The steady-state oxygen evolution rate was previously shown to be stimulated by the disaccharide trehalose in PSII suspension. Here we showed a similar increase in the rate of oxygen evolution in PSII core complexes from spinach in solution and in proteoliposomes in the presence of trehalose. Using direct electrometrical technique, we also revealed that trehalose had no effect on the kinetics of electron transfer from Mn to redox-active-tyrosyl radical, YZ (S1 - S2 transition), while it accelerated the kinetics of electrogenic proton transport during S2 - S3 and S4 - S0 transitions of the wateroxidizing complex (WOC) induced by the first, second, and third laser flashes in dark-adapted PSII samples. These observations imply that the effect of trehalose occurrs due to its interaction with the WOC., M. D. Mamedov, E. S. Nosikova, L. A. Vitukhnovskaya, A. A. Zaspa, A. Yu. Semenov., and Obsahuje bibliografické odkazy
Photosynthetic activity of leaf disks from chlortoluron (2 µmol per plant) treated and non-treated non-transgenic and transgenic (PGF-6) tobacco plants was measured from 1 up to 21 d after treatment under greenhouse conditions. PGF-6 plants, expressing the fused rat cytochrome P4501A1/yeast reductase genes were used. PGF-6 plants were much more chlortoluron-resistant than control plants. In non-transgenic tobacco plants the electron transport flow to PQ pool was strongly inhibited 1 d after treatment with herbicide whereas it was still existing in PGF-6 plants although some reduction was observed. The quantum yield of photosystem 2 (ΦPS2) which is related to the quantum yield of whole-chain electron transfer was much more inhibited by chlortoluron than the primary PS2 photochemistry, measured by the ratio Fv/Fm. Lower PS2 activity was found for herbicide-treated non-transgenic plants up to the 9th day. Then it started to increase in both control and PGF-6 plants, but more rapidly in PGF-6 ones, and its values were near to the control level at the 21st d after chlortoluron treatment. and E. Yordanova ... [et al.].
The effect of water on the primary photosynthetic activity of purple bacterium Rhodospirillum rubrum was studied in Hexadecane-Tween-Spane (HTS)- and phospholipid (PLC)-reverse micelles. Reverse micelles offer the possibility of modulating the amount of water to which enzymes and multienzymatic complexes are exposed. Fast bacteriochlorophyll (BChl) fluorescence induction kinetics and reaction centre absorption changes at 820 nm were used as an assay for the functional transfer of bacterial cells into HTS-reverse micelles and bacterial photosynthetic complexes (BPC) into PLC-reverse micelles. Both the bacterial cells and BPC showed an increase in the rate of primary photosynthetic activity by increasing the concentration of water in the reverse micelles. The bacterial cells could be kept viable for many hours in HTS-reverse micelles in presence of 6% (v/v) water. NMR studies indicated that the photosynthetic activity was affected by the availability of water in reverse micelles. The bacterial cells in HTS or BPC in PLC reverse micelles could be used to further understand the influence of water on the organisation and function of photosynthetic complexes. and A. Srivastava, A. Darszon, R. J. Strasser.
The combined effects of water stress (WS) and low irradiance (LI) on growth, photosynthesis, osmotic adjustment, and lipid peroxidation were studied in dragon spruce (Picea asperata Mast.) seedlings grown under two water treatments (well watered, 100 % of field capacity, and water stressed, 30 % of field capacity) and two irradiances (HI, 100 % of full sunlight and LI, 15 % of full sunlight). WS reduced growth, chlorophyll (Chl) a and b contents, net photosynthetic rate, transpiration rate, stomatal conductance, and effective quantum yield of photosystem 2 (Y) but increased free proline and malondialdehyde contents. LI increased Chl contents and decreased Y, photochemical quenching (qP), and non-photochemical quenching (qN) under both water treatments. Hence the seedlings in the understory were more sensitive to drought than to LI. and Y. Yang ... [et al.].
