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2. Comparative study of mtDNA in species of the genus Adalia (Coleoptera: Cocinellidae) and origin of ancient mitochondrial haplotypes in the gene pool of Adalia bipunctata
- Creator:
- Zakharov, Ilya and Shaikevich, Elena
- Type:
- article, články, model:article, and TEXT
- Subject:
- Zoologie, zoologie, entomologie, Coleoptera, Cocinellidae, Adalia bipunctata, COI, ITS2, Rickettsia, 2, and 59
- Language:
- English
- Description:
- Fifteen different mitochondrial haplotypes of the mtDNA gene COI encoding cytochrome C oxidase subunit I were identified in the 127 individuals of Adalia bipunctata studied. Two mitochondrial haplotypes, H9 and H10, differed greatly from the others. The mitochondrial polymorphism in A. bipunctata is ancient, though its age remains to be evaluated. It is shown that mitochondrial haplotypes H9 and H10 and others coexisted in the original population of A. bipunctata before it spread throughout Eurasia from Western Europe to the Baikal Area, and before the differentiation of the subspecies A. bipunctata fasciatopunctata, which differs from the European form in its elytral pattern. In order to evaluate the possible origin of the ancient mitochondrial haplotypes in the gene pool of A. bipunctata sequences of the mtDNA gene COI and of the rRNA second internal transcribed spacer of the four species of Adalia: A. bipunctata, A. decempunctata, A. frigida and A. tetraspilota, were compared. It is suggested that infection with Rickettsia had an important role in the preservation of the mitochondrial haplotypes H9 and H10 during the evolution of Adalia., Ilya Zakharov, Elena Shaikevich., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
3. Dryophthorinae weevils (Coleoptera: Curculionidae) of the forest floor in Southeast Asia: Three-marker analysis reveals monophyly of Asian Stromboscerini and new identity of rediscovered Tasactes
- Creator:
- Grebennikov, Vasily V
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- nosatcovití, fylogeneze, Curculionid beetles, phylogeny, Čína, Nepál, Myanma, China, Nepal, Myanmar, Dryophthorinae, Tasactes, COI, ITS2, 28S, clogging taxonomy, 2, and 59
- Language:
- English
- Description:
- The nominal genus Tasactes Faust, 1894, consisting of two originally included nominal species from Myanmar, is rediscovered for the first time since being erected. Adult weevils herein assigned to the taxonomically re-defined Tasactes were abundant in forest floor litter at five localities in China (Yunnan and Sichuan), plus one specimen is available from Shaanxi and three from Nepal. Phylogenetic analysis of a 2,275 bp matrix concatenated from one mitochondrial (COI) and two nuclear markers (ITS2 and 28S) revealed that the monophyletic Tasactes consists of eight evolutionary significant terminal clades, either allopatric (three) or sympatric (two on Cang Shan in Yunnan and three on Mount Emei in Sichuan). The genus Tasactes is nested within the monophyletic Stromboscerini, while the tribe is sister to monophyletic Dryophthorus. The two morphological diagnostic characters of Tasactes, which are unique within the tribe, are the transversely truncated antennal club and conically projecting velvety apex of the club. So defined, Tasactes renders the genus Orthosinus paraphyletic. Considering the taxonomic neglect and uncertainties surrounding nominal Stromboscerini, all herein reported members of this tribe, including the Tasactes, are not assigned to Linnaean species. This paper illustrates the "clogging taxonomy" phenomenon, in which obscure historical names render taxonomic assignment of newly sampled specimens precarious. All the data used herein (localities, sequences, specimen images) are available online in public datasets dx.doi.org/10.5883/DS-TASACT1 and dx.doi.org/10.5883/DS-TASACT2., Vasily V. Grebennikov., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
4. First DNA analysis of pill scarabs (Coleoptera: Hybosoridae: Ceratocanthinae) reveals multiple paraphyly of Afrotropical Philharmostes
- Creator:
- Grebennikov, Vasily V.
