Přijetím konceptu deskové tektoniky v 60. letech 20. století se od základu změnil náhled na původ latinskoamerické flóry. Zjistilo se, že dnešní kontinent Jižní Ameriky byl před 100 miliony lety součástí pradávného kontinentu Gondwany a její jednotné flóry. Tento poznatek vedl k interpretaci vzniku disjunktních areálů taxonů rozšířených v Jižní Americe a na ostatních částech bývalé Gondwany v důsledku rozpadu dřívějšího kontinuálního areálu výskytu. Po následném oddělení kontinentu Jižní Ameriky od Gondwany přibližně před 100 miliony lety se předpokládalo, že se zde vegetace vyvíjela izolovaně až do vytvoření Panamské šíje před 3 miliony lety. Novější výzkumy a použití tzv. molekulárních hodin však ukazují, že výměna bioty mezi Jižní Amerikou a sousedícími kontinenty v určitých obdobích ještě před uzavřením Panamské šíje přeci jen probíhala., The concept of plate tectonics accepted during the 20th century fundamentally altered general opinion on the origin of Latin American flora. South America formed a part of the ancient continent Gondwana, which had a unique flora. The separation of South America finished about 100 million years ago led to the isolation of its flora until the formation of the Isthmus of Panama about three million years ago. The current disjunctions of widely distributed tropical taxa originated after this disruption. However, recent studies based on molecular clock approaches show that the migration of biota between South America and the rest of the world occurred repeatedly even before the closure of the Isthmus., and Anna Potůčková, Daniel Stančík.
Pro odhad stáří jednotlivých vývojových linií latinskoamerické flóry se dnes využívají také tzv. molekulární hodiny. Výsledky jejich aplikace ukazují, že se řada prvků současné flóry dostala do Latinské Ameriky až po rozpadu prakontinentu Gondawana, tzn. jsou mladšího stáří než by odpovídalo době rozpadu Gondwany. Do Latinské Ameriky se mohly rozšířit dvěma různými mechanismy: migrací rostlin přes pevninu a nebo disperzí diaspor na dlouhou vzdálenost přes oceány. Důkazem migrace tropických čeledí přes kontinenty je i řada fosilních nálezů, které mohou také posloužit jako druhý nezávislý zdroj datování jejich původu., So called molecular clocks are nowadays used to estimate the age of individual lineages of Latin American flora. The results of their application show that a number of elements of current flora came to Latin America after the collapse of the ancient continent of Gondwana. This could be explained by two different mechanisms: by migration of plants across the land or by dispersion of their diasporas across the oceans. The amount of fossils proving migration of tropical families across continents is often used as the second, independent source for dating their origin., and Anna Potůčková, Daniel Stančík.
Současná diverzita tropické flóry a bioty Latinské Ameriky je výsledkem řady historických událostí, ke kterým došlo během jejího dlouhého vývoje. Výskyt stejných či příbuzných čeledí, rodů nebo druhů v oblastech a na kontinentech představujících součásti někdejšího prakontinentu Gondwana jsou výsledkem působení tří mechanismů: rozpadu souvislého areálu výskytu (vikariance) na území někdejší Gondwany, migrací rostlin přes kontinenty severní polokoule a disperzí přes oceány. Při mezikontinentálním rozšíření řady čeledí však často nehrál roli jen jeden z výše uvedených mechanismů, ale často šlo o jejich kombinace. Tím se stává studium historie různých taxonů komplikovanější. K jejímu odhalení je potřeba mít jasnou představu o stáří jejich jednotlivých vývojových linií, umožňující správnou interpretaci působení konkrétního mechanismu na jejich dnešní rozšíření a diverzitu., The tropical flora and biota of Latin America was influenced during its long history by several events that led to their current diversity and disjunct distribution. Three mechanisms have been proposed to explain the occurrence of the same or closely related families, genera and/or species among fragments of former Gondwana: vicariance, migration of plants across the continents and long distance dispersion. Recent intercontinental distribution of several taxa is commonly a result of their combination. Good knowledge of the age of principal lineages is needed for the correct interpretation of the effect of these mechanisms on the distribution and diversity of the investigated lineages., and Anna Potůčková, Daniel Stančík.
