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42. A new species of Spirorchis MacCallum, 1918 (Digenea: Schistosomatoidea) and Spirorchis cf. scripta from chicken turtle, Deirochelys reticularia (Emydidae), with an emendation and molecular phylogeny of Spirorchis
- Creator:
- Roberts, Jackson R, Orélis-Ribeiro, Raphael, Halanych, Kenneth M, Arias, Cova R, and Bullard, Stephen A
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- parazitologie, parasitology, taxonomy, Spirorchiidae, Spirhapalum, 28S phylogeny, Baracktrema, 2, and 59
- Language:
- English
- Description:
- Chicken turtles, Deirochelys reticularia (Latreille in Sonnini et Latreille) (Testudines: Emydidae) from Alabama, USA were infected by Spirorchis collinsi Roberts et Bullard sp. n. and Spirorchis cf. scripta. The new species is most easily differentiated from its congeners by the combination of having caeca that extend far beyond the genitalia, intercaecal genitalia positioned in the middle portion of the body, a testicular column that nearly abuts the caecal bifurcation, a cirrus sac positioned between the testes and ovary, a massive Mehlis' gland, an elongate, longitudinal metraterm that extends anteriad beyond the level of the ovary, a pre-ovarian genital pore, and a prominent, intercaecal Manter's organ. The specimens of S. cf. scripta differed from the holotype and published descriptions of Spirorchis scripta Stunkard, 1923 by several subtle morphological features, perhaps comprising intraspecific variation, but collectively warranted a detailed description herein. Based on examinations of the aforementioned specimens plus the holotype, paratypes and vouchers of morphologically-similar congeners, Spirorchis MacCallum, 1918 is emended to include the presence of oral sucker spines, a pharynx, lateral oesophageal diverticula ('plicate organ') and a median oesophageal diverticulum ('oeseophageal pouch'). Phylogenetic analysis of the nuclear large subunit rDNA (28S) recovered S. collinsi sister to Spirorchis picta Stunkard, 1923, > 99% similarity between S. cf. scripta and S. scripta, and a monophyletic Spirorchis MacCallum, 1918. No blood fluke infection has been reported previously from these drainages, Alabama, or this turtle species. This is the first new species of Spirorchis to be described from North America in 26 years., Jackson R. Roberts, Raphael Orélis-Ribeiro, Kenneth M. Halanych, Cova R. Arias, Stephen A. Bullard., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
43. A new species of Wallinia Pearse, 1920 (Digenea: Allocreadiidae) in Astyanax mexicanus (Characidae) from Mexico revealed by morphology and sequences of the 28S ribosomal RNA gene
- Creator:
- Pérez-Ponce de León, Gerardo, Razo-Mendivil, Ulises, Mendoza-Garfias, Berenit, Rubio-Godoy, Miguel, and Choudhury, Anindo
- Format:
- electronic, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- DNA (nukleová kyselina), parazitičtí červi, sladkovodní ryby, deoxyribonucleic acid, helminth parasites, freshwater fishes, Severní Amerika, North America, taxonomy, 2, and 59
- Language:
- English
- Description:
- Wallinia mexicana sp. n. is described from the Mexican tetra, Astyanax mexicanus (De Filippi) (Characidae Weitzman), from two localities in northern Mexico. The new species can be distinguished from the two congeneric species, described from small-bodied characids in South and Central America, mainly by the posterior extent of the vitelline follicles (halfway between the posterior testis and the end of the caeca), by having a larger oesophagus, testes that are always oblique, and eye-spot remnants. The distinct status of the new species was confirmed by molecular data (28S rRNA gene sequences). Phylogenetic analysis suggests the new species is the sister species of W. chavarriae Choudhury, Hartvigsen et Brooks, 2002 described from characids in northwestern Costa Rica. Additionally, genetic divergence between these congeners reached 3.3%, a value higher than that observed for closely related species pairs of allocreadiids for that molecular marker. Based on these new findings, recently published records of this new species as Magnivitellinum simplex Kloss, 1966 and Creptotrematina aguirrepequenoi Jiménez-Guzmán, 1973 in Astyanax mexicanus from Durango and San Luis Potosi states, respectively, are corrected., Gerardo Pérez-Ponce de León, Ulises Razo-Mendivil, Berenit Mendoza-Garfias, Miguel Rubio-Godoy, Anindo Choudhury., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