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- Coleoptera, Hybosoridae, Ceratocanthinae, Ceratocanthini, DNA barcode, ITS2, 28S, phylogeny, forest litter, taxonomy, Philharmostes ballerioi sp. n., Afrotropical Region, and Eastern Arc Mountains
- Language:
- English
- Description:
- This paper is the first attempt to resolve relationships among the Ceratocanthinae: Ceratocanthini pill scarab beetles using DNA sequences. It is focused on the Philharmostes group of seven Afrotropical genera: Baloghianestes (3 spp.), Callophilharmostes (1 sp.), Carinophilharmostes (1 sp.), Chaetophilharmostes (1 sp.), Cryptophilharmostes (3 spp.), Petrovitzostes (1 sp.) and Philharmostes (31 spp.). A phylogenetic analysis of 46 terminals and alignment of 2,913 bp from one mitochondrial and two nuclear fragments corroborates monophyly of this group, but rejects that of Philharmostes, the largest genus. The latter is paraphyletic with respect to at least four other smaller genera and consists of at least three distantly related clades. One of them, formed by Philharmostes ballerioi sp. n. from the Tanzanian Nguru (the type locality) and Kaguru Mountains, is sister to the rest of the entire Philharmostes group. The nominal genus Philharmostes is, therefore, a waste-basket taxon for accommodating members of this group that lack the distinct characters of the smaller genera. Pending further research, the phylogenetically inadequate generic taxonomy of the Philharmostes group is not modified. Molecular clock analysis estimates separation of the mitochondrial lineages of two known populations of the new species at about 2.2 Ma, which corresponds with recurring shrinkage and expansion of African rainforest caused by climatic fluctuations during the Pleistocene. Adults of all nominal ingroup genera are illustrated along with male and female body parts of the new species. Diagnostic and/or synapomorphic morphological characters of the Philharmostes group of genera are revised. Habitus images and other supplementary information on all sequenced specimens are available online at dx.doi.org/10.5883/DS-VGDS001 and dx.doi.org/10.5883/DS-VGDS004.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
5. First molecular assessment of the interrelationships of cladorchiid digeneans (Digenea: Paramphistomoidea), parasites of Neotropical fishes, including descriptions of three new species and new host and geographical records
- Creator:
- Pantoja, Camila, Tomáš Scholz, Luque, José Luis, and Arlene Jones
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- motolice, vrubozubcovití, Trematoda, Cichlidae, Jižní Amerika, South America, diversity, taxonomy, new species, SEM, 28S rDNA, ITS2, cox1, Characiformes, Siluriformes, 2, and 59
- Language:
- English
- Description:
- a1_The first molecular assessment of phylogenetic relationships of cladorchiid digeneans (superfamily Paramphistomoidea Fischoeder, 1901) from freshwater fishes based on 28S rDNA, ITS2 and cox1 sequences reveals the subfamilies Dadayiinae Fukui, 1929 and Kalitrematinae Travassos, 1933 as non-monophyletic, whereas Dadaytrema Travassos, 1931 represented by three species is monophyletic. Fourteen species of cladorchiids were found in characiform, perciform and siluriform fishes in the Neotropical Region (Brazil and Peru), with numerous new host and geographical records. The first scanning electron micrographs of seven species are presented. Two new species of dadayiine and one new species of kalitrematine paramphistomes are described. Microrchis macrovarium sp. n. from Pimelodella cristata (Müller et Troschel) (type host), Tetranematichthys quadrifilis (Kner) and Pterodoras granulosus (Valenciennes) in Brazil and Peru differs from all three congeners in that the testes are directly tandem, not oblique, and that the ovary, between the caecal ends, is widely separated from the testes. Pronamphistoma philippei sp. n. from Heros sp. in Brazil is distinguished from the type and only species, Pronamphistoma cichlasomae Thatcher, 1992, by the absence of the anterior collar-like expansion present in the type species, the presence of extramural rather than intramural pharyngeal sacs, and the unusual development of the dorsal and ventral exterior circular muscle fibre series in the acetabulum., a2_Pseudocladorchis romani sp. n. from P. granulosus (type host), Brachyplatystoma vaillantii (Valenciennes), Calophysus macropterus (Lichtenstein), Megalodoras uranoscopus (Eigenmann et Eigenmann) and Oxydoras niger (Valenciennes) in Brazil and Peru, is most similar to Pseudocladorchis nephrodorchis Daday, 1907 but differs in the shape of the testes (irregular, versus reniform in the latter species) and the size of the ovary (as large as, or larger than, the testes in the new species). The generic diagnosis of Pronamphistoma Thatcher, 1992 is amended. Dadaytremoides parauchenipteri Lunaschi, 1989 is transferred to Doradamphistoma Thatcher, 1979 as D. parauchenipteri (Lunaschi, 1989) comb. n. based on morphological and molecular evidence., Camila Pantoja, Tomáš Scholz, José Luis Luque, Arlene Jones., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
