This reflection is inspired by a discussion among leading intermedialists organized by UNISC, Brazil, fall 2021. It included contextualization and conceptualization, i.e. setting the current research within the context of the discipline’s development and the synchronic study of culture, proposing new concepts or defending old ones. The key term ‘in-between’ expresses both a trend in art, and in the self-reflecting intermedial methodology. It becomes obvious that intermedial research opens up wide to analyzing issues of social importance. In our exposition, we assess the debated concepts in terms of their analytical and educational potential in literary and cultural studies, and relate the debate to the Czech environment.
The 48-hour "Aladin" forecast model can predict significant meteorological quantities in a middle scale area. Neural networks could try to replace some statistical techniques designed to adapt a global meteorological numerical forecast model for local conditions, described with real data surface observations. They succeed commonly a cut above problem solutions with a predefined testing data set, which provides bearing inputs for a trained model. Time-series predictions of the very complex and dynamic weather system are sophisticated and not any time faithful using simple neural network models entered only some few variables of their own next-time step estimations. Predicted values of a global meteorological forecast might instead enter a neural network locally trained model, for refine it. Differential polynomial neural network is a new neural network type developed by the author; it constructs and substitutes for an unknown general sum partial differential equation of a system description, with a total sum of fractional polynomial derivative terms. This type of non-linear regression is based on trained generalized data relations, decomposed into many partial derivative specifications. The characteristics of composite differential equation solutions of this indirect type of a function description can facilitate a much greater variety of model forms than is allowed using standard soft-computing methods. This adjective derivative model type is supposed to be able to solve much more complex problems than is usual using standard neural network techniques.
Cíl studie: Výsledek každé analýzy DNA je do značné míry závislý na typu biologického materiálu použitého pro extrakci nukleových kyselin. Cílem naší studie je porovnat vlastnosti DNA extraktů získaných dvěma různými izolačními postupy z buněk bukální sliznice, močového sedimentu, nehtů, vlasových kořínků a periferních krevních buněk. Materiál a metody: Biologické vzorky byly získány od 24 dobrovolníků s mediánem věku 31 let (25–54 let). Izolace DNA byla provedena fenol-chloroformovou extrakcí a pomocí separačních mikrokolonek (Qiagen). Eluční objem byl v obou případech 50 μl. Extrakty byly charakterizovány spektrofotometricky, fluorimetricky, elektroforeticky a z hlediska účinnosti následné PCR amplifikace. Výsledky: Nejvyšší koncentrace DNA obsahovaly extrakty z buněk periferní krve a bukální sliznice; nejnižší koncentrace DNA byly izolovány z vlasových kořínků. Všechny typy extraktů měly uspokojivou čistotu (mediány v rozmezí 1,7–1,9). Podíl nefragmentované DNA ve vzorcích získaných mikrokolonkovou metodou byl téměř dvojnásobný v porovnání s fenolovou extrakcí. Procentuální zastoupení nefragmentovaných molekul klesal podle biologického zdroje v následující řadě: krev (73,3 %) > bukální sliznice (63,5 %) > močový sediment (31,3 %) > nehty (25,3 %) > vlasy (20,4 %). Amplifikační účinnost u extraktů z periferní krve, bukálního stěru a močového sedimentu byla vyšší než u extraktů z nehtů a vlasů. Závěr: Všechny analyzované extrakty pocházející z krevních buněk, bukální sliznice, močového sedimentu, nehtů a vlasů poskytly dostatek DNA molekul k provedení molekulárně biologických vyšetření. Nejvhodnějšími materiály byly krevní buňky a buňky bukální sliznice. Z nich připravené extrakty měly nejvyšší koncentraci a čistotu DNA bez ohledu na použitou izolační metodu, nejnižší podíl degradované DNA a nejvyšší účinnost amplifikace krátkých i dlouhých amplikonů., Objective: Results of DNA testing depend in many cases on the type of biological material used for extraction of nucleic acids. The aim of the study is to compare properties of DNA extracts prepared using two different isolation procedures from buccal cells, urine sediment, nails, hair roots, and peripheral blood cells. Material and Methods: Biological material was collected from 24 volunteers at median age of 31 years (range 25–54 years). Phenol–chloroform extraction and spin microcolumn extraction