Number of results to display per page
Search Results
23332. Transgenic expression of CD36 in the spontaneously hypertensive rat is associated with amelioration of metabolic disturbances but has no effect on hypertension
- Creator:
- Michal Pravenec, Vladimír Landa, Václav Zídek, Musilová, M., Ludmila Kazdová, Qi, N., Wang, J., Lezin, E. St., and Kurzt, T. W.
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, mastné kyseliny, krevní tlak, inzulinová rezistence, fatty acids, blood pressure, insulin resistance, SHR, transgenic animal, Cd36, 14, and 612
- Language:
- English
- Description:
- Spontaneously hypertensive rats (SHR/NIH strain) harbor a deletion variant in the Cd36 fatty acid transporter and display defective fatty acid metabolism, insulin resistance and hypertension. Transgenic rescue of Cd36 in SHR ameliorates insulin resistance and improves dyslipidemia. However, the role of Cd36 in blood pressure regulation remains controversial due to inconsistent blood pressure effects that were observed with transgenic expression of Cd36 on the SHR background. In the current studies, we developed two new SHR transgenic lines, which express wild type Cd36 under the control of the universal Ef-1 promoter, and examined the effects of transgenic expression of wild type Cd36 on selected metabolic and cardiovascular phenotypes. Transgenic expression of Cd36 in the new lines was associated with significantly decreased serum fatty acids, amelioration of insulin resistance and glucose intolerance but failed to induce any consistent changes in blood pressure as measured by radiotelemetry. The current findings confirm the genetic association of defective Cd36 with disordered insulin action and fatty acid metabolism in the SHR/NIH strain and suggest that Cd36 is linked to other gene(s) on rat chromosome 4 that regulate blood pressure., M. Pravenec, V. Landa, V. Zídek, A. Musilová, L. Kazdová, N. Qi, J. Wang, E. St.Lezin, T. W. Kurtz., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
23333. Transgenic reporter mice with promoter region of murine LRAT specifically marks lens and meiosis spermatocytes
- Creator:
- Dana Průková, Ileninova, Z., Antosova, B., Petr Kašpárek, Gregor, M., and Radislav Sedláček
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie, oči, physiology, eyes, LRAT, transgenic mouse, retinol, testis, spermatocyte, sertoli cell, lens, 14, and 612
- Language:
- English
- Description:
- Lecithin:retinol acyltransferase (LRAT) is the major enzyme responsible for retinol esterification in the mammalian body. LRAT exhibits specific activity in the cells with active retinol metabolism where it converts retinols into retinyl esters, which represents the major storage form of retinol. Besides hepatic stellate cells in the liver, LRAT appears to have a key physiologic role in several other tissues. In this study, we generated a transgenic reporter mouse expressing green fluorescence protein (EGFP) under the control of region containing -1166 bps from promoter upstream from the putative transcriptional start site and 262 bps downstream of this start. Transgenic reporter mice exhibited specific expression in eyes and testes. In eyes, expression of EGFP-reporter is found in lens and lens epithelium and fibers from embryo to adulthood. In testes, LRAT-EGFP reporter is expressed both in Sertoli and in spermatocytes marking initiation of spermatogenesis in prepubertal mice. Our data show that the examined LRAT regulatory region is sufficient to achieve strong and selective expression in the eye and testes but not in liver and other organs., D. Prukova, Z. Ileninova, B. Antosova, P. Kasparek, M. Gregor, R. Sedlacek., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
23334. Transient and non-transient intussusceptions of the large bowel in adults: two case reports
- Creator:
- Vobořil, René, Fanta, Jan, Bačkovský, Petr, Ehrenberger, Daniel, and Weberová, Jana
- Format:
- print, text, and regular print