6. Molecular approach for identification of mosquito species (Diptera: Culicidae) in Province of Alessandria, Piedmont, Italy
- Creator:
- Talbalaghi, Asghar and Shaikevich, Elena
- Type:
- article, články, model:article, and TEXT
- Subject:
- Zoologie, entomologie, Itálie severní, Italy, Northern, mosquitoes, Culicidae, Anopheles maculipennis, Ochlerotatus caspius, Culex pipiens, PCR-RFLP, ITS2, COI, 2, and 59
- Language:
- English
- Description:
- The aim of the present work was to identify cryptic species in the Anopheles maculipennis and Culex pipiens complexes and to study the genetic structure of the dominant mosquito species Ochlerotatus caspius (Diptera: Culicidae) in the Province of Alessandria close to the vast area untreated rice fields in Piedmont, NW Italy. With the help of PCR-RFLP analysis, four members of the Anopheles maculipennis complex were identified: A. messeae, A. maculipennis, A. sacharovi and A. atroparvus. Only C. pipiens f. molestus was identified in 11 habitats studied in Piedmont. Partial sequences of the cytochrome c oxidase subunit 1 (COI) mitochondrial gene and the second internal transcribed spacer (ITS2) of nuclear ribosomal RNA genes for Italian O. caspius are reported here for the first time. The results indicate that this species diverged from Iranian representatives of this species about one million years ago. The great diversity of mosquito species in Piedmont considerably increases the risk of vector-borne diseases. and Asghar TALBALAGHI, Elena SHAIKEVICH.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
7. Molecular identification of morphologically similar microcercous cercariae of two trematode families, Paragonimidae and Troglotrematidae, concurrently found in the same snail species of the subfamily Triculinae
- Creator:
- Doanh, Pham Ngoc, Van Hien, Hoang, and Dung, Bui Thi
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- morfologie (biologie), morphology (biology), Vietnam, ITS2, digenean larvae, microcercariae, 2, and 59
- Language:
- English
- Description:
- Microcercous cercariae possess a very short tail and are produced by digenean species of several families including medically important species, such as members of the genera Paragonimus Braun, 1899, Nanophyetus Chapin, 1927 and Troglotrema Odhner, 1914. During our survey of cercariae of Paragonimus spp. in Vietnam, we found microcercous cercariae from ten (0.29%) out of 3,400 snails of Triculinae gen. sp. 2. They were morphologically and molecularly analysed for species identification. The molecular analysis, based on ITS2 sequences, revealed two distinct species: four specimens were identical to Paragonimus proliferus Hsia et Chen, 1964 (Paragonimidae Dollfus, 1939), and the other six specimens were closest to members of the family Troglotrematidae Odhner, 1914 and were temporarily named Troglotrematidae gen. sp. Morphologically, cercariae of the two species found in this study are similar to each other in their gross characteristics but can be distinguished from one another by subtle morphological details. The cercaria of P. proliferus has an I-shaped excretory bladder and does not have mucous gland cells. In contrast, that of Troglotrematidae gen. sp. has a Y-shaped excretory bladder and mucous gland cells. Besides, the redia of P. proliferus is elongate with a short intestine and contains 5-6 cercariae whereas that of Troglotrematidae gen. sp. is more round with a longer intestine and harbours 3-4 cercariae. Our results have shown the importance of the shape of the excretory bladder and the presence/absence of mucous gland cells of the cercaria as well as the shape and size of the redia, and its intestinal length as valuable taxonomic characters of intramolluscan trematode larvae. In addition, the finding of similar microcercous cercariae of different species in the same snail species suggests that careful attention to morphological details is required in the differentiation of Paragonimus cercariae and those of closely related species., Pham Ngoc Doanh, Hoang Van Hien and Bui Thi Dung., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
8. Molecular studies of Anopheles culicifacies (Diptera: Culicidae) in Sri Lanka: Sibling species B and E show sequence identity at multiple loci
- Creator:
- Surendran, Sinnathamby Noble, Hawkes, Nicola, Steven, Andrew, Hemingway, Janet, and Ramasamy, Ranjan
- Type:
