Confocal Stereology and Image Analysis: Methods for Estimating Geometrical Characteristics of Cells and Tissues from Three-Dimensional Confocal Images

Title:

Confocal Stereology and Image Analysis: Methods for Estimating Geometrical Characteristics of Cells and Tissues from Three-Dimensional Confocal Images

Creator:

Kubínová, L., Janáček, J., Karen, P., Radochová, B., Difato, F., and Krekule, I.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:30de7c75-0d47-4eef-9357-52a032ba4cf6
uuid:30de7c75-0d47-4eef-9357-52a032ba4cf6

Subject:

Confocal microscopy, Image analysis, Stereology, and Three dimensional reconstructions

Type:

article, model:article, and TEXT

Description:

A short review of confocal stereology and three-dimensional image analysis is presented, pointing out the achievements accomplished in this field by the Department of Biomathematics (Institute of Physiology, Prague). One of the methods of confocal stereology, the fakir method for surface area estimation, developed by this laboratory, is described. Methods for automatic measurement of geometrical characteristics of microscopical structures, based on 3-D image processing or surface triangulation, are discussed and compared with interactive stereological methods. Three-dimensional reconstruction programs and software implementation of stereological and digital methods as well as their practical applications are presented. The future trends are discussed.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2004 Volume:53 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false