Ever since proteomics was proven to be capable of characterizing a large number of differences in both protein quality and quantity, it has been applied in various areas of biomedicine, ranging from the deciphering molecular pathogenesis of diseases to the characterization of novel drug targets and the discovery of potential diagnostic biomarkers. Indeed, the biomarker discovery in human plasma is clearly one of the areas with enormous potential. However, without proper planning and implementation of specific techniques, the efforts and expectations may very easily be hampered. Numerous earlier projects aimed at clinical proteomics, characterized by exaggerated enthusiasm, often underestimated some principal obstacles of plasma biomarker discovery. Consequently, ambiguous and insignificant results soon led to a more critical view in this field. In this article, we critically review the current state of proteomic approaches for biomarker discovery and validation, in order to provide basic information and guidelines for both clinicians and researchers. These need to be closely considered prior to initiation of a project aimed at plasma biomarker discovery. We also present a short overview of recent applications of clinical proteomics in biomarker discovery., V. Tambor ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
This paper presents the results of geophysical survey performed in the Pilawa River valley in the area of Middle Pomerania (Poland). The resistivity imaging method was applied. Resistivity profile measuring eight hundred metres allowed to investigate the geologic structure to the depth of 150 metres. The resistivity cross section shows the structure of Pleistocene sediments and the depth of Miocene - Pleistocene boundary. The significant lowering of the boundary is related to assumable ice-sheet margin range of Pomeranian phase of North Polish Glaciation. The lowering of the boundary may be a result of sediments compaction and the subglacial tunnel slope as well., Bogdan Żogała, Ryszard Dubiel, Józef Lewandowski, Waclaw M. Zuberek and Grzegorz Gąska., and Obsahuje bibliografické odkazy
Depletion of exploited mineral ore resources and their constant price increase have contributed to active interest in recognition and exploitation of new workable deposits. Exploration work which has been carried out in the vicinity of non-exploited ore areas such as Klucze, Zawiercie and Laski results in confirmation of Zn-Pb commercial deposits. In this region Zn-Pb ores occur in the Devonian and Triassic carbonate formation at a depth of 70 - 80 metres under the surface. Geological recognition is based on the analysis of data taken from bore-holes. To determine the mode of the ores occurrence and service conditions, it is necessity to work out a spatial image of overburden structure. For this reason resistivity measurements with highly distributed LUND Imaging System were introduced in the area of Zawiercie I. The studies were carried out according to three measuring protocols: Schlumberger, Wenner and dipole-dipole. The measurements were performed along three parallel six-hundred-metre profiles. The resistivity cross sections were elaborated using Res2D software. The results of the geophysical research were correlated with the data taken from bore-holes in order to testing the efficiency of applied geoelectrical methods. The study showed a significant diversification of geoelectric characteristics of the rockmass and thus it allowed to recognise accurately the overburden structure of the deposit and to locate precisely zones of faulting. In future, the applied geoelectrical methods are certainly to be used for localization ore bodies at a considarable depth range., Krzysztof Jochymczyk, Jerzy Cabala and Artur Poreba., and Obsahuje bibliografii
This research evaluates the potential benefits of the tightly combined processing of a global navigation satellite system together with the additional ranging observations from a satellite based augmentation system. In specific, the experiment presents performance of precise instantaneous single-frequency positioning based on European Galileo and EGNOS navigation systems. Due to currently low number of Galileo satellites, the test observational data were obtained with hardware GNSS signal simulator. All calculations were performed with in-house developed software - GINPOS. The results show that it is possible to obtain improvement in the accuracy and reliability of single-frequency precise positioning when including observations from SBAS systems. However, one must take in to account that at middle latitudes EGNOS satellites are observed at low elevations what results in higher atmospheric errors affecting its signals., Jacek Paziewski, Paweł Wielgosz and Marta Krukowksa., and Obsahuje bibliografické odkazy