- Type:
- model:article, article, Text, kazuistiky, časopisecké články, and TEXT
- Subject:
- staří, kolektomie--metody, kolon, nádory tračníku--komplikace--patologie--chirurgie, kolorektální nádory--komplikace--patologie--chirurgie, chybná diagnóza--prevence a kontrola, ženské pohlaví, lidé, nemoci ilea--diagnóza--etiologie--patofyziologie--chirurgie, intususcepce--diagnóza--etiologie--patofyziologie--chirurgie, mužské pohlaví, lidé středního věku, posouzení stavu pacienta, nemoci rekta--diagnóza--etiologie--patofyziologie--chirurgie, spontánní remise, počítačová rentgenová tomografie, and výsledek terapie
- Language:
- English
- Description:
- BACKGROUND: Intussusception of the large bowel in adults is a very rare pathological condition. However, it has its clinical importance because intussusception is very often associated with an intraluminal lesion. CASE REPORT: We report two cases of the large bowel intussusception, ileocolic and colorectal. Both intussusceptions were associated with a malignant tumor. However, the clinical presentation was different. One of the intussusceptions was of non-transient character, while the second one resolved spontaneously before operation. Both patients underwent surgery and malignant tumors were found and removed. RESULTS: The purpose of the article is to draw attention to intussusception and emphasize that intussusception, either transient or non-transient, should be further examined. CONCLUSIONS: The intussusception may be the first and the only signal of the existence of a malignant tumor, very often colorectal carcinoma. and R. Vobořil, J. Fanta, P. Bačkovský, D. Ehrenberger, J. Vobořilová
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
23335. Transient changes of cortical interhemispheric responses after repeated caffeine administration in immature rats
- Creator:
- Tchekalarova, J., Hana Kubová, and Pavel Mareš
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, evokované potenciály, mozková kůra, kofein, evoked potentials, cerebral cortex, caffeine, development, rat, 14, and 612
- Language:
- English
- Description:
- Repeated postnatal caffeine treatment of rat pups led to transient developmental changes in cortical epileptic afterdischarges. To know if physiological cortical functions are also affected transcallosal evoked potentials were studied. Rat pups of the Wistar strain were injected daily with caffeine (10 or 20 mg/kg s.c.) from postnatal day (P) 7 to P11, control siblings received saline. Cortical interhemispheric responses were tested at P12, 18, 25 and in young adult rats. Amplitude of initial monosynaptic components was evaluated in averaged responses. Single pulses as well as paired and frequency (five pulses) stimulations were used. Developmental rules - highest amplitude of responses in 25-day-old rats, potentiation with paired and frequency stimulation present since P18 - were confirmed. Caffeine-treated rats exhibited transient changes: single responses were augmented in P25 if high stimulation intensity was used, paired-pulse and freque ncy responses were higher in experimental than in control anim als at P12, the opposite change was observed in 18- and more ma rkedly in 25-day-old rats. No significant changes were found in adult animals, monosynaptic transcallosal responses represent a simple and robust system. The developmental profile of described changes did not exactly correspond to changes in epileptic afterdischarges supporting the possibility that afterdischarges did not arise from early monosynaptic components of responses. In spite of transient nature of changes they can reflect delayed or more probably modified brain development., J. Tchekalarova, H. Kubová, P. Mareš., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
23336. Transient hypertension and sustained tachycardia in mice housed individually in metabolism cages
- Creator:
- Hoppe, C. C., Moritz, K. M., Fitzgerald, S. M., Bertram, J. F., and Evans, R. G.
