Nitrogen-containing bisphosphonates were found to inhibit farnesyl diphosphate synthase - an essential enzyme in the cholesterol biosynthesis pathway, but their effect on cholesterol synthesis per se in the central nervous system (CNS) remains unknown. The aim of the present study was to examine possible influence of a representative agent alendronate on cholesterol synthesis rates in selected parts of rat CNS and on plasma cholesterol level. Two groups of rats were orally administered either alendronate (3 mg/kg b.w. ) or vehicle for 9 days. At the end of experiment, brain (basal ganglia, frontal cortex and hippocampus) and spinal cord were isolated and cholesterol synthesis was determined using the technique of deuterium incorporation from deuterated wa ter. In the alendronate group significant reductions of choleste rol synthesis rates were detected in frontal cortex, hippocampus and spinal cord (p<0.001). However, the experimental treatment did not produce a significant alteration in the levels of plasma cholesterol. In conclusion, this study brings the first experimental evidence of the inhibition of cholesterol biosynthesis with alendronate in central nervous system., Ľ. Cibičková, R. Hyšpler, N. Cibiček, E. Čermáková, V. Palička., and Obsahuje bibliografii
Práce podává přehled práce francouzského experimentálního psychologa Alfreda Bineta (1857–1911) v oblasti studia disociačních jevů. V úvodu jsou uvedeny základní profesní a biografické údaje a dále popis jeho díla v oblasti studia hypnózy, hysterie a obecně problematiky disociačních jevů. Pozornost je věnována hlavním dílům k této problematice, která jsou popisována chronologicky a v souvislosti s Binetovým odborným vývojem i širší situací v oboru psychologie. Práce představuje méně známé dílo francouzského psychologa přelomu 19. a 20., který se proslavil především studiem teorie a měření inteligence., The article reviews works of famous experimental psychologist Alfred Binet (1857-1911) in the area of dissociative phenomena studies. Introductory part of the article provides the basic professional and biographic information and further description of Binet’s work in the area of hypnosis, hysteria and generally area of dissociative phenomenon is given. Special attention is given to the main books written on these topics. The works are described in chronological order and in relation to Binet’s professional development. The article presents less known work of famous French psychologist of turn of 19th and 20th century, famous above all with the studies of intelligence., Radek Ptáček., and Obsahuje seznam literatury
The Tiebori Department of Phototrophic Microorganisms of the Institute of Microbiology received a donation from the Operational Programme, Research and Innovation for Development, for construction and operation of a new scientific research Centre of algal biotechnologies. The implementation phase (begun January 1, 2011) aims to develop new cultivation equipment and algal biomass processing practices for the production of biofuel, animal feeding, food supplements and for the isolation of valuable substances. The project will also focus on research in the area of photosynthesis, developing new measuring devices and educating students in these areas. and Ondřej Prášil a Petra Pfeiferová.
The consequences of epileptic seizures related to postictal inhibition in early postictal period include postictal analgesia. We studied this phenomenon over 96 h following flurothyl-induced seizures in adult male Wistar rats. Nociception of control (no seizure) and seizured groups were tested using the plantar and von Frey hair tests. We determined latency of forepaw and hind paw reactions using plantar tests and the number of von Frey hairs reactions. Shortly after seizures, longer plantar test latencies were seen relative to the control group. Before the seizures the plantar test reaction times were significantly shorter in forepaws than in hind paws. The effect disappeared post-seizure and surprisingly, it also disappeared at the corresponding time in controls; it reappeared after 48 h in the seizure group and after 24 h in controls. Differences in the von Frey hairs test occurred at 5 and 60 min post-seizure, however, these differences could not be explained by limb anatomy; although, different thermal and mechanical nociception mechanisms could be significant. The unexpected reactions in controls could be related to brief social and physical interactions between the two groups. and J. Mareš, R. Rokyta.