Quametopia gen. n. is established on the basis of recent phylogenetic analysis of taxa formerly assembled under the genus Mumetopia Melander, 1913 to include M. terminalis (Loew, 1863) and two new closely related Nearctic species of Anthomyzidae. The new genus is diagnosed and its phylogenetic relationships discussed. Quametopia terminalis (Loew, 1863) comb. n. is transferred from Mumetopia and redescribed based on revision of the type material (lectotypes of Anthophilina terminalis Loew, 1863 and its synonym Mumetopia nitens Melander, 1913 are designated) and other extensive material. Quametopia clintonia sp. n. and Q. amplistylus sp. n. (both from Canada, USA) are described and relationships of all three Quametopia species discussed. Immature stages of Q. terminalis and Q. clintonia sp. n. obtained by means of adult-to-adult rearing are described (1st- and 2nd-instar larvae for the first time in the family Anthomyzidae) and illustrated. Keys to adults, eggs, larvae and puparia of Quametopia species are presented. Biology (habitat and host-plant associations, life history) of Q. terminalis and Q. clintonia sp. n. are studied in detail and their ecological separation demonstrated. Distribution of all Quametopia species is reviewed. and Jindřich Roháček, Kevin N. Barber.
There is one feature common to most types of cancer - profound changes in their cellular metabolism that accommodate the high requirements for fast growth and cell division. This change brings about many advantages to the transformed cell and it is also indispensable for its survival and proliferation. This review describes the differences in metabolism between normal and cancerous cells and outlines strategies that could exploit these differences as tools for oncological treatment. and Věra Slaninová, Alena Krejčí.
Pyridoxal kinase (PLK; EC 2.7.1.35) is a key enzyme in the metabolism of vitamin B6 (VB6) in Bombyx mori. A fusion expressional vector pET-22b-BPLK-His was constructed using a sub-cloning technique, the recombinant B. mori PLK was then expressed in Escherichia coli, purified and characterized. Bioinformatics were used to deduce the protein structure and genomic organization of this enzyme. Using Ni Sepharose affinity column chromatography, the recombinant protein was purified to very high degree (approximately 90%). The recombinant PLK exhibits a high specific enzymatic activity (1800 nmol/min/mg of protein). The maximum catalytic activity of this enzyme was recorded over a narrow pH range (5.5-6.0) and Zn2+ is the most effective cation for catalysis under saturating substrate concentrations. When only triethanolamine is present as the cation, K+ is an activator of PLK. A double reciprocal plot of initial velocity suggests that the enzyme catalyses the reaction by means of a sequential catalytic mechanism. Under optimal conditions, the Km value for the substrates of ATP and pyridoxal are 57.9 ± 5.1 and 44.1 ± 3.9 µM. B. mori's genome contains a single copy of the PLK gene, which is 7.73 kb long and contains five exons and four introns, and is located on the eighth chromosome. The PLK may be a dimer with two identical subunits under native conditions, and it is hypothesized that each monomer contains eight α-helices (α1-8), nine β-strands (β1-9) and two segments of 310 helices. and Shuo-Hao HUANG, Wang MA, Ping-Ping ZHANG, Jian-Yun ZHANG, Yan-Feng XIE, Long-Quan HUANG.