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie, myši, stres (fyziologie), hypertenze, sex, physiology, mice, stress (physiology), hypertension, metabolic cage, 14, and 612
- Language:
- English
- Description:
- The novel environment of a metabolic cage can be stressful for rodents, but few studies have attempted to quantify this stress-response. Therefore, we determined the effects on mean arterial pressure (MAP) and heart rate (HR), of placing mice of both sexes in metabolism cages for 2 days. After surgical implantation of a carotid artery catheter mice recovered individually in standard cages for 5 days. Mice then spent 2 days in metabolism cages. MAP and HR were monitored in the standard cage on Day 5 and in metabolism cages on Days 6-7. MAP increased by 18±3 and 22±4 %, while HR increased by 27±4 and 27±6 %, in males and females, respectively, during the first hours after cage switch. MAP decreased to baseline in the fourth and eighth h following metabolism cage switch in males and females, respectively. However, HR remained significantly elevated in both sexes during the entire two-day period in metabolism cages. Females had lower MAP than males both pre- and post- metabolism cage switch, but there were no sex differences in HR. These results demonstrate sustained changes in cardiovascular function when mice are housed in metabolism cages, which could potentially affect renal function., C. C. Hoppe ... [et al.]., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
23337. Transient upregulation of protein kinase C in pressure-overloaded neonatal rat myocardium
- Creator:
- Blanka Hamplová, František Novák, František Kolář, and Olga Nováková
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, proteinové kinázy, protein kinases, protein kinase C, pressure overload, postnatal development, rat myocardium, 14, and 612
- Language:
- English
- Description:
- Protein kinase C (PKC) appears to play a significant role in the signal transduction of cardiac growth and development. The aim of this study was to determine changes in the total PKC activity and the expression of PKC isoforms α, δ and ε in the rat heart that was affected by pressure overload imposed at postnatal day (d) 2. Three groups of Wistar rats were employed for the experiment: rats submitted to the abdominal aortic constriction (AC), sham-operated controls (SO) and intact controls. Animals were sacrificed at d2, d3, d5 and d10. The total PKC activity was measured by the incorporation of 32P into histone IIIS and the expression of PKC was analyzed by immunoblotting in the homogenate of the left ventricular myocardium and in the cytosolic, membrane-enriched (105 × g) and nuclear-cytoskeletalmyofilament- enriched (103 × g) fractions. We observed the significant transient increase in both the total PKC activity and the expression of all isoforms at d5 (the 3rd day after the operation) in the cardiac homogenate of AC rats as compared with SO animals. Aortic constriction did not significantly affect the distribution of activity and isoform abundance among individual cellular fractions except for PKCδ, which increased significantly at d10 in the cytosolic fraction at the expense of the membraneenriched fraction. It is concluded that PKCα, PKCδ and PKCε undergo transient upregulation associated with the accelerated cardiac growth induced by pressure overload imposed in the very early postnatal period., B. Hamplová ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
23338. Transition economies: Dynamic bargaining in new markets
- Creator:
- Derviz, Alexis
- Publisher:
- CERGE-Charles University
- Format:
- print and 42 s.
- Type:
- model:monograph and TEXT
- Subject:
- Národní hospodářství a hospodářská politika, ekonomická transformace, inovace, modelování a simulace, tržní ekonomika, 338, 338.242, 330.341.1, 519.86/.87, (048.8), 4, and 330.5+338
- Language:
- English
- Description:
- Alexis Derviz.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
23339. Transitions in the thylakoid membranes of barley leaves studied by chlorophyll fluorescence temperature curve
- Creator:
- Nauš, J., Dvořák, L., Kuropatwa, R., and Mašláň, M.
- Format:
- Type:
- model:internalpart and TEXT
- Language:
- Multiple languages
- Description:
- Chlorophyll (Chl) fluorescence temperature cinve (FTC) of primary leaves of spring barley (Hordeum vulgare L.), cultivated imder a low irradiance, was studied at low actinic radiation excitation (436 nm, 2 W m'^) under a heating regime consisting of a linear increase of temperature (0.083 °C s’*), followed by a period of constant temperature. After prompt cooling of the sample to 77 K, Chl fluorescence spectra at defined points of the temperature regime were measured. Two distinct temperature and kinetic regions expressed by two discemible temperature maxima were observed for ťhis type of the FTC. Two triggering temperatures of successive irreversible changes are postulated at 45-48 “C and 53-55 °C temperature intervals, respectively; they may correlate with the heat inactivation of photosystem 2 and with changes of the chloroplast and cytoplasmic membrane integrity.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
23340. TRANSITUS EX HOC MUNDO AD PATREM ... DOMINI FERDINANDI, è COMITIBUS DE KHŰNBURG ... FUNEBRI DICTIONE ...
- Creator:
- Platzer, Johann Norbert, Matěj Adam Höger, and Arcibiskupská tiskárna (Praha, Česko)
- Publisher:
- Tiskárna arcibiskupská and Höger, Matěj Adam - factor
- Format:
- print and [8] ff ; 4°
- Type:
- model:monograph and TEXT
- Subject:
- archiepiscopus Pragensis, Khünburg von, Ferdinand -, 1649-1731, století 18., and homiletika
- Language:
- Latin
- Description:
- BCBT00970
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public