Unikátní vakcína pro léčbu infekčních onemocnění, historicky první zdokumentování vývoje zubu, které může pomoci v boji proti rakovině, revoluční objev organismu bez mitochondrií neboli „buněčných elektráren“ -i takové jsou aktuální výsledky vědeckých týmů z centra BIOCEV, které za účasti významných hostů zahájilo 16. června 2016 plný provoz., A unique vaccine to treat infectious diseases, tooth development documented the first time in history that might help fight cancer and the revolutionary discovery of an organism without mitochondria, referred to as “cell power plants”. These are some of the current results from the BIOCEV research teams. The centre’s full operation was officially launched on June 16, 2016 in the presence of Czech and foreign guests from different areas of science and politics. The Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec (BIOCEV) was established with substantial financial aid from the European Union. By 2020, 400 researchers and 200 master’s degree as well as postgraduate students are expected to work there. As of today, 56 research groups under five synergic research programmes are focused on obtaining more detailed understanding of organisms at the molecular level. Their results are oriented towards applied research and the development of new medical procedures to combat severe health problems., Isd., and Autor je podepsaný šifrou Isd.
Representatives of the Academy of Science of Czech Republic and Charles University have subscribed to the Founding Contract of the Biotechnological and Biomedical Center (BIOCEV) that will be situated in the vicinity of Vestec. and Luděk Svoboda.
ECM is composed of different collagenous and non-collagenous proteins. Collagen nanofibers play a dominant role in maintaining the biological and structural integrity of various tissues and organs, including bone, skin, tendon, blood vessels, and cartilage. Artificial collagen nanofibers are increasingly significant in numerous tissue engineering applications and seem to be ideal scaffolds for cell growth and proliferation. The modern tissue engineering task is to develop three-dimensional scaffolds of appropriate biological and biomechanical properties, at the same time mimicking the natural extracellular matrix and promoting tissue regeneration. Furthermore, it should be biodegradable, bioresorbable and non-inflammatory, should provide sufficient nutrient supply and have appropriate viscoelasticity and strength. Attributed to collagen features mentioned above, collagen fibers represent an obvious appropriate material for tissue engineering scaffolds. The aim of this minireview is, besides encapsulation of the basic biochemical and biophysical properties of collagen, to summarize the most promising modern methods and technologies for production of collagen nanofibers and scaffolds for artificial tissue development., L. Koláčná, J. Bakešová, F. Varga, E. Košťáková, L. Plánka, A. Nečas, D. Lukáš, E. Amler, V. Pelouch., and Obsahuje bibliografii
This work evaluates the myocardial protective potential of potassium cardioplegia on ischaemically arrested and reperfused hearts by two cardioplegic solutions: the University of Wisconsin solution (UW) and the standard crystalloid solution of St. Thomas’ Hospital (ST). Evaluation of myocardial preservation was based on creatine kinase and lactate releases and on high-energy phosphate preservation of isolated rabbit hearts after 4 hours’ hypothermic ischaemia. A morphometric ultrastructural evaluation of mitochondria in cardiomyocytes was also performed. The hearts of 24 rabbits were normothermally perfused with oxygenated Krebs-Henseleit solution for 30 min (Langendorff preparation), and the baseline contractile performance and biochemical parameters were evaluated. The hearts were then arrested and stored in the cardioplegic solutions (12 UW and 12 ST) at 4 °C for 4 hours. The hearts were then rewarmed and reperfused with oxygenated Krebs-Henseleit solution for further 30 min. At the end of reperfusion, creatine phosphate and high energy phosphates were higher with UW (p<0.05); creatine kinase release during reperfusion was significantly lower with UW both at 15 min (p<0.01) and at 30 min (p<0.05). Lactate release during the first 15 min of reperfusion was about doubled (p<0.05) with respect to controls in both groups; at 30 min this increase had almost vanished ( + 8 %) with UW but not with ST ( + 30 %). Ultrastructural morphometry did not show any significant difference at the level of mitochondria between the two treatments. The results indicate, for UW, an improved myocardial preservation associated with relative retention of high-energy phosphates and higher recovery of mechanical function, accelerated metabolic recovery and reduced stress of cell membranes.
The objective of this study was to investigate the early biological response in the olive flounder exposed to sub-lethal concentrations of waterborne phenanthrene (0.5, 1 or 2 μM). The fish were exposed for 4 weeks and we analyzed their enzymatic defense system, antioxidant and phase II enzyme activities, to evaluate the chronic exposure toxicity of phenanthrene. Waterborne phenanthrene affected antioxidant enzymes and glutathione-mediated detoxification as an enzyme defense system. Hepatic, gill and kidney glutathione reductase as well as glutathione S-transferase, and catalase activities were markedly elevated after two or four weeks of exposure. These enzyme activities of the olive flounder, Paralichthys olivaceus, seem to be a convenient approach for monitoring pollution in coastal areas against polycyclic aromatic hydrocarbon pollution including phenanthrene.
The aim of our study was to evaluate potential differences in the concentration of biochemical markers of endothelial dysfunction between essential hypertension, endocrine hypertension (pheochromocytoma, primary hyperaldosteronism) and control healthy group and to assess a potential relationship between these markers of endothelial dysfunction and
vasopressor substances overproduced in endocrine hypertension. We have investigated 21 patients with moderate essential hypertension, 29 patients with primary hyperaldosteronism, 24 subjects with pheochromocytoma and 26 healthy volunteers. Following parameters of endothelial dysfunction were measured, von Willebrand factor (vWf),
plasminogen activator (t-PA) and E-selectin (E-sel). Clinical blood pressure was measured according to the European Society of Hypertension recommendations. We found significantly higher levels of the von Willebrand factor in patients with essential hypertension in comparison with a control group (114±20 IU/dl vs 90±47 IU/dl; P=0.04) and patients with primary hyperaldosteronism (114±20 IU/dl vs 99±11 IU/dl; P=0.01). Patients with endocrine hypertension revealed increased levels of vWF compared to the control group, but these differences did not reach statistical significance. Levels of t-PA were increased in patients with pheochromocytoma in comparison with the control group (4.6±1.9 ng/ml vs 3.4±0.9 ng/ml; P=0.01) and with primary hyperaldosteronism (4.6±1.9 ng/ml vs 3.4±1.1 ng/ml; P<0.01). In case of E-selectin we found lower levels in patients with pheochromocytoma in comparison with other
groups, but they differed significantly only with primary hyperaldosteronism (40.2±15.0 ng/ml vs 51.3±23.0 ng/ml; P=0.05). Our study did not reveal any convincing evidence of differences in the levels of biochemical markers of endothelial dysfunction between essential and endocrine hypertension. No correlation between the biochemical markers of endothelial dysfunction and vasopressor substances activated in endocrine hypertension was found.