Epileptic afterdischarges (ADs) elicited by electrical stimulation of sensorimotor cortical area were used as a model to study the role of neurotransmitter systems in cortical seizures in three age groups of developing rats. Drugs augmenting inhibition mediated by GABAA receptors were found to suppress ADs in all age groups, their activity was usually more marked in younger than in 25-day-old rat pups. Drugs potentiating GABAB receptors exhibit lower efficacy and more complicated developmental profile than GABAA-ergic drugs. Effects of an antagonist of GABAB receptor – marked prolongation of ADs in all three age groups – suggest an important role of GABAB receptors in arrest of cortical seizures. Drugs affecting glutamate receptors exhibit variable effects, usually better expressed in older animals than in 12-day-old ones. No specific role for ionotropic as well as metabotropic glutamate receptors could be predicted. Activation of adenosinergic inhibitory modulatory system also exhibited anticonvulsant action in the present model. All three neurotransmitter systems probably participate in mechanisms of generation, maintenance and arrest of cortical seizures., P. Mareš, H. Kubová., and Obsahuje bibliografii a bibliografické odkazy
Psychostimulants, as well as cannabinoids, have been shown to
significantly affect a great variety of behaviors in both humans
and laboratory animals. Our previous studies have repeatedly
demonstrated that the application of the vehicle for
psychostimulants, i.e. saline, to control groups, generated
different behavioral test results compared to absolute naïve
controls (i.e. without any injection). Therefore, our present study
has set three goals: (1) to evaluate the effect of three different
psychostimulant drugs, (2) to evaluate the effect of three doses
of delta 9-tetrahydrocannabinol (THC), and (3) to evaluate the
effect of saline and ethanol injections vs sham injections and no
injection on spontaneous behavior of adult male rats. The
LABORAS test (Metris B.V., Netherlands) was used to examine
spontaneous locomotor activity and exploratory behavior in an
unknown environment over 1 h. In Experiment 1,
psychostimulant drugs were tested: single subcutaneous (s.c.)
injections of amphetamine (5 mg/kg), cocaine (5 mg/kg), and
3,4-methylenedioxymethamphetamine (MDMA) (5 mg/kg) were
applied prior to testing. Control animals received the same
volume (1 ml/kg) of s.c. saline. In Experiment 2, the effect of
three doses of THC (1, 2, and 5 mg/kg, s.c.) were examined.
An s.c. injection of vehicle (ethanol) was used as a control. In
Experiment 3, injections of saline and ethanol were compared to
the group receiving a sham s.c. injection and to a group of
absolute “naïve” controls. Our results demonstrated that (1) all
psychostimulants increased locomotion time, distance traveled,
and speed while decreasing immobility time of adult male rats
relative to saline controls. The most prominent effect was
associated with MDMA; (2) The effect of THC was dosedependent and was most apparent within the first 10 min of the
LABORAS test. (3) With regard to the effect of injection: absolute
controls (without injection) compared to animals injected with
ethanol, saline, or sham-injected displayed reduced immobility
time, traveled longer distances, and had increased speed. In
conclusion, our data showed drug dependent behavioral changes
in adult male rats after application of psychostimulants and
cannabinoids. Our findings also suggest that not only drugs but
the actual single injection per se also affects the behavior of
laboratory animals in an unknown environment. This effect
seems to be associated with the acute stress associated
with the injection.
