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22572. Variability in the karyotypes, testes and ovaries of planthoppers of the families Issidae, Caliscelidae, and Acanaloniidae (Hemiptera: Fulgoroidea)
- Creator:
- Maryańska-Nadachowska, Anna, Kuznetsova, Valentina, Gnezdilov, Vladimir, and Drosopoulos, Sakis
- Type:
- article, model:article, and TEXT
- Subject:
- Hemiptera, Fulgoroidea, Issidae, Caliscelidae, Acanaloniidae, karyotypes, holokinetic chromosomes, testes, ovaries, evolution, and systematics
- Language:
- English
- Description:
- The aim of this study was to obtain information on the karyotypes, testes and ovaries of three fulgoroid families, mainly in the Issidae but also in the Caliscelidae and Acanaloniidae. For the Issidae, the data is for 19 species belonging to 11 genera of the subtribes Issina (2 species, 1 genus), Hysteropterina (14 species, 9 genera) and Agalmatiina (3 species, 2 genera) of the tribe Issini. The male karyotypes are shown to be quite uniform across the tribe, with 2n = 26 + X in all species studied except Latilica maculipes (Melichar, 1906) with 2n = 24 + X. The modal karyotype, 2n = 26 + X, matches the most probable ancestral state in the Fulgoroidea. In the majority of cases the number of seminal follicles in males and ovarioles in females are stable within but fairly variable among the species, the modal value of the follicle number is 10 per testis. Contrary to what might be expected from other fulgoroid families, such as the Dictyopharidae and Delphacidae, the variability in these characters revealed neither regular trends nor evident correspondence with the taxonomy of Issidae. In the Caliscelidae, all species studied had testes consisting of 6 follicles each and karyotypes of 2n = 24 + X and 26 + XY, respectively, in 3 and 1 species. The only representative studied of the Acanaloniidae, Acanalonia bivittata (Say, 1825), had 2n = 24 + X and 13 follicles in its testis. The variability in all the characters investigated is discussed and compared to other fulgoroid families, primarily to the most extensively studied families, Delphacidae and Dictyopharidae.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
22573. Variability of Mammary Carcinogenesis Induction in Female Sprague-Dawley and Wistar:Han Rats: the Effect of Season and Age
- Creator:
- Kubatka, P., Eva Ahlersová, Ivan Ahlers, Bianka Bojková, Kalická, K., Eva Adámeková, Marková, M., Chamilová, M., and Čermáková, M.
- Format:
- print, bez média, and svazek
- Type:
- article, studie, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Mammary carcinogenesis, Seasons, Critical periods, Rat strains, Susceptibility, 14, and 612
- Language:
- English
- Description:
- It is important to determine and clarify the variability of mammary carcinogenesis induction in animal experimental studies particularly in connection with chemoprevention projects. The circannual seasonal rhythms of hormone levels or various parameters within the immune system may involve factors participating in mammary gland carcinogenesis. In our study, 19 experiments were conducted and all of them lasted for about 25 weeks after chemical carcinogen administration (DMBA or NMU) under standard laboratory conditions. Females of two rat strains - a medium susceptible Sprague-Dawley strain and a very low susceptible Wistar:Han were used. We observed not only the effect of seasonal changes but also the effect of age after single or repeated carcinogen administration. The seasonal dependence of mammary carcinogenesis with higher tumor incidence during long days in comparison with winter short days has been demonstrated in Sprague-Dawley rats. In experiments on the Wistar:Han strain, certain features of seasonal character were recorded, although the very low susceptibility of this strain to mammary carcinogenesis might have influenced the results. A limited period of carcinogen administration in early puberty around postnatal days 43-46 (higher susceptibility), when compared to the period after postnatal day 50, is the factor significantly increasing incidence and frequency of mammary carcinogenesis in the Sprague-Dawley strain. Our results indicate the need to consider the effect of season and age of animals at the time of carcinogen administration on rat mammary carcinogenesis induction. However, the application of the results obtained in one strain of experimental animals may only lead to misleading conclusions., P. Kubatka, E. Ahlersová, I. Ahlers, B. Bojková, K. Kalická, E. Adámeková, M. Marková, M. Chamilová, M. Čermáková., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
22574. Variability of mitochondrial respiration in relation to sepsis-induced multiple organ dysfunction
- Creator:
- Kohoutová, M., Dejmek, J., Tůma, Z., and Kuncová, J.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- sepsis, mitochondria, oxygen consumption, multiple organ dysfunction, and animal models
- Language:
- English
- Description:
