Several pathophysiological mechanisms have been proposed in
the development of pregnancy complications, including
endothelial dysfunction, an inflammatory pathway and oxidative
stress. The aim of the present study was to evaluate the
correlation between proinflammatory cytokines TNF-α, IL-6 and
dual cytokine IL-10 in the mother’s peripheral blood and systolic
blood pressure, risk of preeclampsia and low birth weight in
gestational diabetes (GDM). We observed 40 women with GDM
divided into a gestational hypertension group (n=20) and
comparison group (n=20) with normal blood pressure. We found
a significant positive correlation between TNF-α; IL-6; IL-10
levels and systolic blood pressure (SBP) in the second trimester
(p<0.001; p<0.001; p<0.001); the third trimester (p<0.001;
p<0.001; p<0.05). We also proved correlations for diastolic blood
pressure (DBP) during the second; third trimester (p<0.001;
p<0.001; p<0.001); (p<0.001; p<0.001; p<0.0015). We
demonstrated a statistically significant positive association
between high TNF-α group and preeclampsia risk in the third
trimester (p=0.04). We also determined the negative correlation
in the second trimester between birth weight and TNF-α; IL-6,
IL-10 levels (p<0.05; p<0.001; p<0.001). To conclude, our data
highlight the importance of cytokines TNF-α, IL-6 and IL-10 in
blood pressure regulation. In addition, high levels of TNF-α have
been associated with increased risk of preeclampsia. We found
a significant negative correlation between levels of TNF-α, IL-6,
IL-10 and birth weight.
The aim of the study was to evaluate short-term heart rate variability (HRV) as an index of cardiac autonomic control in rats with lipopolysaccharide (LPS)-induced endotoxemia. Animals were injected intraperitoneally with LPS (100 μg/kg b.w.) and control group with an equivalent volume of saline. ECG recordings were done before (base) and 60, 120, 180, 240 and 300 min after LPS or saline administration. HRV magnitude was quantified by time and frequency-domain analysis (mean RR interval, SDRR, RMSSD, spectral powers in low (LF) and high frequency (HF) bands. Heart tissue homogenates and plasma were analyzed to determine interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α) and oxidative stress level (TBARS). Administration of lipopolysaccharide was followed by continuous rise in colonic body temperature compared to saline-treated controls. Endotoxemia in rats was accompanied by significant decrease in HRV spectral activity in high-frequency range at maximal body temperature (logHFpower: 1.2±0.5 vs. 1.9± 0.6 ms2, P<0.01). Increased IL-6 was found in heart tissue homogenates of LPS rats (8.0±0.6 vs. 26.4±4.8 pg/ml, (P<0.05). In conclusions, reduced HRV in HF band may indicate a decreased parasympathetic activity in LPS-induced endotoxemia as basic characteristics of altered cardiac control during response to endotoxemia., I. Zila, D. Mokra, J. Kopincova, M. Kolomaznik, M. Javorka, A. Calkovska., and Obsahuje bibliografii
The aim of the study was to assess the association between promoter polymorphism [A(-596)G] in interleukin-6 gene and office systolic and diastolic blood pressures, and the heart rate (HR) in apparently healthy Czech subjects. Furthermore, we evaluated the possible influence of gender, BMI and smoking on these supposed associations. An age-matched (40-50 years) and gender-matched (F/M=81/89) sample of apparently healthy Czech subjects (n=170, F/M=81/89) without hypertension, other cardiovascular diseases or diabetes was examined. The A(-596)G Il-6 gene polymorphism was detected by the PCR method. No differences in genotype distribution and/or allelic frequency was found between groups with lower systolic blood pressure (£ 122 mm Hg) and higher systolic blood pressure (> 122 mm Hg). Similarly, no differences in the IL-6 polymorphism were found between lower (£ 86 mm Hg) and higher (> 86 mm Hg) diastolic blood pressure groups. However, we proved a significant increase of genotypes AG+GG as well as the allele (-596)G in higher (>78 beats/min) heart rate group. The genotypes AG+GG represent significantly higher relative risk for higher HR frequency, especially in women. Among lean persons with a low heart rate frequency, fewer AG+GG genotypes were determined than among any other subjects. The genotypes AG+GG are more frequent in non-smoking persons with higher HR compared to non-smoking subjects with lower HR, especially in women. Gender, BMI and smoking substantially modify the distribution of A(-596)G Il-6 gene polymorphism in apparently healthy persons with lower or higher heart rate., A. Vašků, M. Souček, M. Goldbergová, J. Vácha., and Obsahuje bibliografii