The aim of this study was to evaluate myofibrillar creatine kinase (EC 2.7.3.2) activity on the background of the effect of substrate channeling by myosin ATPase and to compare it with creatine kinase (CK) activity of whole skinned fibers. In order to assess CK activity, skinned fibers were prepared from the rat psoas major muscles defined by light microscopy. The activity in permeabilized fibers after treatment with saponin, Triton X-100 and Ca2+-free medium reached 2.80, 6.97 and 3.32 m mol ATP min-1 mg-1 protein, respectively, when a coupled enzyme assay system with external hexokinase and glucose-6-phosphate dehydrogenase was used. Transmission electron microscopy (TEM) revealed a possible interference among activities of sarcolemmal, sarcoplasmic, myofibrillar and mitochondrial CK from persisting structures. For evaluation of the myofibrillar CK itself, a pure myofibrillar fraction was prepared. Fraction purity was confirmed by TEM and by enzymatic assays for marker enzymes. Two procedures, i.e. the coupled enzyme assay and the evaluation of phosphocreatine (Pcr) concentration before and after the CK reaction, were used for measurement of CK activity in this fraction. The procedures resulted in 3.2 nmol ATP min-1 mg-1 protein and 7.6 nmol PCr min-1 mg-1 protein, respectively. These alternative approaches revealed a discrepancy between the reacting portions of Pcr by more than 50 % , which provides information about the size of the effect, generally described as substrate channeling., M. Gregor, J. Mejsnar, A. Janovská, J. Žurmanová, O. Benada, B. Mejsnarová., and Obsahuje bibliografii
Th e paper aims at identifying, explaining and illustrating the aff ordances of “creative nonfi ction” as a style of writing social science. Th e fi rst part introduces creative nonfi ction as a method of writing which brings together empirical material and fi ction. In the second part, based on illustrations from my ethnographic research of European “crisis reporters,” written in the form of a novel about a fi ctional journalist, but also based on a review of existing social science research that employs a creative method of writing, I identify several main aff ordances of creative nonfi ction in socialscientifi c research. In particular, I argue that creative nonfi ction allows scientists to illustrate their fi ndings, to express them in an allegorical way, to organize data into a narrative, to let their pieces of research act in the social world, and to permeate research accounts with self-refl exive moments. I also discuss some apparent negative aff ordances: challenges that creative nonfi ction poses to readers and to the institutionalized academic discourse. Finally, I suggest that writing about sociological problems in the style of creative nonfi ction can help to produce more engaging and engaged texts, and I discuss the ethical implications of the approach. and Článek identifi kuje, vysvětluje a ilustruje afordance „kreativní nonfi kce“ jako stylu psaní o sociální vědě. V první části představuji kreativní nonfi kci jako metodu psaní, která kombinuje empirický materiál a imaginaci. Ve druhé části na základě ukázek z vlastního etnografi ckého výzkumu evropských “krizových reportérů”, psaného formou románu o fi ktivním novináři, a také na základě jiných sociálněvědných výzkumů, které používají kreativní metody psaní, identifi kuji několik hlavních afordancí kreativní nonfi kce v sociálněvědním výzkumu. Kreativní nonfi kce zejména umožňuje vědkyním a vědcům jinak a někdy lépe ilustrovat jejich zjištění, vyjádřit tato zjištění alegoricky, organizovat data a vytvářet narativ, podpořit efektivitu výzkumů v sociálním světě nebo také proložit výzkumný narativ seberefl exivními prvky. Text ale diskutuje i zjevné negativní afordance, tedy výzvy, které potenciálně plynou ze setkání kreativní nonfi kce se čtenářstvem a s institucionalizovaným akademickým diskurzem. Nakonec diskutuji některé etické implikace představeného přístupu a tvrdím, že psát o sociologických problémech ve stylu kreativní nonfi kce může pomoct vytvářet texty, které budou angažované i poutavé.
The purpose of this paper is to present a new paradigm and an innovative technology for thinking about the future. The concept of time synchronization is introduced as a technology to improve individual competency for balancing the continuous construciton of reinterpreted pasts, presents and futures in order to cope with the aceleration of change, complexity, and uncertainty. This new paradigm is driven by recognition of three factors: 1. Humans are both conservative and novelty generating. 2. Novelty is a key factor of life and humans address novelty through pattern-evolvign creativity. 3. Reality is defined through the unique ability of humans to anticipate and define experience in terms of pattern and category. This article asserts that rapidly expanding human pluarity and novelty require new models concerning relationships of past, present, and future. Such models should adequately address the rapidly changing and more complex conditions in which they are constructed and deconstructed, including the expanding opportunities that accompany them. and Arthur M. Harkins, George H. Kubik, John Moravec.
The acoustic emission (AE) test of step-loaded samples of coal with different moisture contents was conducted using an AG-X250KN rock servo test system and an AE21C AE-monitoring system to study the influence of moisture content on the creep characteristics of coal. The results show that the moisture content influences the creep characteristics of coal samples, in that the greater the moisture content, the greater the creep deformation of the coal and the lower the creep threshold, creep strength, and creep coefficient values. The improved Burgers model can better describe the mechanical properties of creep in coal. In the transient creep stage, the coal micropores and fractures are gradually expanded, and AE activity gradually increased. In the stable creep stage, the creep deformation rate of coal tended to be stable, while new fractures and AE events and energy were all decreased. In the accelerated creep stage, a large number of fractures were generated in the coal, which gradually developed into unstable macrofractures. The stored energy was released rapidly in a relatively short time span, and the number and energy of AE events reached their maximum value. Therefore, the AE characteristics of coal reflect the evolutionary process of damage during creep.
This study describes Crenosoma brasiliense (Nematoda, Metastrongyloidea), a new species parasitic in bronchi and bronchioles of Galictis cuja (Molina) (Carnivora, Mustelidae) from Brazil. This species differs from other 11 species of Crenosoma by having a cuticular projection at the distal end of the spicules, forming a prominent blade at the tip of the spicule, a vulval cuticular appendage with a triangular shape and prominent vulval lips. There are no previous records of species of Metastrongyloidea in G. cuja or species of Crenosoma in South America. Therefore, the new species represents the first host record and first geographical record of species of Crenosoma in South America.