method (Qiagen) were used for DNA isolation. In both the procedures, the elution volume was 50 μl. The extracts were characterized optically (UV spectrophotometric and fluorimetric analyses), electrophoretically, and by PCR amplification efficiency. Results: The highest DNA concentrations were found in extracts from peripheral blood and buccal cells; the lowest DNA concentrations were in hair extracts. All types of the extracts had acceptable purity (medians 1.7–1.9). The content of nonfragmented DNA molecules in the microcolumns extracts was almost twofold higher in comparison to the phenol ones. The percentages of non-fragmented DNA decreased as follows: blood (73.3 %) > buccal cells (63.5 %) > urine sediment (31.3 %) > nails (25.3 %) > hair roots (20.4 %). The amplification efficiency in the peripheral blood, buccal swab, and urine extracts was significantly higher than in the nail and hair extracts. Conclusion: All analyzed DNA extracts received from blood, buccal cells, urine sediment, nails, and hair roots provided a sufficient number of integral DNA molecules for following DNA testing. The best quality of DNA was found in extracts from blood and buccal cells (high concentrations and purity, low degree of fragmentation, and high efficiency of amplification for either short or long PCR amplicons)., Beránek M., Hegerová J., Drastíková M., and Literatura 25
In the wake of the national and political conflict in the Middle East, Arab-Jewish culture has undergone a process of marginalization and negligence, as well as a gradual descent into utter oblivion, owing to both Arab-Musim and Hebrew-Jewish-Zionist national and culural systems. Both sides, each with its own form of limited reasoning and particularistic considerations, have refused to accept the legitimacy of Arab-Jewish hybridism highlighting instead "pure" nationally, culturally, and religiously exclusive identities. The article explores the gradual demise of Arab-Jewish cultural hybridism, which, from a historical point of view, coexisted with Arab-Muslim and Arab-Christian hybridisms during some periods. Following a short era in the twentieth century during which Arab-Jewish culture flourished, especially in Egypt and Iraq, we are currently witnessing the demise of that culture. Consequently, Israeli-Arab Jews, or those seen as their offspring, currently have, or will have in the near future, three man cultural options. The first - the revival of active Jewish involvement in Arab canonical culture - is probably impossible. The second option is involvement in popular Israeli culture; this option is characterized by a strong longing for legitimacy - Jewish musicians and singers of Arab origin have accomplished a great deal in this field. The third option is participation in the activities of the canonical Hebrew culture.
The author examines the clientele of the Bratislava booksellers Anton Löwe and Philip Ulrich Mahler in the context of the Hungarian book trade from 1770 to 1800. By analysing the extant correspondence of Michal Institoris Mošovský, a protestant pastor in Bratislava, she was able to partially identify one segment of their customer base - protestant clergymen. For many years these members of the petty intelligentsia purchased from the Bratislava booksellers, in particular imported works by the German pietists and Enlightenment theologians. The author also investigated the social and geographical limits of the distribution process, some of the contact and distribution networks, and the identity of key figures., Petronela Križanová., and Obsahuje bibliografické odkazy
Známý kritik politiky Spojených států, lingvista a filozof Noam Chomsky v této publikaci rozvíjí své již dříve vyslovované teze a argumenty. Recenzent považuje jeho snahu zastávat se politicky marginalizovaných subjektů za sympatickou a leckteré postřehy za výstižné, vyčítá mu však jednostranné protiamerické zaujetí a zjednodušování problémů. V českém prostředí, kde jsou média naladěna naopak zřetelně proamericky, nicméně pokládá tuto knihu za užitečnou. and In the volume under review, the Czech translation of Hegemony or Survival: America’s Quest for Global Dominance (2003), Noam Chomsky, the well-known critic of US policy, a linguist, and philosopher, develops his earlier ideas and arguments. The reviewer considers Chomsky’s attempt to stand up for the politically marginalized to be an engaging one, and sees some of his observations as apposite. Nevertheless, the reviewer reproaches Chomsky for his one-sided anti-Americanism and oversimplification of the problems. All in all, however, the reviewer considers the work useful for Czechs, whose news media are clearly pro-American.