a1_The halophytic C4 grass, Aeluropus littoralis, was cultivated under low (50 mM) and high (200 mM) NaCl salinity and inoculated with the arbuscular mycorrhizal fungi (AMF) Claroideoglomus etunicatum in a sand culture medium for 20 weeks. Shoot and root dry mass increased under salinity conditions up to 24 and 86%, respectively. Although the root colonization rate significantly decreased in the presence of salt, AMF-colonized (+AMF) plants had higher biomass compared with plants without AMF colonization (-AMF) only under saline conditions. Net CO2 assimilation rate increased significantly by both salinity levels despite stable stomatal opening. In contrast, AMF-mediated elevation of the net CO2 assimilation rate was associated with a higher stomatal conductance. Unexpectedly, leaf activity of phosphoenolpyruvate carboxylase decreased by salinity and AMF colonization. Transpiration rate was not affected by treatments resulting in higher water-use efficiency under salinity and AMF conditions. Concentrations of soluble sugars and free α-amino acids increased by both salinity and AMF treatments in the shoot but not in the roots. Proline concentration in the leaves was higher in the salt-treated plants, but AMF colonization did not affect it significantly. Leaf activity of nitrate reductase increased by both salinity and AMF treatments. Mycorrhizal plants had significantly higher Na+ and K+ uptake, while Ca2+ uptake was not affected by salt or AMF colonization. The ratio of K+/Na+ increased by AMF in the shoot while it decreased in the roots. Leaf osmotic potential was lowered under salinity in both +AMF and -AMF plants. Our results indicated that higher dry matter production in the presence of salt and AMF could be attributed to higher CO2 and nitrate assimilation rates in the leaves., a2_Higher leaf accumulation of soluble sugars and α-amino acids but not proline and elevated water-use efficiency were associated with the improved growth of A. littoralis inoculated with AMF., R. Hajiboland, F. Dashtebani, N. Aliasgharzad., and Obsahuje seznam literatury
Macroalgae must be able to survive in conditions of different light intensities with no damage to their physiological performance or vital processes. Irradiance can stimulate the biosynthesis of certain photoprotective compounds of biotechnological interest, such as pigments and proteins. Pterocladiella capillacea is a shade-grown alga, which play a role key in the balance of marine ecosystems. In addition, it is considered one of the best sources of bacteriological agar and agarose with a wide pharmacological potential. In order to evaluate the photosensitivity in P. capillacea under 60 (control) and moderate light intensity of 300 μmol(photon) m-2 s-1, photosynthetic performance and chemical composition were assessed. P. capillacea showed photosensitivity without evidence of photodamage. The results indicate the possibility to increase a growth rate and probably infer productivity in long-term cultivation by stimulation at moderate light intensity. Increasing photosynthetic pigment and protein contents were also observed under medium light, an interesting result for functional ingredient approaches., T. B. Harb, A. Nardelli, F. Chow., and Obsahuje bibliografii
To understand the contribution of potassium (K+) channels, particularly α-dendrotoxin (D-type)-sensitive K+ channels (Kv.1, Kv1.2 or Kv1.6 subunits), to the generation of neuronal spike output we must have detailed information of the functional role of these channels in the neuronal membrane. Conventional intracellular recording methods in current clamp mode were used to identify the role of α-dendrotoxin (α-DTX)-sensitive K+ channel currents in shaping the spike output and modulation of neuronal properties of cerebellar Purkinje neurons (PCs) in slices. Addition of α-DTX revealed that D-type K+ channels play an important role in the shaping of Purkinje neuronal firing behavior. Repetitive firing capability of PCs was increased following exposure to artificial cerebrospinal fluid (aCSF) containing α-DTX, so that in response to the injection of 0.6 nA depolarizing current pulse of 600 ms, the number of action potentials insignificantly increased from 15 in the presence of 4-AP to 29 action potentials per second after application of DTX following pretreatment with 4-AP. These results indicate that D-type K+ channels (Kv.1, Kv1.2 or Kv1.6 subunits) may contribute to the spike frequency adaptation in PCs. Our findings suggest that the activation of voltage-dependent K+ channels (D and A types) markedly affect the firing pattern of PCs., H. Haghdoust, M. Janahmadi, G. Behzadi., and Obsahuje bibliografii a bibliografické odkazy
V roce 2010 byl v České republice poprvé zaznamenán výskyt pilatky azalkové (Nematus lipovskyi), jejichž housenice způsobují holožíry na měkkolistých pěnišnících. Druh byl již zaznamenán na více než 60 lokalitách především v Praze a ve středních Čechách, příčemž jde o první zaznamenaný výskyt druhu mimo území USA. V botanické zahradě Přírodovědecké fakulty Univerzity Karlovy v Praze byli poprvé odchyceni samci tohoto druhu., The Azalea sawfly (Nematus lipovskyi), previously known from the eastern states of the USA, was first recorded in the Czech Republic. Since the discovery of the species in 2010 in the botanical garden of Charles University in Prague, more than 60 localities of Nematus lipovskyi have been confirmed, mostly from Prague and Central Bohemia. Males of the species were found for the first time since the description of the species., and Petr Šípek, Ján Macek.