- Ample experimental evidence suggests that sepsis could interfere with any mitochondrial function; however, the true role of mitochondrial dysfunction in the pathogenesis of sepsis-induced multiple organ dysfunction is still a matter of controversy. This review is primarily focused on mitochondrial oxygen consumption in various animal models of sepsis in relation to human disease and potential sources of variability in experimental results documenting decrease, increase or no change in mitochondrial respiration in various organs and species. To date, at least three possible explanations of sepsis-associated dysfunction of the mitochondrial respiratory system and consequently impaired energy production have been suggested: 1. Mitochondrial dysfunction is secondary to tissue hypoxia. 2. Mitochondria are challenged by various toxins or mediators of inflammation that impair oxygen utilization (cytopathic hypoxia). 3. Compromised mitochondrial respiration could be an active measure of survival strategy resembling stunning or hibernation. To reveal the true role of mitochondria in sepsis, sources of variability of experimental results based on animal species, models of sepsis, organs studied, or analytical approaches should be identified and minimized by the use of appropriate experimental models resembling human sepsis, wider use of larger animal species in preclinical studies, more detailed mapping of interspecies differences and organ-specific features of oxygen utilization in addition to use of complex and standardized protocols evaluating mitochondrial respiration.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
22575. Variability of peripheral pulse wave velocity in patients with diabetes mellitus type 2 during orthostatic challenge
- Creator:
- Svačinová, Jana, Hrušková, Jana, Jakubík, Juraj, Budinskaya, Ksenia, Hidegová, Simona, Fabšík, Martin, Sieglová, Helena, Kaščáková, Zuzana, Novák, Jan, and Nováková, Zuzana
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- pulse wave velocity, variability, diabetes mellitus, arterial stiffness, and orthostasis
- Language:
- English
- Description:
- Diabetes mellitus 2 (DM2) is the seventh cause of death worldwide. One of the reasons is late diagnosis of vascular damage. Pulse wave velocity (PWV) has become an independent marker of arterial stiffness and cardiovascular risk. Moreover, the previous studies have shown the importance of beat-to-beat PWV measurement due to its variability among the heart cycle. However, variability of PWV (PWVv) of the whole body hasn't been examined yet. We have studied a group of DM II and heathy volunteers, to investigate the beat-to-beat mean PWV (PWVm) and PWVv in the different body positions. PWV of left lower and upper extremities were measured in DM2 (7 m/8 f, age 68±10 years, BP 158/90±19/9 mm Hg) and healthy controls (5 m/6 f, age 23±2 years, BP 117/76±9/5 mm Hg). Volunteers were lying in the resting position and of head-up-tilt in 45° (HUT) for 6 min. PWVv was evaluated as a mean power spectrum in the frequency bands LF and HF (0.04-0.15 Hz, 0.15-0.5 Hz). Resting PWVm of upper extremity was higher in DM2. HUT increased lower extremity PWVm only in DM2. Extremities PWVm ratio was significantly lower in DM2 during HUT compared to controls. LF and HF PWVv had the same response to HUT. Resting PWVv was higher in DM2. Lower extremity PWVv increased during HUT in both groups. PWVm and PWVv in DM2 differed between extremities and were significantly influenced by postural changes due to hydrostatic pressure. Increased resting PWVm and PWVv in DM2 is a marker of increased arterial stiffness.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
22576. Variability of species of Babesia Starcovici, 1893 in three sympatric ticks (Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna) at the edge of Pannonia in the Czech Republic and Slovakia
- Creator:
- Rybářová, Markéta, Honsová, Michaela, Ivo Papoušek, and Pavel Široký
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- parazitologie, parasitology, host-specificity, piroplasms, species diagnosis, 18S rRNA, 2, and 59
- Language:
- English
- Description:
- The distribution, variability and host specificity of species of Babesia Starcovici, 1893 were studied in questing ticks collected on the northwestern edge of the Pannonian Basin in the south-easternmost part of the Czech Republic and in western Slovakia. The area is characterised by relatively natural floodplain habitats and the sympatric occurrence of three tick species possessing wide host spectra, namely Ixodes ricinus (Linnaeus), Dermacentor reticulatus (Fabricius) and Haemaphysalis concinna Koch. Analysis was carried out on 1,408 I. ricinus, 2,999 D. reticulatus and 150 H. concinna altogether, collected from 59 localities. We documented the presence of Babesia spp. not only in I. ricinus but also in H. concinna in the Czech Republic. Two isolates from I. ricinus were classified as B. venatorum Herwaldt, Cacciò, Gherlinzoni, Aspöck, Slemenda, Piccaluga, Martinelli, Edelhofer, Hollenstein, Poletti, Pampiglione, Löschenberger, Tura et Pieniazek, 2003 (formerly determined as Babesia sp. EU1), which is a zoonotic parasite and can cause human babesiosis. The rest of our amplicons were very similar to B. canis (Piana et Galli-Valerio, 1895), which is usually transmitted by D. reticulatus. Despite the huge amount of examined samples, all D. reticulatus ticks were Babesia-free. Due to this finding, we did not consider our obtained isolates to be B. canis, but other closely related species possessing a similar sequence of the studied portion of 18S rDNA. Although this genetic marker is most frequently used in PCR-based diagnostic methods of babesias, its low variability compromises its reliability in studies based only on this marker., Markéta Rybářová, Michaela Honsová, Ivo Papoušek, Pavel Široký., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
