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31282. Protective effect of S-adenosylmethionine against galactosamine-induced injury of rat hepatocytes in primary culture
- Creator:
- Kučera, O., Červinková, Z., Lotková, H., Křiváková, P. , Roušar, T., Mužáková , V. , Héžová, R., Kanďár , R. , and Rudolf , E.
- Type:
- article, model:article, and TEXT
- Subject:
- S-adenosylmethionine, Galactosamine, and Hepatocytes in vitro
- Language:
- English
- Description:
- The protective effect of S-adenosylmethionine (SAMe) on D-galactosamine (GalN)-induced damage to rat hepatocytes was tested in primary cultures. SAMe at concentrations of 50 and 1000 mg/l significantly reduced lactate dehydrogenase release from cells injured by 40 mM GalN after 24 h of incubation. There were no significant changes in urea production after 24 h among tested groups, including control hepatocytes. Exposure of hepatocytes to GalN leads to 3.5-fold decrease in urea synthesis after 48 h in comparison with control cell cultures. Addition of the highest dose of SAMe (1000 mg/l) into the culture media attenuated this decrease by 180 %. None of the tested doses of SAMe (5, 25, 50 and 1000 mg/l) affected considerably the reduced activity of mitochondrial dehydrogenases. The content of reduced and oxidized glutathione in GalN-exposed cells was diminished to 1.5 % and 16 %, respectively, of the control values after 24 h. Using only the highest concentration SAMe increased significantly these contents. SAMe had no effect on dramatically decreased albumin synthesis. These findings indicate beneficial effect of SAMe, especially of the highest concentration, on GalN-induced toxicity to rat hepatocytes in primary culture. This action of SAMe seems to be associated with reduction of plasma membrane damage and increased synthesis of glutathione.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
31283. Protective effect of vitamin E on brain ischaemia during ontogenesis
- Creator:
- Trojan, S.
- Type:
- article, model:article, and TEXT
- Subject:
- vilamin E, brain ischaemia, positive radial acceleration lOxg, and ontogenesis
- Language:
- English
- Description:
- Vitamin E in a dose of 150 mg and 300 mg/kg of body weight, administered i.p., has a protective effect - in the course of the whole ontogenesis - against stagnant hypoxia induced in the laboratory rat by positive radial acceleration + lOxg. The favourable influence of vitamin E is relatively greater In older animals.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
31284. Protective Effects of Melatonin on Myocardial Ischemia-Reperfusion Induced Infarct Size and Oxidative Changes
- Creator:
- Sahna, E., Parlakpinar, H., Turkoz, Y., and Acet, A.
- Type:
- article, model:article, and TEXT
- Subject:
- Melatonin, Reperfusion injury, Heart, Infarct size, Glutathione, and Malondialdehyde
- Language:
- English
- Description:
- Free radicals, calcium overloading and loss of membrane phospholipids play an important role in the development of ischemia/reperfusion (I/R) injury. Melatonin is a well-known antioxidant and free radical scavenger. Melatonin may also reduce the intracellular calcium overloading and inhibit lipid peroxidation. This study was designed to investigate the effects of melatonin on the I/R-induced cardiac infarct size in an in vivo rat model. We also investigated glutathione (GSH) levels, an antioxidant the levels of which are influenced by oxidative stress, and malondialdehyde (MDA) levels, which is an index of lipid peroxidation. To produce cardiac damage, the left main coronary artery was occluded for 30 min, followed by 120 min reperfusion, in anesthetized rats. Melatonin (10 mg/kg) or vehicle was given 10 min before ischemia via the jugular vein. Infarct size, expressed as the percentage of the risk zone, was found significantly greater in I/R group than in the melatonin-treated I/R group. MDA levels were significantly higher, but GSH levels were lower in the I/R group than in the control group. Melatonin significantly reduced the MDA values and increased the GSH levels. These results suggest that oxidative stress contributes to myocardial I/R injury and melatonin administration exerts a mitigating effect on infarct size. Furthermore, the results indicated that melatonin improves the antioxidant capacity of the heart and attenuates the degree of lipid peroxidation after I/R.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
31285. Protective Effects of Topical Alpha-Tocopherol Acetate on UVB Irradiation in Guinea Pigs: Importance of Free Radicals
- Creator:
- Saral, Y., Uyar, B., Ahmet Ayar, Naziroglu, M., and Yilmaz, S.
- Format:
- print, bez média, and svazek
- Type:
- article, studie, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Alpha-tocopherol, Antioxidants, Guinea pig, Lipid peroxide, Skin, Ultraviolet B, 14, and 612
- Language:
- English
- Description:
- Reactive oxygen species can be generated by daily exposure of the skin to ultraviolet light and may cause some subchronic and chronic skin disorders. The aim of this study was to investigate a possible preventive role of a-tocopherol acetate (ATA) on ultraviolet B (UVB) induced peroxidation by assessing lipid peroxide (LPO) levels and activity of reactive oxygen scavenging enzymes including glutathione peroxidase and superoxide dismutase (SOD) in guinea pigs. ATA was topically applied to the skin for three weeks before a single dose of 0.9 J/cm2 UVB irradiation on the skin and lipid peroxide levels and antioxidants in plasma, skin and liver and erythrocytes were determined after decapitation. Topical application of ATA prevented the UVB irradiation-induced reduction of scavenging enzyme activities in skin and erythrocytes. In conclusion, we suggest that topical applications of ATA before UVB irradiation is effective in protecting the skin from unwanted effects of UVB irradiation., Y. Saral, B. Uyar, A. Ayar, M. Naziroglu, S. Yilmaz., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
31286. Protective function of ceruloplasmin and its action on erythrocytes in men with prostate tumors
- Creator:
- Kotrikadze, N., Zibzibadze, M., Ramishvili, L., Alibegashvili., M., Chigogidze, T., and Managadze, L.
