garba, Anne Loiseau, Laure Benoit, Nathalie Gauthier., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
215. A non-invasive method for detecting the metabolic stress response in rodents: characterization and disruption of the circadian corticosterone rhythm
- Creator:
- Thanos, P. K., Cavigelli, S. A., Michaelides, M., Olvet, D. M., Patel, U., Diep, M. N., and Volkow, N. D.
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Biochemie. Molekulární biologie. Biofyzika, fyziologie, stres (fyziologie), metabolity, cirkadiánní rytmy, physiology, stress (physiology), metabolites, circadian rhythms, corticosterone, fecal steroid metabolites, 2, and 577
- Language:
- English
- Description:
- Plasma corticosterone (CORT) measures are a common procedure to detect stress responses in rodents. However, the procedure is invasive and can influence CORT levels, making it less than ideal for monitoring CORT circadian rhythms. In the current paper, we examined the applicability of a non-invasive fecal CORT metabolite measure to assess the circadian rhythm. We compared fecal CORT metabolite levels to circulating CORT levels, and analyzed change in the fecal circadian rhythm following an acute stressor (i.e. blood sampling by tail veil catheter). Fecal and blood samples were collected from male adolescent rats and analyzed for CORT metabolites and circulating CORT respectively. Fecal samples were collected hourly for 24 h before and after blood draw. On average, peak fecal CORT metabolite values occurred 7-9 h after the plasma CORT peak and time-matched fecal CORT values were well correlated with plasma CORT. As a result of the rapid blood draw, fecal production and CORT levels were altered the next day. These results indicate fecal CORT metabolite measures can be used to assess conditions that disrupt the circadian CORT rhythm, and provide a method to measure long-term changes in CORT production. This can benefit research that requires long-term glucocorticoid assessment (e.g. stress mechanisms underlying health)., P. K. Thanos ... [et al.]., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
216. A novel carboxymethylated mercaptotriazinoindole inhibitor of aldose reductase interferes with the polyol pathway in streptozotocin-induced diabetic rats
- Creator:
- Soltesova Prnova, M., Ballekova, J., Alena Gajdošíková, Gajodsik, A., and Stefek, M.
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, diabetici, diabetics, carboxymethylated mercaptotriazinoindole, aldose reductase inhibitor, polyol pathway, diabetic complications, 14, and 612
- Language:
- English
- Description:
- The aim of the present work was to study the effect of 3- mercapto-5H-1,2,4-triazino[5,6-b]indole-5-acetic acid (CMTI), an efficient aldose reductase inhibitor, on sorbitol accumulation in selected organs of streptozotocin-induced diabetic rats in vivo . In addition, the effect of CMTI on aldose reductase back reaction and on sorbitol dehydrogenase was determined. The model of experimental diabetes in male Wistar rats induced by streptozotocin was used. Experimental diabetes was induced by triple intraperitoneal doses of streptozotocin on three consecutive days. In diabetic rats, significant elevation of sorbitol concentration in the sciatic nerve and eye lenses was recorded. CMTI administered intragastrically (50 mg/kg/day) for five consecutive days significantly inhibited sorbitol accumulation in the sciatic nerve, yet it was without effect in eye lenses of diabetic animals. For aldose reductase back reaction, the substrate affinity of glycerol to aldose reductase was one order lower than that of glyceraldehyde in forward reaction. In addition, the back reaction was much slower, characterized by Vmax value of about 30 times lower than that of the forward reaction. Inhibition of aldose reductase by CMTI was characterized by closely related IC50 values in submicromolar range for both forward and back reactions. No significant inhibition of the second enzyme of the polyol pathway, sorbitol dehydrogenase, by 100 μM CMTI was recorded (I=0.9±2.7 %, n=3). To conclude, the presented results showed the ability of CMTI to affect the polyol pathway in diabetic rats in vivo and represent thus a further step in a complex preclinical evaluation of CMTI as a potential agent for treatment of chronic diabetic complications., M. Soltesova Prnova, J. Ballekova, A. Gajdosikova, A. Gajdosik, M. Stefek., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
217. A novel method for the estimation of soybean chlorophyll content using a smartphone and image analysis
- Creator:
- Rigon, J. P. G., Capuani, S., Fernandes, D. M., and Guimarães, T. M.