A gradual reduction in leaf water potential (Ψleaf), net photosynthetic rate (PN), stomatal conductance, and transpiration rate was observed in two drought tolerant (C 306 and K 8027) and two susceptible (RW 893 and 899) genotypes subjected to water stress. The extent of reduction was lower in K 8027 and C 306 and higher in RW 893 and RW 899. Rewatering the plants after 5 d of stress restored PN and other gas exchange traits in all four cultivars. Water stress had no significant effect on variable to maximum fluorescence ratio (Fv/Fm) indicating that water stress had no effect on primary photochemistry of photosystem 2 (PS2). However, water stress reduced the efficiency of excitation energy transfer (F'v/F'm) and the quantum yield of electron transport (ΦPS2). The reduction was more pronounced in susceptible cultivars. Water stress had no significant effect on photochemical quenching, however, the non-photochemical quenching increased by water stress. and D. Subrahmanyam ... [et al.].
The treatment of green algae Chlorococcum lobatum with the herbicide BASTA containing phosphinothricin lead to a significant decrease in the level of peak M2 of the chlorophyll fluorescence induction curve. This agrees with the suggestion that glutamine synthetase activity affects this region of the induction curve.
Chlorophyll fluorescence kinetics was used to investigate the effect of 1,4-dithiothreitol (DTT) on the distribution of excitation energy between photosystem 1 (PS1) and photosystem 2 (PS2) in soybean leaves under high irradiance (HI). The maximum PS2 quantum yield (Fv/Fm) was hardly affected by the presence of DTT, however, photon-saturated photosynthesis was depressed distinctly. Photochemical efficiency of open PS2 reaction centres during irradiation (Fv'/Fm') was enhanced by about 30-40 % by DTT treatment, whereas photochemical quenching (qP) was depressed by about 40 % under HI. DTT treatment caused a 30 % decrease in allocation of excitation energy to PS1 under HI and a 20 % increase to PS2. An obvious shift in the balance of excitation energy distribution between photosystems was observed in DTT-treated leaves. Though high excitation pressure (1 - qP) resulted from DTT treatment, non-photochemical quenching (qN) was lower. DTT completely inhibited the formation of zeaxanthin and also distinctly depressed the state transition (qT). The shift in the balance of excitation distribution between the two photosystems induced by DTT was mainly due to the enhancement of excitation energy capture by PS2 antenna and the inhibition of state transition. It might be the shift in the balance between the two photosystems that mainly induced the depression of photosynthesis. Thus, to keep high utilization efficiency of absorbed photon energy, it is necessary to maintain the balance of excitation distribution between PS2 and PS1. and C.-D. Jiang ... [et al.].
Isoxaflutole [5-cyclopropyl-4-(2-methylsulphonyl-4-trifluromethylbenzoyl)isoxazole] is a new preemergence herbicide for broad-spectrum weed control in maize. The effect of isoxaflutole on chlorophyll (Chl) and carotenoid (Car) biosynthesis was investigated in cucumber (Cucumis sativus L.) cotyledons. Etiolated tissue was incubated with 5 mM isoxaflutole for 24 h and irradiated (60 μmol m-2 s-1). The irradiation for 3 h did not reduce Chl a, Chl b, and Car contents, but after a 28-h irradiation the contents of Chl a and Car decreased by 35 and 15 %, respectively, and the content of Chl b increased by 24 %. Increasing the concentration of isoxaflutole beyond 5 mM resulted in reduction of Chl a (71 %), Chl b (20 %), and Car (31 %) contents. Similarly, increase in irradiance from 60 to 180 μmol m-2 s-1 resulted in larger reduction of Chl and Car contents. Exogenously supplied 5-aminolevulinic acid did not reverse the isoxaflutole-inhibited Chl synthesis, whereas an exogenously supplied homogentisic acid lactone reversed the inhibition of pigment synthesis due to isoxaflutole. and S. Kushwaha, P. C. Bhowmik.