Plachetnatka nákorní (Drapetisca socialis) z čeledi plachetnatkovití (Linyphiidae) je jedním z mála druhů pavouků žijících v České republice, který se dokonale přizpůsobil k životu na kmenech stromů adaptacemi morfologickými (zbarvení, délka končetin) i etologickými. Síť této plachetnatky se skládá pouze z několika horizontálně a vertikálně napnutých vláken., Drapetisca socialis from the family Linyphiidae is a spider species with great morphological (coloration, long legs) and ethological (web reduction) adaptations to life on smooth tree trunks. This spider attaches an unusual reduced web vertically to tree trunks., and Ondřej Machač.
Permanent plastid-nuclear complexes (PNCs) exist in tobacco cells from their mitosis up to programmed cell death (PCD). PNCs in senescing cells of tobacco leaves were typical by enclosure of peroxisomes and mitochondria among chloroplasts which were in contact with nucleus. Such a complex position provides simultaneous interaction of these organelles and direct regulation of metabolism and PCD avoiding the cytosol. and T. Selga, M. Selga, A. Ozoliņa.
V článku je popsáno neobvyklé hemisynantropní chování plcha lesního (Dryomys nitedula) v domě v Oderských vrších nedaleko Bílovce (okres Nový Jičín). Zjištěný jedinec si ve dnech 14.-16. srpna vybudoval hnízdo v květináči s dračincem (Dracaena sp.), které bylo tvořeno suchými listy rostliny a ozdobnými krajkami., This article describes an unusual hemisynanthropic behaviour of one individual of the Forest Dormouse (Dryomys nitedula) observed at a house in the Oderské vrchy mountains near Bílovec (Nový Jičín district). On August 14-16, this dormouse built a nest in a flowerpot containing a Dracaena plant out of its dry leaves and also decorative lace., and Patrik Molitor.
V článku se zaměřujeme zejména na aktuální taxonomii, vybrané ekologické novinky a představíme druhy ploštěnek, které lze v České republice nalézt. Text je doplněn jednoduchými určovacími tabulemi pro všechny naše druhy, včetně jednoho skoro zapomenutého endemita., In this article we focus on current taxonomy and ecological behaviour, and we introduce the flatworm species that are found in the Czech Republic. The text is supplemented by simple drawings allowing determination of all Czech species, including a nearly forgotten endemic., and Marie Reslová, Ondřej Simon.
It is known that chromosomes occupy non-random positions in the cell nucleus. However, it is not clear to what extent their nuclear positions, together with their neighborhood, are conserved in daughter cells. To address specific aspects of this problem, we used the model of the chromosomes carrying ribosomal genes that are organized in clusters termed Nucleolus Organizer Regions (NORs). We compared the association of chosen NOR-bearing chromosomes (NOR-chromosomes) with nucleoli, as well as the numbers of nucleoli, in the pairs of daughter cells, and established how frequently the daughter cells had equal numbers of the homologs of certain NOR-chromosomes associated with individual nucleoli. The daughter cells typically had different numbers of nucleoli. At the same time, using immuno-FISH with probes for chromosomes 14 and 15 in HeLa cells, we found that the cell pairs with identical combinations appeared significantly more frequently than predicted by the random model. Thus, although the total number of chromosomes associated with nucleoli is variable, our data indicate that the position of the NOR-bearing chromosomes in relation to nucleoli is partly conserved through mitosis., M. Kalmárová, E. Smirnov, L. Kováčik, A. Popov, I. Raška., and Obsahuje bibliografii a bibliografické odkazy
The aim of our study was to answer whether any positive correlation exists between K+ uptake and salt tolerance in wheat. We carried out a sand-culture experiment with salt-tolerant, DK961 (ST), and salt-sensitive, JN17 (SS), wheat cultivars, where photosynthesis, the K+/Na+ ratio, growth, and the biomass yield were examined. The seeds were exposed for four weeks to six NaCl concentrations (50, 100, 150, 200, 250, and 300 mM), which were embodied in the Hoagland solution. Salinity-induced decrease of K+ or increase in the Na+ content was much smaller in ST than that in SS. The reductions in the light-saturated photosynthetic rate (P Nmax) and chlorophyll content caused by salinity were smaller in the ST compared to SS. Stomatal conductance decreased in both cultivars under saline conditions; nevertheless, it was lower in SS than in ST. The antioxidative capacity was higher in ST than that in SS under saline conditions. Significant positive correlations were observed in both cultivars between K+ contents and P Nmax/biomass yields. We suggest that higher-affinity K+ uptake might play a key role in higher salt tolerance and it might be a reliable indicator for breeding new species of salt-tolerant wheat., D. Cheng, G. Wu, Y. Zheng., and Obsahuje seznam literatury