44. A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species
- Creator:
- Gerba, Madougou, Loiseau, Anne, Benoit, Laure, and Gauthier, Nathalie
- Format:
- print, počítač, and online zdroj
- Type:
- article, články, journal articles, model:article, and TEXT
- Subject:
- Zoologie, blanokřídlí, lumčíkovití, populační genetika, Hymenoptera, Braconidae, population genetics, Habrobracon hebetor, Bracon spp., biological control, parasitic wasp, microsatellite, gene flow, 2, and 59
- Language:
- English
- Description:
- Combining a biotin-enrichment protocol and 454GS-FLX titanium pyrosequencing technology, we characterised 22 polymorphic microsatellite loci from the parasitic wasp, Habrobracon hebetor (Say) (Hymenoptera: Braconidae), a cosmopolitan species commonly used in biological control against a wide range of both major lepidopterous pests of stored products and field crops in different parts of the world. Three multiplex PCR sets were optimised and characterised across 46 H. hebetor specimens from two samples collected from millet fields in Niger. Two to 11 alleles were found per locus and observed heterozygosity ranged from 0.289 to 0.826. Polymorphism was detected in both samples with a similar level of observed heterozygosity (0.482 vs. 0.502) and number of alleles (4.1 vs. 3.6). Deviation from Hardy-Weinberg equilibrium was detected at the same five loci in both samples and five or seven more loci in each sample but was not associated with heterozygote deficiencies. Even though evidence for linkage disequilibrium was found between a few alleles, these new loci segregated independently. The variability of the 22 loci will enable estimates of genetic diversity and structure patterns, as well as gene flow between H. hebetor populations at different spatial scales. Cross-species amplifications were successful among the six Bracon spp. tested and nine loci will be particularly appropriate for population genetic studies in B. brevicornis., Madougou Garba, Anne Loiseau, Laure Benoit, Nathalie Gauthier., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
45. A newly developed SGB buffer greatly enhances energy transfer efficiency from phycobilisomes to photosystem II in cyanobacteria in vitro
- Creator:
- Chen, L. P., Wang, Q. X., and Ma, W. M.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- botanika, botany, energy transfer efficiency, photosystem II, phycobilisome, SGB buffer, Synechocystis sp. PCC 6803, thylakoid membrane, 2, and 58
- Language:
- Multiple languages
- Description:
- The transfer of light energy from phycobilisomes (PBS) to photosystem II (PSII) reaction centers is vital for photosynthesis in cyanobacteria and red algae. To investigate the relationship between PBS and PSII and to optimize the energy transfer efficiency from PBS to PSII, isolation of the PBS-PSII supercomplex is necessary. SPC (sucrose/phosphate/citrate) is a conventional buffer for isolating PBS-PSII supercomplex in cyanobacteria. However, the energy transfer occurring in the supercomplex is poor. Here, we developed a new buffer named SGB by adding 1M glycinebetaine and additional sucrose to SPC buffer. Compared to SPC, the newly developed SGB buffer greatly enhanced the associated populations of PBS with thylakoid membranes and PSII and further improved the energy transfer efficiency from PBS to PSII reaction centers in cyanobacteria in vitro. Therefore, we conclude that SGB is an excellent buffer for isolating the PBS-PSII supercomplex and for enhancing the energy transfer efficiency from PBS to PSII reaction centers in cyanobacteria in vitro., L. P. Chen, Q. X. Wang, W. M. Ma., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
46. A non-invasive method for detecting the metabolic stress response in rodents: characterization and disruption of the circadian corticosterone rhythm
- Creator:
- Thanos, P. K., Cavigelli, S. A., Michaelides, M., Olvet, D. M., Patel, U., Diep, M. N., and Volkow, N. D.
