Glucagon and α-adrenergic-induced glycog enolysis is realized via the agonist/adenylyl cyclase/cAMP/protein kinase signaling pathway or via the activation of phosphorylase kinase by the mobilized calcium that supports the inhibition of glycogen synthase, respectively. The role of nitric oxide (NO) in this process has not been extensively studied. The present work was directed to the question whether NO is produced during glucagon-induced glycogenolysis in rat hepatocyte in a similar way like α-adrenoceptor stimulation. Glycogen-rich hepatocyte cultures were used. NO production (NO2-) was assessed under the influence of glucagon, dibutyryl cyclic AMP (db-cAMP), forskolin, the nitric oxide synthase (NOS) inhibitors Nω-nitro-L-arginine methyl ester (L-NAME) and aminoguanidine, and the NO donor S-nitroso-N-acetyl penicillamine (SNAP). Inducible NOS (iNOS) mRNA was examined by reverse transcription-polymerase chain reaction. Glycogenolysis was followed up by estimation of medium glucose levels. The amount of glucose and NO2- released by glycogen-rich hepatocytes was increased as a result of glucagon, db-cAMP, forskolin and SNAP treatments. iNOS gene expression was upregulated by glucagon. Glycogenolysis that occurs through glucagon receptor stimulation involves NO production downstream of transduction pathways through an isoform of NO synthase. The present and previous studies document possible involvement of NO signaling in glycogenolytic response to glucagon and adrenergic agonists in hepatocytes., H. Farghali, J. Hodis, N. Kutinová-Canová, P. Potměšil, E. Kmoníčková, Z. Zídek., and Obsahuje bibliografii a bibliografické odkazy
Rejstřík, Na předním přídeští je ex libris MUDr Julia Bílka, Vazba je zhotovena z lepenky pokryté ozdobným škrobovým papírem Vazba je v zachovalém stavu, vnitřní blok je mírně opotřebovaný, Digitalizace 2017 AiP Beroun, and Restaurování 2017 Jarmila Franková
přerušené číslování, ve zweiter Theil přeházené a zmatečné číslování, Rejstřík, Na předním přídeští je ex libris MUDr Julia Bílka, Vazba je zhotovena z lepenky pokryté ozdobným škrobovým papírem Vazba je v zachovalém stavu, vnitřní blok je mírně opotřebovaný, Digitalizace 2017 AiP Beroun, and Restaurování 2017 Jarmila Franková