- article, model:article, and TEXT
- Subject:
- Anopheles culicifacies, species B, species E, Y-chromosome dimorphism, cytochrome oxidase II, ITS2, 28S rDNA, and guanylate cyclase
- Language:
- English
- Description:
- The anomaly that Anopheles culicifacies (Diptera: Culicidae) species B is a major vector of malaria in Sri Lanka, but a non-vector in India, has been noted for several years. In 1999, a Y chromosome dimorphism associated with Plasmodium vivax infectivity within the Indian A. culicifacies species B suggested that this was itself a complex of two sibling species, B and E. A recent cytogenetic analysis shows the sympatric presence of these sibling species in Sri Lanka, a situation similar to that reported from nearby Rameshwaram Island, India. Species E, with a submetacentric Y chromosome, is a more effective vector of P. vivax than species B with an acrocentric Y chromosome. Larval karyotyping, however, is time-consuming and labour-intensive. Recently, the development of a PCR-RFLP assay distinguishing species B and E of A. culicifacies from India, based on differences in one region of the cytochrome oxidase subunit II (COII) gene, was reported. Here we show that whilst this diagnostic approach reveals polymorphism in Sri Lankan A. culicifacies, this variation is not correlated with Y chromosome karyotype. Hence this assay will not be useful for distinguishing species B and E in Sri Lanka. Further, we found no difference between the sequences of Sri Lankan specimens in any of three other regions (ITS2, D3 region of 28S rDNA, and guanylate cyclase intron) often used for species discrimination.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
9. Morphological and molecular characteristics of adult worms of Gnathostoma Owen, 1836 (Nematoda) collected from domestic pigs in Dien Bien Province, northern Vietnam
- Creator:
- Tuyen, Nguyen Van, Lan, Nguyen Thi Kim, and Doanh, Pham Ngoc
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- morfologie (biologie), morphology (biology), Asie jihovýchodní, Asia, Southeastern, Gnathostomatidae, cox1, ITS2, 2, and 59
- Language:
- English
- Description:
- Gnathostomes are of interest because of their unique appearance and medical importance. Among 13 valid species of the genus Gnathostoma Owen, 1836 (Nematoda: Spirurida), two species, G. doloresi Tubangui, 1925 and G. hispidum Fedtschenko, 1872, are parasites of pigs but their larvae can infect humans to cause gnathostomiasis. In this study, we collected adults of Gnathostoma sp. from the stomach of domestic pigs (Sus scrofa domesticus Linnaeus) from Dien Bien Province, northern Vietnam. Morphologically, nematodes found here are most similar to G. doloresi with a slight difference in the spicules of males. In contrast, they are genetically distinct from G. doloresi and other species of Gnathostoma in both ITS2 and cox1 sequences. The findings of the present study suggest that specimens of Gnathostoma sp. found in Dien Bien are likely a new species and emphasise the need of further studies on the taxonomy and phylogenetic relationship of species of Gnathostoma. Special attention should also be paid to swine and human gnathostomiasis in Dien Bien Province, Vietnam and the neighbouring areas of China and Laos., Nguyen Van Tuyen, Nguyen Thi Kim Lan, Pham Ngoc Doanh., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
10. New type of xiphidiocercariae (Digenea: Microphalloidea) from South Vietnam
- Creator:
- Krupenko, Darya, Gonchar, Anna, Kremnev, Georgii, Efeykin, Boris, and Krapivin, Vladimir
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- Digenea, xiphidiocercariae, sporocysts, Xiphidiata, Microphalloidea, Pachypsolidae, 28S rDNA, ITS1, ITS2, and COI
- Language:
- English
- Description:
- We found unusual digenean intramolluscan stages, sporocysts and cercariae, in gastropods Sulcospira dautzenbergiana (Morelet) (Caenogastropoda: Pachychilidae) from Southern Vietnam and named them Cercaria cattieni 1. These cercariae have a stylet and thus belong to the Xiphidiata. However, such combination of characters as extremely large body size and I-shaped excretory bladder has not been found before in any other xiphidiocercariae. We obtained COI, ITS1, 5.8S + ITS2, and 28S rDNA sequences for C. cattieni 1. The latter allowed us to specify the phylogenetic position of the discovered cercariae: C. cattieni 1 falls within the superfamily Microphalloidea and is most closely grouped to Pachypsolus irroratus (Rudolphi, 1819) (Pachypsolidae), the sea turtle parasite. Information on the family Pachypsolidae is limited. Judging from the molecular phylogeny, C. cattieni 1 might be the larva of the Pachypsolidae, documented for the first time.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public