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Biochemie. Molekulární biologie. Biofyzika, fyziologie, stres (fyziologie), metabolity, cirkadiánní rytmy, physiology, stress (physiology), metabolites, circadian rhythms, corticosterone, fecal steroid metabolites, 2, and 577
- Language:
- English
- Description:
- Plasma corticosterone (CORT) measures are a common procedure to detect stress responses in rodents. However, the procedure is invasive and can influence CORT levels, making it less than ideal for monitoring CORT circadian rhythms. In the current paper, we examined the applicability of a non-invasive fecal CORT metabolite measure to assess the circadian rhythm. We compared fecal CORT metabolite levels to circulating CORT levels, and analyzed change in the fecal circadian rhythm following an acute stressor (i.e. blood sampling by tail veil catheter). Fecal and blood samples were collected from male adolescent rats and analyzed for CORT metabolites and circulating CORT respectively. Fecal samples were collected hourly for 24 h before and after blood draw. On average, peak fecal CORT metabolite values occurred 7-9 h after the plasma CORT peak and time-matched fecal CORT values were well correlated with plasma CORT. As a result of the rapid blood draw, fecal production and CORT levels were altered the next day. These results indicate fecal CORT metabolite measures can be used to assess conditions that disrupt the circadian CORT rhythm, and provide a method to measure long-term changes in CORT production. This can benefit research that requires long-term glucocorticoid assessment (e.g. stress mechanisms underlying health)., P. K. Thanos ... [et al.]., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
47. A novel association between Aphaenogaster subterranea (Hymenoptera: Formicidae) and the nymphs of Reptalus panzeri (Hemiptera: Cixiidae)
- Creator:
- Lörinczi, Gábor
- Type:
- article, články, model:article, and TEXT
- Subject:
- Zoologie, zoologie, entomologie, Hymenoptera, Formicidae, Aphaenogaster subterranea, Hemiptera, Cixiidae, Reptaluls panzeri, ant-hemipteran, association, insect-insect relationship, myrmecophily, Fraxinus ornus, ash, 2, and 59
- Language:
- English
- Description:
- Gábor Lörinczi.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
48. A novel endosymbiont-containing trypanosomatid Phytomonas borealis sp. n. from the predatory bug Picromerus bidens (Heteroptera: Pentatomidae)
- Creator:
- Ganyukova, Anna I, Frolov, Alexander O, Malysheva, Marina N, Spodareva, Viktoria V, Vyacheslav Yurchenko, and Kostygov, Alexei Yu
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- dravé ryby, predatory fishes, new trypanosomatid species, prokaryotic endosymbionts, non-specific infection, 2, and 59
- Language:
- English
- Description:
- Here we describe the new trypanosomatid, Phytomonas borealis sp. n., from the midgut of the spiked shieldbugs, Picromerus bidens (Linnaeus), collected in two locations, Novgorod and Pskov Oblasts of Russia. The phylogenetic analyses, based on the 18S rRNA gene, demonstrated that this flagellate is a sister species to the secondary monoxenous Phytomonas nordicus Frolov et Malysheva, 1993, which was concurrently documented in the same host species in Pskov Oblast. Unlike P. nordicus, which can complete its development (including exit to haemolymph and penetration into salivary glands) in Picromerus bidens, the new species did not form any extraintestinal stages in the host. It also did not produce endomastigotes, indispensable for transmission in other Phytomonas spp. These observations, along with the fact that P. bidens overwinters at the egg stage, led us to the conclusion that the examined infections with P. borealis were non-specific. Strikingly, the flagellates from the Novgorod population contained prokaryotic endosymbionts, whereas the parasites from the second locality were endosymbiont-free. This is a first case documenting presence of intracellular symbiotic bacteria in Phytomonas spp. We suggest that this novel endosymbiotic association arose very recently and did not become obligate yet. Further investigation of P. borealis and its intracellular bacteria may shed light on the origin and early evolution of endosymbiosis in trypanosomatids., Anna I. Ganyukova, Alexander O. Frolov, Marina N. Malysheva, Viktoria V. Spodareva, Vyacheslav Yurchenko and Alexei Yu. Kostygov., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
49. A novel method for the estimation of soybean chlorophyll content using a smartphone and image analysis
- Creator:
- Rigon, J. P. G., Capuani, S., Fernandes, D. M., and Guimarães, T. M.
