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3372. Cadmium and zinc induced chlorosis in Indian mustard [Brassica juncea (L.) Czern] involves preferential loss of chlorophyll b
- Creator:
- Ebbs, S. and Uchil, S.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- heavy metals
- Language:
- Multiple languages
- Description:
- Plants of Indian mustard (Brassica juncea) were treated with either 50 µM Cd, 250 µM Zn, or 25 µM Cd+125 µM Zn and the progression of chlorosis in the mature leaves monitored. As relative chlorophyll (Chl) contents in the mature leaves decreased to 75, 50, and 25 % relative to controls, both mature and young leaves were harvested and the Chl pools extracted. The metal treatments caused a greater loss of Chl b than Chl a. As mature leaves underwent progressive chlorosis, the young leaves displayed a characteristic over-greening, due largely to increased content of Chl b. However, as the young leaves began to experience chlorosis, a greater loss of Chl b was also observed. Thus during metal induced chlorosis, there is a preferential turnover of the Chl b pool in mature and young leaves. and S. Ebbs, S. Uchil.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3373. Cadmium chloride toxicity revisited: effect on certain andrological, endocrinological and biochemical parameters of adult male rabbits
- Creator:
- Sajjad, S., Malik, H., Farooq, U., Rashid, F., Nasim, H., Tariq, S., and Rehman, S.
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, kadmium, kortizol, cadmium, cortisol, endocrine disruptors, prolactin, 14, and 612
- Language:
- English
- Description:
- The present study was devised to assess the effects of cadmium chloride (CdCl2) administration on certain andrological, endocrinological and biochemical alterations in adult male rabbits (n=24). The animals were assigned to control (n=8) and experimental (n=16) group. Experimental group was orally administered with 1.5 mg/kg body weight of CdCl2. The trials were carried out for a total of 5 weeks and blood sampling was carried out on weekly basis. A gradual decrease was noticed for body weight in the experimental group from week 1 to 5, being significantly lower in week 4 and 5 (P<0.05). A similar decremented trend was noticed for serum testosterone level being significantly lower in experimental group in week 4 and 5 (P<0.001). Significantly lower values were noticed for prolactin in experimental group in week 4 and 5 (P<0.05), than in the control. On the contrary, serum cortisol level showed a gradual increase in experimental group, from week 1 to 5, being significantly higher in week 4 and 5 (P<0.05). Regarding the biochemical attributes, all the parameters under study revealed a gradually ascending trend. Statistical significance was, however, achieved in varying weeks and at varying levels. The total protein and albumin were significantly higher in week 4 and 5 (P<0.01); alanine aminotransferase in week 2 (P<0.01), 3 (P<0.001), 4 (P<0.01) and 5 (P<0.001); aspartate aminotransferase in week 1, 2, 3, 4 and 5 (P<0.01); and alkaline phosphatase in week 1, 2 (P<0.01), 3, 4 and 5 (P<0.0001), respectively. Overall mortality rate in experimental group was 68.75 (11/16). In a nutshell, Cd exposure results in adverse effects on all physiological parameters of body and may lead to lethal consequences., S. Sajjad, H. Malik, U. Farooq, F. Rashid, H. Nasim, S. Tariq, S. Rehman., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3374. Cadmium induced ultrastructural changes in shoot apical meristem of Elodea canadensis Rich.
- Creator:
- Stoyanova, D. and Tchakalova, E.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- plastid, plastoglobuli, starch, and thylakoid
- Language:
- Multiple languages
- Description:
- The ultrastructure of cells of ground meristem in the peripheral zone of shoot apical meristem in Elodea canadensis Rich. was studied after treatment with cadmium in concentrations 0.5, 1, 2, 3, 5, and 6 g(Cd2+) m-3. After 5 d treatment, changes in the structural organisation of the plastid apparatus were found, namely in proplastid, amyloplast, and amoeboid stages of plastid development. and D. Stoyanova, E. Tchakalova.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3375. Cadmium is acutely toxic for murine hepatocytes: effects on intracellular free Ca2+ homeostasis
- Creator:
- Wang, S. S., Chen, L., and Xia, S. K.