The quality of stored blood can be deteriorated by hemolysis caused by free radicals. The purpose of this study was to elucidate whether neutrophile leukocytes are the source of free radicals in stored blood as in hemodialyzed patients. Resuspensions with low (LL) or high (HL) leukocyte concentrations were prepared from samples of twenty healthy volunteers. The samples were incubated for 10 days at 4 °C and then for one day at 37 °C. Markers of hemolysis and free radical metabolism were examined before and after incubation in LL and HL samples. In spite of the difference of leukocytes counts in LL and HL resuspensions (p<0.0001), the pre-incubation values of all laboratory parameters were practically identical. In post-storage samples, superoxide dismutase and glutathione peroxidase activities did not differ in either group. Reduced glutathione in erythrocytes and extracellular antioxidant capacity were insignificantly lower in HL resuspensions, but the increase of malondialdehyde was much more pronounced in the HL samples (p<0.0001). The degree of hemolysis, expressed as the extracellular increase of potassium (p<0.001), hemoglobin (p<0.05) and lactate dehydrogenase (p<0.05), was higher in the HL samples. Our results support the hypothesis that leukocytes participate in free radical production in stored blood., J. Racek, R. Herynková, V. Holeček, J. Faltysová, I. Krejčová., and Obsahuje bibliografii
The carbon dioxide concentrating system in C4 photosynthesis allows high net photosynthetic rates (PN) at low internal carbon dioxide concentrations (Ci), permitting higher PN relative to stomatal conductance (gs) than in C3 plants. This relation would be reflected in the ratio of Ci to external ambient (Ca) carbon dioxide concentration, which is often given as 0.3 or 0.4 for C4 plants. For a Ca of 360 µmol mol-1 that would mean a Ci about 110-140 µmol mol-1. Our field observations made near midday on three weedy C4 species, Amaranthus retroflexus, Echinochloa crus-galli, and Setaria faberi, and the C4 crop Sorghum bicolor indicated mean values of Ci of 183-212 µ mol mol-1 at Ca = 360 µmol mol-1. Measurements in two other C4 crop species grown with three levels of N fertilizer indicated that while midday values of Ci at high photon flux were higher at limiting N, even at high nitrogen Ci averaged 212 and 196 µmol mol-1 for Amaranthus hypochondriacus and Zea mays, respectively. In these two crops midday Ci decreased with increasing leaf to air water vapor pressure difference. Averaged over all measurement days, the mean Ci across all C4 species was 198 µmol mol-1, for a Ci/Ca ratio of 0.55. Prior measurements on four herbaceous C3 species using the same instrument indicated an average Ci/Ca ratio of 0.69. Hence midday Ci values in C4 species under field conditions may often be considerably higher and more similar to those of C3 species than expected from measurements made on plants in controlled environments. Reducing gs in C4 crops at low water vapor pressure differences could potentially improve their water use efficiency without decreasing PN.
The article considers the nature of descriptive statements and the ontological status of descriptive constructs in linguistics, taking the example of a phoneme of English. It is argued that descriptive statements should be seen as expressions of the content of descriptive models or as hypotheses. Furthermore, it is argued that descriptive models and constructs in linguistics have a purely explanatory function in relation to speech events and without ontological commitment to corresponding entities in the real world., Článek pojednává o povaze popisných výroků a ontologickém stavu deskriptivních konstrukcí v lingvistice, přičemž vychází z příkladu fonému angličtiny. Argumentuje se tím, že popisná prohlášení by měla být chápána jako vyjádření obsahu popisných modelů nebo jako hypotézy. Dále, to je argumentoval, že popisné modely a konstrukce v lingvistice mají čistě vysvětlující funkci ve vztahu k událostem řeči a bez ontologického závazku k odpovídajícím entitám v reálném světě., and Paul Rastall
The paper sketches and defends two instances of the strategy Let N’s be whatever they have to be to explain our knowledge of them—one in which N’s are natural numbers and one in which N’s are propositions. The former, which makes heavy use of Hume’s principle and plural quantification, grounds our initial knowledge of number in (a) our identification of objects as falling under various types, (b) our ability to count (i.e. to pair memorized numerals with individuated objects of one’s attention), (c) our (initially perceptual) recognition of plural properties (e.g. being three in number), and (d) our predication of those properties of pluralities that possess them (even though no individuals in the pluralities do). Given this foundation, one can use Fregean techniques to non-paradoxically generate more extensive arithmetical knowledge. The second instance of my metaphysics-in-the-service-of-epistemology identifies propositions (i.e. semantic contents of some sentences, objects of the attitudes, and bearers of truth, falsity, necessity, contingency, and apriority) with certain kinds of purely representational cognitive acts, operations, or states. In addition to providing natural solutions to traditionally un-addressed epistemic problems involving linguistic cognition and language use, I argue that this metaphysical conception of propositions expands the solution spaces of many of the most recalcitrant and long-standing problems in natural-language semantics and the philosophy of language.