Oil derivatives contamination of soils effects in changes of many physical properties of soil su ch as: electrical resistivity, conductivity and self potential (SP) as well. Thus the measurable a nomalies of these properties are possible to detect with geoelectrical measurements. SP geoelectrical measurements were carried out in the area of controlled oil spill. The oil was spilled into vadose zone of dry sands. The first underground water level was observed at the depth of 10 m. The measurements were carried out along 20 m long profile situated above 3 m long contaminated zone. The part of contaminated zone (1.5 m) had been subjected to bioremediation process with yeasts Yarrowia lipolytica since 4 months. In the border of contaminated zone and biodegradate done chemical gradient was developed and significant in crease of self potentials values can be observed. The measurements were carried out with potential gradient method. L und Imaging System equipment and non-polarizable electrodes (Cu/CuSO 4 ) were applied. Above contaminated and biodegraded zone distinct SP anomaly (10-20 mV) was measured. The anomaly correlates with results of EM, resistivity imaging and induced polarization investigations., Bogdan Żogała, Maciej J. Mendecki, Wacław M. Zuberek and Małgorzata Robak., and Obsahuje bibliografické odkazy
The paper presents the results of research related to the application of GNSS solutions in short observational periods in geodynamical investigations. Authors used the 3-hour solution appointed from hour-long interval of about 30 chosen stations on mountainous terrains from over 100 which were worked out. The main aim was to check the correctness of such solutions by the comparison with the daily ones. Some outliers in East component could testify, that tropospheric or ionospheric models used in the data adjustment are not sufficient for so short-time solutions. The second principal problem, which was considered in the present work is the ability to detect diurnal and sub-diurnal oscillations in changes of permanent stations’ coordinates. Results show unambiguously, that such oscillations appear in all analysed stations. In the paper there are examples of stations with dominant oscillations in different frequencies. The clear homogeneous in the frequencies was not found among any group of stations. It is therefore difficult to affirm, if their origin comes purely from the geodynamical phenomena., Andrzej Araszkiewicz, Janusz Bogusz, Mariusz Figurski and Karolina Szafranek., and Obsahuje bibliografii
This study deals with the application of the previously developed immobilized and perfused isolated hepatocytes as a cellular system for the study of representative phase 1 and phase II of biotransformation reactions. To illustrate phase I reactions, aminopyrine (0.17-4.25 mmol/1) and hexobarbital (0.2 mmol/1) were selected. For phase II reactions, glutathione transferase activity was evaluated by using l-chloro-2,4-dinitrobenzene (CDNB) as a substrate (0.125-2.0 mmol/1). Formaldehyde, that was formed from aminopyrine, increased steadily in the perfusion medium with time. The perfused hepatocytes eliminated hexobarbital at a much higher rate than the hepatocytes in suspension. At several time points the amount of CDNB-glutathione conjugate formed per one million hepatocytes in the bioreactor was almost twice the amount formed by the hepatocytes in suspension. The present data illustrate the successful application of the hepatocyte bioreactor in phase I and phase II of xenobiotic metabolism and indicate that the cells were metabolically more active than the cells in suspension.
It has been shown that, in addition to conventional contact electrode techniques, optical methods using fluorescent dyes can be successfully used for cardiac signal measurement. In this review, the physical and technical fundamentals of the method are described, as well as the properties of the most common systems for measuring action potentials and intracellular calcium concentration. Special attention is paid to summarizing limitations and trends in developing this method., M. Ronzhina, ... [et al.]., and Obsahuje seznam literatury
SSCP (single-strand conformation polymorphism) analyses of ubiquitin genes were used to investigate evolutionary relationships within the subgenus Orinocarabus of the genus Carabus. After SSCP electrophoresis of PCR-amplified ubiquitin copies, population-specific band patterns were obtained. Ubiquitin-SSCP-analyses of the six central European Orinocarabus species, including three subspecies and thirteen populations, resulted in a dendrogram that differed from that based on morphology. Phylogenetic analysis of mitochondrial DNA (mtDNA) did not support the SSCP dendrogram, but was in good accordance with the taxonomy based on morphological characters. The reason for the discrepancies seems to be evolutionary conservation of the ubiquitin genes. The time that elapsed since the evolution of the closely related Orinocarabus species is too short for concerted evolution of the ubiquitin genes.