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Biochemie. Molekulární biologie. Biofyzika, biochemie, toxicita, kadmium, jaterní buňky, homeostáza, biochemistry, toxicity, cadmium, liver cells, homeostasis, kalciový iont, calcium ion, 2, and 577
- Language:
- English
- Description:
- We studied cadmium toxicity in murine hepatocytes in vitro. Cadmium effects on intracellular free Ca2+ concentration ([Ca2+]i) were assayed, using a laser scanning confocal microscope with a fluorescent probe, Fluo-3/AM. The results showed that administration of cadmium chloride (CdCl2, 5, 10, 25 μM) resulted in a dose-dependent decrease of hepatocyte viability and an elevated aspartate aminotransfe rase (AST) activity in the culture medium (p<0.05 for 25 μM CdCl2 vs. control). Significant increases of lactate dehydrogenase (LDH) activities in 10 and 25 μM CdCl2-exposed groups were observed (p<0.05 and p<0.01, respectively). A greatly decreased albumin content and a more malondialdehyde (MDA) formation also occurred after CdCl2 treatment. The Ca2+ concentrations in the culture medium of CdCl2-exposed hepatocytes were significantly decreased, while [Ca2+]i appeared to be significantly elevated (p<0.05 or p<0.01 vs. control). We found that in Ca2+-containing hydroxyethyl piperazine ethanesulfonic acid-buffered salt solution (HBSS) only, CdCl2 elicited [Ca2+]i increases, which comprised an initially slow ascent and a strong elevated phase. However, in Ca2+-containing HBSS with addition of 2-aminoethoxydiphenyl borane (2-APB), CdCl2 caused a mild [Ca 2+] i elevation in the absence of an initial rise phase. Removal of extracellular Ca2+ showed that CdCl2 induced an initially slow [Ca2+]i rise alone without being followed by a markedly elevated phase, but in a Ca2+-free HBSS with addition of 2-APB, CdCl2 failed to elicit the [Ca2+]i elevation. These results suggest that abnormal Ca2+ homeostasis due to cadmium may be an important mechanism of the development of the toxic effect in murine hepatocytes. [Ca2+]i elevation in acutely cadmium-exposed hepatocytes is closely related to the extracellular Ca2+ entry and an excessive release of Ca2+ from intracellular stores., S. S. Wang, L. Chen, S. K. Xia., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3376. Cadmium stress in Dongying wild soybean seedlings: growth, Cd accumulation, and photosynthesis
- Creator:
- Liu, L., Shang, Y. K., Li, L., Chen, Y. H., Qin, Z. Z., Zhou, L. J., Yuan, M., Ding, C. B., Liu, J., Huang, Y., Yang, R. W., Zhou, Y. H., and Liao, J. Q.
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- pigmenty, pigments, atomic absorption spectrophotometry, Glycine soja, growth analysis, 2, and 581
- Language:
- Multiple languages
- Description:
- In order to understand better Cd resistance in soybean, Dongying wild soybean treated with different Cd concentrations were evaluated. The biomass, chlorophyll (Chl) content, leaf color, Chl a fluorescence parameters, photosynthesis parameters, and Cd contents were determined. Our results showed that when Cd concentration was ≤ 2 kg m-3, no significant decrease in biomass, photosynthetic parameters, and maximal photochemical efficiency of PSII was observed. This indicated that Dongying wild soybean resisted Cd toxic effects under such conditions. In addition, atomic absorption experiment results demonstrated that when Cd concentration was ≤ 0.5 kg m-3, the accumulation of Cd in wild soybean was lower in roots than that in shoots, while the accumulation of Cd was higher in roots than that in shoots when Cd concentration was ≥ 1 kg m-3. Therefore, Dongying wild soybean showed a certain resistance to Cd and could serve as a valuable germplasm resource for improving the breeding of Cd-resistant soybean., L. Liu, Y. K. Shang, L. Li, Y. H. Chen, Z. Z. Qin, L. J. Zhou, M. Yuan, C. B. Ding, J. Liu, Y. Huang, R. W. Yang, Y. H. Zhou, J. Q. Liao., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3377. Caemmerer, S. von: Biochemical models of leaf photosynthesis
- Creator:
- Čatský, J.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- biochemical models
- Language:
- Multiple languages
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3378. Caenorhabditis elegans perilipin is implicated in cold-induced lipolysis and inhibits autophagy in early embryos
- Creator:
- Kaššák, F., Chughtai, A. A., Kaššák, S., and Kostrouchová, M.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- Perilipin, lipolysis, hormone-sensitive lipase, autophagy, lipophagy, and Caenorhabditis elegans
- Language:
- English
- Description:
- Animals use neutral lipids, particularly triacylglycerols (TAGs), to store energy. TAGs are universally organized into dynamic cytoplasmic structures called lipid droplets (LDs). In mammals TAG breakdown is catalysed by lipases, such as hormonesensitive lipase (HSL). LD membrane-resident proteins called perilipins (PLINs) regulate some of these lipases. The model organism Caenorhabditis elegans has a single known PLIN homologue and orthologues of most lipases including HSL. HOSL-1 (the HSL orthologue in C. elegans) is responsible for production of cryoprotective glycerol in cold conditions, in addition to its role in fasting-induced lipolysis. We employed this model of cold exposure to study the role of PLIN-1 in the regulation of HOSL-1. Our results suggest that both HOSL-1 and PLIN-1 are required for cold tolerance and for lipid breakdown in cold. However, the loss of PLIN-1 partially rescued the phenotype of hosl-1 null mutants exposed to cold, suggesting the presence of an alternative pathway generating glycerol via lipolysis. In early embryos, PLIN-1 knock-out results in accumulation of lipids and formation of cytoplasmic clusters of autophagic marker LGG-1, supporting the role of autophagy as an alternative lipolytic pathway in C. elegans, as is the case in mammals.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