22577. Variability of vegetation of exposed pond bottoms in relation to management and environmental factors
- Creator:
- Šumberová, Kateřina, Lososová, Zdeňka, Fabšičová, Martina, and Horáková, Viera
- Type:
- article and TEXT
- Subject:
- beta-diversity, Czech Republic, fishpond, ordination, species richness, and storage pond
- Language:
- English
- Description:
- Species composition, structure and ecological characteristics of the vegetation of two pond types with different management, fishponds and storage ponds, in the Českobudějovická pánev basin (South Bohemia), were compared. A selection of 99 relevés from fishponds and 99 from storage ponds (small ponds used for the storage of marketable fish) made in 2000–2004 were analysed using direct and indirect ordination and ANOVA. The difference between storage ponds and fishponds was found to be more important than gradients correlated with temporal changes, soil moisture and mud depth. Storage ponds had a significantly higher mean number of species, bryophytes, archaeophytes and neophytes and beta-diversity. There were no significant differences in cover values, except of moss layer, which had significantly higher cover in storage ponds. Fishponds had significantly higher mean Ellenberg indicator values for light, continentality, moisture and nutrients. Oenanthe aquatica and Rumex maritimus are typical fishpond species and Amblystegium humile and Eleocharis palustris agg. typical storage pond species. The management of storage ponds is more varied and of different intensity than that of fishponds. It is assumed that management is a crucial factor determining the species richness and influencing the vegetation of these two habitats.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/
22578. Variable exponent Sobolev spaces with zero boundary values
- Creator:
- Harjulehto, Petteri
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- variable exponent, Sobolev space, and zero boundary value
- Language:
- English
- Description:
- We study different definitions of the first order variable exponent Sobolev space with zero boundary values in an open subset of Rn.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
22579. Variable expression of hypercholesterolemia in apolipoprotein E2* (Arg136 - Cys) Heterozygotes
- Creator:
- Jaroslav Hubáček, Jan Piťha, Petr Stávek, Schmitz, G., and Rudolf Poledne
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, sekvenování DNA, metabolismus lipidů, DNA sequencing, lipid metabolism, apolipoprotein E, rare mutation, PCR, 14, and 612
- Language:
- English
- Description:
- In the process of population screening for apo E gene polymorphism with the PCR and subsequent restriction analysis, we identified a female who demonstrated heterozygosity for an unusual restriction fragment caused by the loss of a CfoI restriction site. Sequence analysis of the apo E gene was performed and a carrier of the mutant allele with C - T substitution at cDNA position 3817 was identified, which caused an Arg136 - Cys change. The first-line relatives have been screened for this rare mutation with PCR and restriction analysis of PCR products. The complete lipoprotein parameters have been determined in the probands family. In the family, only one child had the same mutant allele as his mother had. The proband (7.49 mmol/l) with her siblings had hypercholesterolemia and a high body mass index (BMI 31.6 kg/m2). By contrast, her son had a normal lipid spectrum with normal BMI. We described the mutation apo E2* (Arg136 - Cys) in a family with elevated lipid levels, but there was no confirmation of the connection between this mutation and type III hyperlipoproteinemia or hyperlipoproteinemia at all. In the case of this mutation, other factors (mainly genetic) are important for the development of lipid metabolism disorders., J. A. Hubáček, J. Piťha, P. Stávek, G. Schmitz, R. Poledne., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
22580. Variable Lebesgue norm estimates for BMO functions
- Creator:
- Izuki, Mitsuo and Sawano, Yoshihiro
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- variable exponent, Morrey space, and BMO
- Language:
- English
- Description:
- In this paper, we are going to characterize the space ${\rm BMO}({\mathbb R}^n)$ through variable Lebesgue spaces and Morrey spaces. There have been many attempts to characterize the space ${\rm BMO}({\mathbb R}^n)$ by using various function spaces. For example, Ho obtained a characterization of ${\rm BMO}({\mathbb R}^n)$ with respect to rearrangement invariant spaces. However, variable Lebesgue spaces and Morrey spaces do not appear in the characterization. One of the reasons is that these spaces are not rearrangement invariant. We also obtain an analogue of the well-known John-Nirenberg inequality which can be seen as an extension to the variable Lebesgue spaces.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public