In the present study, we investigated the antioxidative potential in leaves of the chromatic (CC) versus green (GC) Amaranthus tricolor L. under moderate high-temperature stress at 45°C. Before heat stress, CC had significantly higher levels of betacyanins [about 3.2 mg g-1(FM)] than the green [1.8 mg g-1(FM) (p<0.01), while similar chlorophyll (Chl) content [about 2 mg g-1(FM)] was observed between both cultivars. After exposure to high temperature (45°C) for 6 days, betacyanins in leaves of CC were remarkably increased (about 2 times of that in control samples grown at 30°C). In contrast, betacyanins in GC significantly decreased by 56% in comparison with that of the control. Chl level in CC was higher than that in GC after heat stress for 6 days. Flavonoids and total phenolics in both cultivars were increased, but much more in CC. Significantly less H2O2 accumulation was observed in the leaves and stems of CC than in those of GC under heat stress. Interestingly, much stronger circadian oscillation in fluorescence was observed in both cultivars after treatment at 45°C, which suggested that heat stress stimulates endogenous rhythms of photosystem II (PSII). Under moderate high-temperature stress, Chl fluorescence parameters Fv/Fm (maximum quantum yield of PSII), qP (coefficient of photochemical quenching), ΦPSII (effective PSII quantum yield), and ETR (electron transport rate) exhibited a gradual decrease, NPQ (nonphotochemical quenching) showed a slight increase followed by a gradual decline, whereas Fo (minimum fluorescence of a dark-adapted leaf) increased continuously. In contrast to GC, after 120 h of high-temperature treatment, CC exhibited significantly lower Fo level, and higher levels of Fv/Fm and NPQ. It is clear that PSII in CC was more stable than that in GC. The results indicate that betacyanins are an effective antioxidant, and probably contribute greatly to the higher thermal stability of PSII and higher tolerance to heat stress. and Z. Shu ... [et al.].
A key physical property used in the description of a soil-water regime is a soil water retention curve, which shows the relationship between the water content and the water potential of the soil. Pedotransfer functions are based on the supposed dependence of the soil water content on the available soil characteristics, e.g., on the relative content of the particle size in the soil and the dry bulk density of the soil. This dependence could be extracted from the available data by various regression methods. In this paper, artificial neural networks (ANNs) and support vector machines (SVMs) were used to estimate a drying branch of a water retention curve. The paper compares the mentioned methods by estimating the water retention curves on regional scale for the Záhorská lowland in the Slovak Republic, where relatively small data set was available. The performance of the models was evaluated and compared. These computations did not fully confirm the superiority of SVMs over ANNs as is often proclaimed in the literature, because the results obtained show that in this study the ANN model performs somewhat better and is easier to handle in determining pedotransfer functions than the SVM models. Nevertheless, the results from both data-driven models are quite close, and the results show that they provide a significantly more precise outcome than a traditional multi-linear regression does., Autori sa v príspevku venujú určovaniu pedotransferových funkcií (PTF), ktoré umožňujú stanoviť body vlhkostných retenčných kriviek pôdy z ľahšie merateľných pôdnych vlastností a sú dôležitým prvkom modelovania vodného režimu pôdy. Ešte v minulej dekáde sa objavili snahy využívať na ich určenie umelé neurónové siete (UNS). Multi-layer perceptron (MLP) čiže viacvrstvový perceptrón je najčastejšie používaný model doprednej umelej neurónovej siete s kontrolovaným typom učenia. Vstupné signály prechádzajú sieťou typu MLP iba dopredným smerom, teda postupne od vrstvy k vrstve. MLP používa tri a viac vrstiev neurónov rozdelených na vstupnú, skrytú a výstupnú vrstvu s nelineárnou aktivačnou funkciou a vie rozpoznať alebo modelovať informácie, ktoré nie sú lineárne oddeliteľné alebo závislé. Novší vývoj v oblasti učiacich algoritmov poskytuje ďalšie možnosti, z ktorých sa v tomto príspevku venujeme tzv. mechanizmom podporných vektorov (Support Vector Machines - SVM). SVM využíva pri svojom kalibrovaní na riešený problém princíp tzv. štrukturálnej minimalizácie namiesto iba minimalizácie chyby - (Vapnik, 1995). Pri trénovaní siete MLP je jediným cieľom minimalizovať celkovú chybu. Pri SVM sa simultánne minimalizuje chyba aj zložitosť modelu. Použitie tohto princípu vedie zvyčajne k vyššej schopnosti generalizácie, t.j. umožneniu presnejších predpovedí pre dáta, ktoré neboli použité pri trénovaní SVM. Vhodnosť štandardnej umelej neurónovej siete, SVM a viacnásobnej lineárnej regresie sa v článku vyhodnocuje na základe údajov získaných z pôdnych vzoriek odobratých v lokalite Záhorskej nížiny. Pôvodné údaje a ich aplikáciu pri vyhodnocovaní vodného režimu pôd uvádza Skalová (2001, 2007), odkiaľ boli prevzaté vstupné dáta a to percentuálny obsah zrnitostných kategórií (I až IV podľa Kopeckého), redukovaná