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2. SWI/SNF2 proteins in germinal and early embryonic development

Creator:
Kokavec, Juraj, Vargová, Jarmila, Burda, Pavel, Vargová, Karin, Nečas, Emanuel, Stopka, Tomáš, Stopka, Pavel, and Dvořáková-Hortová, Kateřina
Type:
article and TEXT
Subject:
ISWI, Snf2h, Smarca5, Brg1 (Smarca 4), chromatin, epigenetic, stem cell, and primordial germ cell
Language:
English
Description:
Specific transcription factors participate in the decision making process that controls cell fate and differentiation. They function in the environment of chromatin and directly affect its structure and activity. This influence is especially apparent during the development regulation of gametes and in the course of the development of an early embryo. This review focuses on the role that Snf2h (Smarca 5) and Brg1 (Smarca 4), two factors belonging to the SWI/SNF2 family, play in the establishment of chromatin structure in germinal and early embryonic development.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/

3. T-lymphopoiesis is severely compromised in ubiquitin-green fluorescent protein transgenic mice

Creator:
Faltusová, K. , Báječný, M., Heizer, T., Páral, P., and Nečas, E.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
UBC-GFP mice, C57Bl, J mice, lymphopoiesis, myelopoiesis, haematopoiesis, stem cell, transplantation, green fluorescent protein, T cell, and B cell
Language:
English
Description:
Tagging cells of experimental organisms with genetic markers is commonly used in biomedical research. Insertion of artificial gene constructs can be highly beneficial for research as long as this tagging is functionally neutral and does not alter the tissue function. The transgenic UBC-GFP mouse has been recently found to be questionable in this respect, due to a latent stem cell defect compromising its lymphopoiesis and significantly influencing the results of competitive transplantation assays. In this study, we show that the stem cell defect present in UBC-GFP mice negatively affects T-lymphopoiesis significantly more than B-lymphopoiesis. The production of granulocytes is not negatively affected. The defect in T-lymphopoiesis causes a low total number of white blood cells in the peripheral blood of UBC-GFP mice which, together with the lower lymphoid/myeloid ratio in nucleated blood cells, is the only abnormal phenotype in untreated UBCGFP mice to have been found to date. The defective lymphopoiesis in UBC-GFP mice can be repaired by transplantation of congenic wild-type bone marrow cells, which then compensate for the insufficient production of T cells. Interestingly, the wild-type branch of haematopoiesis in chimaeric UBC-GFP/wild-type mice was more active in lymphopoiesis, and particularly towards production of T cells, compared to the lymphopoiesis in normal wild-type donors.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public