- Format:
- braille, electronic resource, remote, and elektronický zdroj
- Type:
- model:article, article, Text, statistics, and TEXT
- Subject:
- nádory prostaty--farmakoterapie, ceruloplasmin--farmakologie, erytrocyty--účinky léků, biochemická analýza krve, statistika jako téma, staří, lidé, mužské pohlaví, and lidé středního věku
- Language:
- English
- Description:
- Purpose: To investigate the protective action of Ceruloplasmin (Cp) on the lysis of erythrocytes in men with prostate tumors. Material and Methods: The blood erythrocytes of the patients with benign hyperplasia of prostate (BHP) and prostate adenocarcinoma (CaP) were studied. Patients at the age 60-75 with early stages of a cancer have been investigated. The control group was consisted of apparently healthy males with the compatible age. n=15 for each group. The clinical stage of the disease was diagnosed by means of rectal, histological and echographic examination of the prostate gland. Photometric methods were applied to register lysis dynamics in order to test out the protective action of Cp. Results: The nonspecific protective function of Cp preparation on BHP and CaP erythrocytes as well as on the control group erythrocytes was revealed. CaP erythrocytes have shown more sensitivity to the lysis provocative factor than BHP and the control group erythrocytes, that was presumably attributed to the structural and functional changes of the erythrocytes developed in the presence of malignant tumor., N. Kotrikadze, M. Zibzibadze, L. Ramishvili, M. Alibegashvili., T. Chigogidze, L. Managadze, and Literatura
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
31287. Protective influence of vitamin E on antioxidant defense system in the blood of rats treated with cadmium
- Creator:
- Ognjanović, B. I., Pavlović, S. Z., Maletić, S. D., Žikić, R. V., Štajn, A. Š., Radojičić, R. M., Saičić, Z. S., and Petrović, V. M.
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, vitamin E, kadmium, krev, cadmium, blood, lipid peroxides, antioxidant defense system, 14, and 612
- Language:
- English
- Description:
- The effects of acute exposure to cadmium (Cd) on the blood antioxidant defense system, lipid peroxide concentration and hematological parameters, as well as the possible protective role of vitamin E were studied. Male Wistar albino rats (3 months old) were treated with cadmium (0.4 mg Cd/kg b.m., i.p., 24 h before the experiment) or with vitamin E + Cd (20 IU Vit E/kg b.m., i.m., 48 h + 0.4 mg Cd/kg b.m., i.p., 24 h before the experiment). The hematological parameters were assessed: red blood cell counts, hematocrit value and hemoglobin concentration were significantly decreased in the blood of Cd-treated rats. Intoxication with cadmium was also followed by significantly increased lipid peroxide concentrations. We also observed increased activity of antioxidant defense enzymes: copper zinc containing superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase as well as concentrations of non-enzymatic components of antioxidant defense system: reduced glutathione, vitamin C and vitamin E. Pretreatment with vitamin E exhibited a protective role on the toxic effects of cadmium on the hematological values, lipid peroxide concentration as well as on enzymatic and non-enzymatic components of antioxidant defense system., B. I. Ognjanović, S. Z. Pavlović, S. D. Maletić, R. V. Žikić, A. Š. Štajn, R. M. Radojičić, Z. S. Saičić, V. M. Petrović., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
31288. Protectorate tourism in the German Third Reich Network /
- Creator:
- Štemberk, Jan,
- Type:
- text and studie
- Language:
- English
- Rights:
- unknown
31289. Protein biotoxins of military significance
- Creator:
- Patočka, Jiří
- Type:
- model:article and TEXT
- Language:
- English
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
31290. Protein interactions with quaternized chitosan / heprain multilayers
- Creator:
- Kumorek, M., Dana Kubies, and Riedel, T.
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, chitosan, bílkoviny, proteins, heparin, basic fibroblasts growth factor, layer-by-layer, 14, and 612
- Language:
- English
- Description:
- Understanding the behavior of single proteins at the polyelectrolyte multilayer film/solution interface is of prime importance for the designing of bio-functionalized surface coatings. In the present paper, we study the adsorption of the model proteins, albumin and lysozyme, as well as basic fibroblast growth factor (FGF-2) on a polysaccharide multilayer film composed of quaternized chitosan and heparin. Several analytical methods were used to describe the formation of the polysaccharide film and its interactions with the proteins. Both albumin and lysozyme adsorbed on quaternized chitosan/heparin films, however this process strongly depended on the terminating polysaccharide. Protein adsorption was driven mainly by electrostatic interactions between protein and the terminal layer of the film. The effective binding of FGF-2 by the heparinterminated film suggested that other interactions could also contribute to the adsorption process. We believe that this FGF-2- presenting polysaccharide film may serve as a biofunctional surface coating for biologically-related applications., M. Kumorek, D. Kubies, T. Riedel., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public