objemová hmotnosť (ρd) a vlhkosti pre vlkostné potenciály hw= -2.5, -56, -209, -558, -976, -3060, -15300 cm, ktoré boli stanovené laboratórne pre potreby určenia a testovania regresných závislostí. Vzhľadom na to, že pri odvodzovaní regionálnych PTF je častým prípadom nedostatok dát pre odvodenie dátovo riadených modelov, autori navrhli riešiť úlohu pomocou ansámblu MLP resp. SVM. Ansámbel dátovo riadených modelov bol vytvorený variabilným rozdelením údajov na trénovacie a validačné (validačnými údajmi sa testuje presnosť modelu vo fáze jeho tvorby, ešte sa používajú konečné testovacie dáta, ktoré neboli pri tvorbe modelu použité). Výsledky ukázali lepšie regresné schopnosti oboch dátovo riadených modelov (SVM aj MLP) voči multilineárnej regresii a o niečo lepšie výsledky boli získané z viacvrstvového perceptrónu než zo SVM., and Keďže v niektorých iných prácach mal zvyčajne vyššiu výpočtovú presnosť model založený na SVM než na UNS, autori odporúčajú pre budúci výskum preveriť vhodnosť kombinácie SVM a MLP modelov v dátovo riadenom skupinovom modeli.
Introduction: The dataset of 826 patients who were suspected of the prostate cancer was examined. The best single marker and the combination of markers which could predict the prostate cancer in very early stage of the disease were looked for. Methods: For combination of markers the logistic regression, the multilayer perceptron neural network and the k-nearest neighbour method were used. 10 models for each method were developed on the training data set and the predictive accuracy verified on the test data set. Results and conclusions: The ROCs for the models were constructed and AUCs were estimated. All three examined methods have given comparable results. The medians of estimates of AUCs were 0.775, which were larger than AUC of the best single marker.
The objective of this study was to evaluate the influence of low-dose combined oral contraception (COC) on basal and stimulated (1 μg ACTH test) levels of serum and salivary cortisol (F), cortisone and on basal serum cortisol binding globulin (CBG), adrenocorticotropic hormone (ACTH), dehydroepiadrosterone (DHEA) and calculated free cortisol in healthy young women. Three-month administration of COC resulted in 1) significant increase of basal (454.0±125.0 to 860.9±179.7 nmol/l) and ACTH-stimulated serum cortisol in 30th min (652.3±60.5 to 1374.1±240.6 nmol/l); 2) no significant change of basal (15.4±7.3 to 18.9±8.5 nmol/l) and ACTH-stimulated salivary cortisol at the 30th min (32.4±8.8 to 32.9±9.0 nmol/l); 3) no significant change of basal serum cortisone (38,8±7.68 to 45.2±24.2 nmol/l) and ACTH-stimulated cortisone at the 30th (34.8±10.9 to 47.0±35.7 nmol/l); 4) significant increase of basal ACTH (17.2±9.0 to 38.2±29.4 ng/l), CBG (991.0±161.0 to 2332.0±428.0 nmol/l), and 5) no significant change of basal DHEA (24.6±15.7 to 22.6±11.7 μmol/l) and calculated basal value for free cortisol (22.8±14.9 to 19.2±6.9nmol/l). In conclusions, higher basal and ACTH-stimulated serum cortisol were found after three-month administration of COC, while basal and stimulated salivary cortisol were not significantly affected. Therefore, salivary cortisol can be used for assessment of adrenal function in women regularly using COC., K. Šimůnková, L. Stárka, M. Hill, L. Kříž, R. Hampl, K. Vondra., and Obsahuje bibliografii a bibliografické odkazy
The erosion, transport and deposition of sediments in small valley reservoirs represent a significant impact on their operations, mainly with regard to reducing the volume of their accumulation. The aim of this study is a comparison and uncertainty analysis of two modelling concepts for assessment of soil loss and sediment transport in a small agricultural catchment, with an emphasis on estimating the off-site effects of soil erosion resulted in sedimentation of a small water reservoir. The small water reservoir (polder) of Svacenicky Creek which was built in 2012, is a part of the flood protection measures in Turá Lúka and is located in the western part of Slovakia, close to the town of Myjava. The town of Myjava in recent years has been threatened by frequent floods, which have caused heavy material losses and significantly limited the quality of life of the local residents. To estimate the amount of soil loss and sediments transported from the basin, we applied two modelling concepts based on the USLE/SDR and WaTEM/SEDEM erosion models and validated the results with the actual bathymetry of the polder. The measurements were provided by a modern Autonomous Underwater Vehicle (AUV) hydrographic instrument. From the sediment data measured and the original geodetic survey of the terrain conducted at the time of the construction of the polder, we calculated changes in the storage volume of the polder during its four years of operation. The results show that in the given area, there has been a gradual clogging of the bottom of the polder caused by water erosion. We estimate that within the four years of the acceptance run, 10,494 m3 of bottom sediments on the Svacenicky Creek polder have accumulated. It therefore follows that repeated surveying of the sedimentation is very important for the management of the water reservoir.