In this note, we prove that the countable compactness of {0, 1} R together with the Countable Axiom of Choice yields the existence of a nonmeasurable subset of . This is done by providing a family of nonmeasurable subsets of whose intersection with every non-negligible Lebesgue measurable set is still not Lebesgue measurable. We develop this note in three sections: the first presents the main result, the second recalls known results concerning non-Lebesgue measurability and its relations with the Axiom of Choice, the third is devoted to the proofs.
High temperature is a common constraint during anthesis and grain-filling stages of wheat leading to huge losses in yield. In order to understand the mechanism of heat tolerance during monocarpic senescence, the present study was carried out under field conditions by allowing two well characterized Triticum aestivum L. cultivars differing in heat tolerance, Hindi62 (heat-tolerant) and PBW343 (heat-susceptible), to suffer maximum heat stress under late sown conditions. Senescence was characterized by measuring photosynthesis related processes and endoproteolytic activity during non-stress environment (NSE) as well as heat-stress environment (HSE). There was a faster rate of senescence under HSE in both the genotypes. Hindi62, having pale yellow flag leaf with larger area, maintained cooler canopy under high temperatures than PBW343. The tolerance for high temperature in Hindi62 was clearly evident in terms of slower green-leaf area degradation, higher stomatal conductance, higher stability in maximum PSII efficiency, Rubisco activity and Rubisco content than PBW343. Both the genotypes exhibited lower endopeptidase activity under HSE as compared to NSE and this difference was more apparent in Hindi62. Serine proteases are the predominant proteases responsible for protein degradation under NSE as well as HSE. Flag leaf of both the genotypes exhibited high-molecular-mass endoproteases (78 kDa and 67 kDa) isoforms up to full grain maturity which were inhibited by specific serine protease inhibitor in both the environments. In conclusion, the heat-tolerant Hindi62 exhibited a slower rate of senescence than the heat-susceptible PBW343 during HSE, which may contribute towards heat stability. and S. Chauhan ... [et al.].
Když benevolence zraňuje ženy a favorizuje muže: účinky ambivalentního sexismu na aspirace na vůdcovství Literatura o ambivalentním sexismu ukázala, že benevolence vůči ženám může být dokonce škodlivější než otevřené nepřátelství, protože není jasně rozpoznatelná jako druh předsudku, a tak je obtížnější s ní bojovat. Podobně benevolence vůči mužům predikuje vnímanou oprávněnost genderové hierarchie. Účinek sexismu na mužské a ženské aspirace na vůdcovství však ještě nebyl zkoumán. Účastníci studie (N = 101) byli poučeni o sexismu (hostilním vs. benevolentním) vůči vlastnímu pohlaví. Výsledky ukázaly, že benevolence podporuje možnost udržení vůdcovské role u mužů, ale odrazuje ženy od vůdcovských pozic. Ženy dokonce považují benevolentní a hostilní postoje vůči ženám za podobné předsudky. Muži naopak vymezují hostilní sexismus vůči mužům jako více předsudečný než benevolentní postoje., Literature on ambivalent sexism has shown that benevolence toward women can be even more pernicious than explicit hostility because it is not clearly recognizable as a form of prejudice and thus it is more difficult to combat. Similarly, benevolence toward men predicts the perceived legitimacy of gender hierarchy. However, the effects of sexism on men’s and women’s leadership aspiration have not been studied yet. In the present study participants (N = 101) were primed with sexism (Hostile vs. Benevolent) toward their own gender. Results showed that benevolence fosters the possibility of holding a leadership role for men, but harms women from leader positions. Moreover, women consider benevolent and hostile attitudes toward women as similarly prejudiced. On the contrary, men define hostile sexism toward men as more prejudiced than benevolent attitudes., Chiara Rollero, Angela Fed., and Obsahuje seznam literatury