3379. Cairaella henrii gen. n., sp. n., a parasite of Norops trachyderma (Polychrotidae), and Ophiotaenia nicoleae sp. n. (Eucestoda: Proteocephalidea), a parasite of Thecadactylus rapicauda (Gekkonidae), in Ecuador
- Creator:
- Coquille, Sandrine C. and de Chambrier, Alain
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- Eucestoda, Proteocephalidea, Cairaella henrii, Ophiotaenia nicoleae, new genus, new species, morphology, and Squamata
- Language:
- English
- Description:
- Cairaella henrii gen. n., sp. n. (Proteocephalidea: Proteocephalinae) is described from the intestine of Norops trachyderma (Cope) (Polychrotidae) from San Pablo de Kantesiya in Ecuador. The new genus differs from the 12 other known genera of the Proteocephalinae (and all other proteocephalidean genera) by the presence of a dense network of osmoregulatory canals situated in the cortex and by the morphology of the scolex which is flattened dorsoventrally, with elongated deeply embedded suckers possessing a well-developed circular musculature situated in the anterolateral region, and by eggs with a three-layered embryophore possessing small outgrowths on its external surface. Ophiotaenia nicoleae sp. n. is described from the intestine of Thecadactylus rapicauda (Houttuyn) (Gekkonidae) from San Pablo de Kantesiya in Ecuador. This new species is characterised by the testes arranged in two fields, numbering 142-204, the cirrus-sac length representing 21-33% of proglottis width, the genital pore situated in the middle of the proglottis or slightly anteriorly, and the ovary width representing 68-88% of proglottis width. It differs from 20 of 27 Ophiotaenia species parasitic in New World reptiles by the presence of an apical organ and from the remaining species by one to several other morphological characters, such as the number of testes, diameter and shape of the scolex, position of ventral and dorsal osmoregulatory canals, or the presence of a vaginal sphincter. Both taxa represent the first record of proteocephalidean tapeworms in polychrotid and gekkonid lizards, respectively.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3380. Calcitriol and Punica granatum extract concomitantly attenuate cardiomyopathy of diabetic mother rats and their neonates via activation of Raf/MEK/ERK signalling and mitigation of apoptotic pathways.
- Creator:
- El-Mansi, A. A. and Al-Kahtani, M. A.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- calcitriol, Punica granatum, diabetes, cardiomyopathy, MAPK pathway, and apoptosis
- Language:
- English
- Description:
- We investigated the detrimental effects of diabetes on myocardium of pregestational streptozo-tocin (STZ)-diabetic mother rats and their neonates via evaluations of oxidative redox, inflammatory and apoptotic pathways, also aiming to characterize whether calcitriol and/or pomegranate peel extract confer myocardial protection in hyperglycaemic dams and their foetuses via modulation of the Raf/MEK/ERK cascade. Sixty Sprague-Dawley female rats were randomized into five groups (N = 12): control, diabetic, diabetic treated with calcitriol and/or pomegranate peel extract (PPE), and mated with non-diabetic healthy males. After confirmation of pregnancy, treatments were kept until gestational day (E-18). Serum and cardiac tissues of mothers and foetuses were collected and processed for bio-chemical, histopathological, and molecular assess-ments., We observed that, compared to the control, diabetic mothers showed dramatically increased hyperglycaemia and hyperlipidaemia associated with decreased myocardial functions and disrupted maternal performance. Also, diabetic mothers and their neonates exhibited elevated levels of myocardial injury (troponin I, endothelin 1, creatine kinase-MB, lactate dehydrogenase), with increased pro-inflammatory cytokines (interleukin 1, interleukin 1β, transforming growth factor β) and oxidative redox. Concurrently, the MAPK pathway was significantly down-regulated with increased myocardial apoptotic activity. Furthermore, mRNA expression of angio-genic and fibrotic markers was significantly increased. Paradoxically, calcitriol and/or pomegranate peel extract alleviated these diabetic myocardial insults and normalized the aforementioned assayed parameters. Our findings hypothesized that calcitriol and/or pomegranate peel extract exerted cardioameliorative impacts due to their unique anti-oxidative and anti-inflammatory properties, and thus may be a promising treatment that directly targets the secondary myocardial complications of diabetes in dams and their offspring., and Corresponding author: Ahmed Elmansi
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public