Let P be a discrete multidimensional probability distribution over a finite set of variables N which is only partially specified by the requirement that it has prescribed given marginals {PA;A∈\SS}, where \SS is a class of subsets of N with ⋃\SS=N. The paper deals with the problem of approximating P on the basis of those given marginals. The divergence of an approximation P^ from P is measured by the relative entropy H(P|P^). Two methods for approximating P are compared. One of them uses formerly introduced concept of {\em dependence structure simplification\/} (see Perez \cite{Per79}). The other one is based on an {\em explicit expression}, which has to be normalized. We give examples showing that neither of these two methods is universally better than the other. If one of the considered approximations P^ really has the prescribed marginals then it appears to be the distribution P with minimal possible multiinformation. A simple condition on the class \SS implying the existence of an approximation P^ with prescribed marginals is recalled. If the condition holds then both methods for approximating P give the same result.
The heterogeneity of water flow and solute transport was assessed during radioactive tracer infiltration experiment in a black clay loam soil using modified methods to estimate the effective cross section (ECS) and the degree of preferential flow (DPF). The results of field and numerical experiments showed that these parameters characterized the heterogeneity of water flow in the soils unequivocally. The ECS decreases non-linearly and the DPF increases linearly with an increase of the bypassing ratio (ratio of macropore flow rate to total flow rate). The ECS decreased and the DPF increased with depth, which suggests an increase in the heterogeneity of water flow with depth. The plot of the DPF against ECS values calculated from the tracer experiment data was consistent with the relationship obtained by the numerical simulation assuming preferential flow in the neighbourhood of three probes.
Analysis of two methods used for ascertaining stability of dynamical systems was carried out on a mathematical model of experimental stand of aerodynamic bearings. It was proofed that method of direct eigenvalue calculation gives practically the same results, which are obtained from the solution of equations of motion by means of integral Runge Kutta procedure. and Obsahuje seznam literatury
The development of knowledge on geodynamic processes is one of the most important issues in the Earth’s science. Over decades, geodetic techniques have been applied to study the geodynamics. The Global Navigation Satellite Systems (GNSS) have been reliably used for monitoring geodynamic processes. The satellite gravimetric missions such as GRACE (Gravity Recovery And Climate Experiment) and GRACE Follow-On (GRACE-FO) missions have provided numerous valuable information concerning temporal mass variations within the Earth system which can subsequently be converted to surface deformations of the Earth. The main aim of this study is to compare vertical deformations of the Earth's surface over the area of SouthEastern Poland obtained from GNSS data with the corresponding ones determined from GRACE data. The GNSS data for the period between 2008 and 2013 from 25 permanent GNSS stations operating in South-Eastern Poland and the latest release of GRACE-based Global Geopotential Models (GGMs) were used. GNSS data and GRACE-based GGMs were processed with the GAMIT/GLOBK and the IGiK‒TVGMF (Institute of Geodesy and Cartography - Temporal Variations of Gravity/Mass Functionals) packages, respectively. The results obtained indicate that monthly vertical deformations of the Earth’s surface determined using GNSS data are generally in a good agreement with the corresponding ones obtained from GRACE satellite mission data. Coefficients of correlation between these vertical deformations range from 0.60 to 0.90 and standard deviations of their differences are in the range of 2.6 - 5.7 mm., Walyeldeen Godah, Malgorzata Szelachowska, Jagat Dwipendra Ray and Jan Krynski., and Obsahuje bibliografii
Laboratory animals (mice and guinea pigs) were infected with the isolates of Coxiella burnetii (Derrick, 1939) obtained from bovine milk (M18 and M35) and the ticks Ixodes ricinus (Linnaeus, 1758) and Dermacentor marginatus (Sulzer, 1776) (Kl3 and Kl6, respectively), and with the reference strain Nine Mile. Neither mortality nor lethality occurred with the mice. Antibody response in mice infected with isolates from milk was lower (1 : 16-512) than that from ticks (1 : 32-4096). Onset of seropositivity also occurred later - on the 10th day post-infection (p.i.) for M18 and M35 in comparison with the 7th day for Kl3 and Kl6. In guinea pigs, infection manifested by fever. The fever was less evident in guinea pigs infected with isolates from milk (39.5-40.1°C) than in guinea pigs infected with isolates from ticks (39.5-40.6°C). Partially engorged females of Dermacentor reticulatus (Fabricius, 1794) were inoculated with isolates M18 and Kl3 . No differences in the multiplication of C. burnetii in haemocytes between these two isolates were ascertained.