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- chlorofyl, karotenoidy, chlorophyll, carotenoids, mathematical models, fotosyntetické pigmenty, přenosná zařízení, quinoa, sezam, sójové boby, ricinový olej, tmavě zelený barevný index, vegetační index, smart phone camera, color model, photosynthetic pigments, portable equipment, sesame, soybean, castor oil, pod formation stage, Surinam cherry, dark green color index, vegetation index, 2, and 581
- Language:
- Multiple languages
- Description:
- The development of smartphones, specifically their cameras, and imaging technologies has enabled their use as sensors/measurement tools. Here we aimed to evaluate the applicability of a fast and noninvasive method for the estimation of total chlorophyll (Chl), Chl a, Chl b, and carotenoids (Car) content of soybean plants using a smartphone camera. Single leaf disc images were obtained using a smartphone camera. Subsequently, for the same leaf discs, a Chl meter was used to obtain the relative index of Chl and the photosynthetic pigments were then determined using a classic method. The RGB, HSB and CIELab color models were extracted from the smartphone images and correlated to Chl values obtained using a Chl meter and by a standard laboratory protocol. The smartphone camera was sensitive enough to capture successfully a broad range of Chl and Car contents seen in soybean leaves. Although there was a variation between color models, some of the proposed regressions (e.g., the S and b index from HSB and Lab color models and NRI [RGB model]) were very close to the Chl meter values. Based on our findings, smartphones can be used for rapid and accurate estimation of soybean and Car contents in soybean leaves., J. P. G. Rigon, S. Capuani, D. M. Fernandes, T. M. Guimarães., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
50. A novel myxozoan parasite of terrestrial mammals: description of Soricimyxum minuti sp. n. (Myxosporea) in pygmy shrew Sorex minutus from Hungary
- Creator:
- Székely, Csaba, Cech, Gábor, Atkinson, Stephen D, Kálmán Molnár, Egyed, László, and Gubányi, András
- Format:
- electronic, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- žlučové cesty, játra, biliary ducts, liver, Maďarsko, Hungary, Myxozoa, Soricimyxum spp., bile ducts, mammalian hosts, Sorex spp., 2, and 59
- Language:
- English
- Description:
- As part of a biodiversity study in northwestern Hungary, we conducted a parasitological survey of small mammals. In both common shrews (Sorex araneus Linnaeus) and pygmy shrews (Sorex minutus Linnaeus), we found myxospores of a species of Soricimyxum Prunescu, Prunescu, Pucek et Lom, 2007 (Myxosporea) and plasmodia in the bile ducts within the liver. Spores from both species of shrewswere morphologically and morphometrically indistinguishable, but differed in their SSU rRNA gene sequences by 3.3%. We identified spores and developmental stages from the common shrew as Soricimyxum fegati Prunescu, Prunescu, Pucek et Lom, 2007, based on morphometric data and DNA sequence similarity. Spores from the pygmy shrew were only 96.7% similar to S. fegati, hence we identified them as a novel myxosporean Soricimyxum minuti sp. n. This is only the second myxosporean parasite species described from mammals., Csaba Székely, Gábor Cech, Stephen D. Atkinson, Kálmán Molnár, László Egyed, András Gubányi., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public