The oldest butterfly fossil known, which was formed about 55 Ma in what is now Denmark, is described. The fragments of its forewing venation indicates it belongs to the Hesperiidae. Further reconstruction indicates that it fits in the Coeliadinae and is close to the extant genera Hasora and Burara. It is here described as Protocoeliades kristenseni gen. et sp. n. It is the first butterfly fossil found on a continent (Europe) where its closest relatives do not currently occur. Its position on the phylogenetic tree of the Coeliadinae and its importance in understanding the time dimension in the evolution of butterflies, and their ecological and biogeographic implications are discussed., Rienk De Jong., and Obsahuje bibliografii
In this review, we focused on the intersection between steroid metabolomics, obstetrics and steroid neurophysiology to give a comprehensive insight into the role of sex hormones and neuroactive steroids (NAS) in the mechanism controlling pregnancy sustaining. The data in the literature including our studies show that there is a complex mechanism providing synthesis of either pregnancy sust aining or parturition provoking steroids. This mechanism includes the boosting placental synthesis of CRH with approaching parturition inducing the excessive synthesis of 3β-hydroxy-5-ene steroid sulfates serving primarily as precursors for placental synthesis of progestogens, estrogens and NAS. The distribution and changing activities of placental oxidoreductases are responsible for the activation or inactivation of the aforementioned steroids, which is compartment-specific (maternal and fetal compartments) and dependent on gestational age, with a tendency to shift the production from the pregnancy-sustaining steroids to the parturition provoking ones with an increasing gestational age. The fetal and maternal livers catabolize part of the bioactive steroids and also convert some precursors to bioactive steroids. Besides the progesterone, a variety of its 5α/β-reduced metabolites may significantly influence the maintenance of human pregnancy, provide protection against excitotoxicity following acute hypoxic stress, and might also affect the pain perception in mother and fetus. and M. Hill ... [et al.].
Metabolic consequences of direct muscle trauma are insufficiently defined. Their effects on the ubiquitin-proteasome pathway (UPP) of protein degradation in human skeletal muscles are as yet unknown. Thus, we investigated whether the UPP is involved in the metabolic response evoked in directly traumatized human skeletal muscles. Biopsies were obtained from contused muscles after fractures and from normal muscles during elective implant removal (control). As estimated by western blot analyses, concentrations of free ubiquitin and ubiquitin protein conjugates were similar in extracts from injured and uninjured muscles. Ubiquitin protein ligation rates were reduced after injury (1.5±0.2 vs. 1.0±0.15 fkat/μg; p=0.04). Chymotryptic-, tryptic- and caspase-like proteasome peptidase activities (total activity minus activity in the presence of proteasome inhibitors) increased significantly after trauma (p=0.04 - 0.001). Significant increases in total chymotryptic- and caspase-like activities were attributable to proteasome activation. Our results extend the possible role of the UPP in muscle wasting to direct muscle trauma. They further suggest that the effects of direct mechanical trauma are not limited to the proteasome and imply that ubiquitin protein ligase systems are also involved. Based on the potential role of the UPP in systemic diseases, it might also be a therapeutic target to influence muscle loss in critically ill blunt trauma patients, in which large proportions of muscle are exposed to direct trauma. and Obsahuje bibliografii a bibliografické odkazy
Naše znalosti o ekologii a rozšíření řady druhů hub byly po dlouhou dobu založeny pouze na pozorování plodnic a sporulujících útvarů v terénu a na identifikaci izolovaných druhů hub. Nedávné pokroky a dostupnost metod založených na studiu DNA a jejich téměř rutinní používání v laboratořích rozšířilo naše znalosti o substrátech kolonizovaných různými druhy hub. Na druhou stranu se ukázalo, že některé druhy kolonizují stejný substrát, ale na geograficky velmi vzdálených lokalitách. Některé druhy ani nelze zařadit do jasně vymezené ekologické skupiny, mění svou ekologii v průběhu svého životního cyklu., Our information about the ecology and distribution of particular fungal species has for a long time been based only on observations of fruit bodies and sporulating structures in the field and on identification of isolated fungal strains. Recent developments in molecular methods and their routine use in mycology have revealed that some fungi may colonize different substrates than originally supposed. On the other hand, other fungi colonize their typical habitats in localities that are enormously distant from each other. Some fungi may not be easily assigned to a single ecological group, as they change various life styles during their life cycle., and Ondřej Koukol.