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- chlorofyl, karotenoidy, chlorophyll, carotenoids, mathematical models, fotosyntetické pigmenty, přenosná zařízení, quinoa, sezam, sójové boby, ricinový olej, tmavě zelený barevný index, vegetační index, smart phone camera, color model, photosynthetic pigments, portable equipment, sesame, soybean, castor oil, pod formation stage, Surinam cherry, dark green color index, vegetation index, 2, and 581
- Language:
- Multiple languages
- Description:
- The development of smartphones, specifically their cameras, and imaging technologies has enabled their use as sensors/measurement tools. Here we aimed to evaluate the applicability of a fast and noninvasive method for the estimation of total chlorophyll (Chl), Chl a, Chl b, and carotenoids (Car) content of soybean plants using a smartphone camera. Single leaf disc images were obtained using a smartphone camera. Subsequently, for the same leaf discs, a Chl meter was used to obtain the relative index of Chl and the photosynthetic pigments were then determined using a classic method. The RGB, HSB and CIELab color models were extracted from the smartphone images and correlated to Chl values obtained using a Chl meter and by a standard laboratory protocol. The smartphone camera was sensitive enough to capture successfully a broad range of Chl and Car contents seen in soybean leaves. Although there was a variation between color models, some of the proposed regressions (e.g., the S and b index from HSB and Lab color models and NRI [RGB model]) were very close to the Chl meter values. Based on our findings, smartphones can be used for rapid and accurate estimation of soybean and Car contents in soybean leaves., J. P. G. Rigon, S. Capuani, D. M. Fernandes, T. M. Guimarães., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
218. A periadventitial sirolimus-releasing mesh decreased intimal hyperplasia in a rabbit model
- Creator:
- Ivo Skalský, Elena Filová, Ondrej Szárszoi, Martin Pařízek, Lytvynets, A., Jana Malušková, Alena Lodererová, Eduard Brynda, Věra Lisá, Burdíková, Z., Martin Čapek, Jan Pirk, and Lucie Bačáková
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, stenózy, hyperplazie, stenoses, hyperplasia, intimal hyperplasia, controlled drug release, perivascular mesh, rapamycin, 14, and 612
- Language:
- English
- Description:
- Autologous vein grafts used as aortocoronary bypasses are often prone to intimal hyperplasia, which results in stenosis and occlusion of the vein. The aim of this study was to prevent intimal hyperplasia using a newly developed perivascular system with sustained release of sirolimus. This system of controlled drug release consists of a polyester mesh coated with a copolymer of L-lactic acid and ε -caprolactone that releases sirolimus. The mesh is intended for wrapping around the vein graft during surgery. The mesh releasing sirolimus was implanted in periadventitial position onto arteria carotis communis of rabbits, and neointimal hyperplasia was then assessed. We found that implanted sirolimus-releasing meshes reduced intima thickness by 47±10 % compared to a vein graft after 3 weeks. The pure polyester mesh decreased vein intima thickness by 35±9 %. Thus, our periadventitial system for controlled release of sirolimus prevented the develo pment of intimal hyperplasia in autologous vein grafts in vivo in rabbits. A perivascularly applied mesh releasing sirolimus is a promising device for preventing stenosis of autologous vein grafts., I. Skalský ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
219. A phylogenetic analysis by multilocus enzyme electrophoresis and multiprimer random amplified polymorphic DNA fingerprinting of the Leishmania genome project Friedlin reference strain
- Creator:
- Baňuls, Andre-Laure, Brisse, Sylvain, Sidibé, Issa, Noel, Sébastien, and Tibayrene, Michal
- Format:
- Type:
- model:internalpart and TEXT
- Subject:
- Leishmania major, MLEE, RAPD, genetic characterization, and genetic diversity
- Language:
- English
- Description:
- We have assessed the phylogenetic status of the Leishmania genome project Friedlin reference strain by MLEE and multiprimer RAPD including a set of 9 stocks representative of the main Leishmania species and of the whole genetic diversity of the Leishmania genus. To our knowledge, the detailed genetic characterization of the Friedlin strain has never been published before. As previously recorded (Tibayrenc et al. 1993), MLEE and RAPD data gave congruent phylogenetic results. The Friedlin reference strain was definitely attributed to Leishmania (Leishmania) major Yakimoff et Schokhor, 1914. Five specific RAPD patterns made it possible to distinguish between the Friedlin strain and the 2 other L. (L.) major stocks included in the study. Various specific MLEE and RAPD characters permitted to distinguish between the Leishmania species included in the study. All these characters are usable to detect accidental laboratory mix-ups involving the Friedlin reference strain. In confirmation with previous studies involving a more limited set of genetic markers, the general genetic diversity of the Leishmania genus proved to be considerable. It must be made clear that only one strain cannot be considered as representative of the whole genetic variability of the genus Leishmania. In the future, it is therefore advisable to complement the results obtained in the framework of the Leishmania genome project with data from other strains that should be selected on a criterion of important genetic differences with the Friedlin strain.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
220. A pioneer of the renewal of Czechoslovak sociology in the 1960s - remembering Josef L. Porket
- Creator:
- Večerník, Jiří
- Format:
- Type:
- model:internalpart and TEXT
- Language:
- Czech
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public