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Start Over Creator Radochová, B.

1. Confocal Stereology and Image Analysis: Methods for Estimating Geometrical Characteristics of Cells and Tissues from Three-Dimensional Confocal Images

Creator:
Kubínová, L., Janáček, J., Karen, P., Radochová, B., Difato, F., and Krekule, I.
Type:
article, model:article, and TEXT
Subject:
Confocal microscopy, Image analysis, Stereology, and Three dimensional reconstructions
Language:
English
Description:
A short review of confocal stereology and three-dimensional image analysis is presented, pointing out the achievements accomplished in this field by the Department of Biomathematics (Institute of Physiology, Prague). One of the methods of confocal stereology, the fakir method for surface area estimation, developed by this laboratory, is described. Methods for automatic measurement of geometrical characteristics of microscopical structures, based on 3-D image processing or surface triangulation, are discussed and compared with interactive stereological methods. Three-dimensional reconstruction programs and software implementation of stereological and digital methods as well as their practical applications are presented. The future trends are discussed.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Excess irradiance causes early symptoms of senescence during leaf expansion in photoautotrophically in vitro grown tobacco plants

Creator:
Radochová, B. and Tichá, I.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
chlorophyll a fluorescence, leaf area, leaf dry mass, leaf ontogeny, net oxygen evolution rate, Nicotiana, and photosynthesis
Language:
Multiple languages
Description:
Photosynthetic parameters, growth, and pigment contents were determined during expansion of the fourth leaf of in vitro photoautotrophically cultured Nicotiana tabacum L. plants at three irradiances [photosynthetically active radiation (400-700 nm): low, LI 60 µmol m-2 s-1; middle, MI 180 µmol m-2 s-1; and high, HI 270 µmol m-2 s-1]. During leaf expansion, several symptoms usually accompanying leaf senescence appeared very early in HI and then in MI plants. Symptoms of senescence in developing leaves were: decreasing chlorophyll (Chl) a+b content and Chl a/b ratio, decreasing both maximum (FV/FM) and actual (ΦPS2) photochemical efficiency of photosystem 2, and increasing non-photochemical quenching. Nevertheless, net photosynthetic oxygen evolution rate (PN) did not decrease consistently with decrease in Chl content, but exhibited a typical ontogenetic course with gradual increase. PN reached its maximum before full leaf expansion and then tended to decline. Thus excess irradiance during in vitro cultivation did not cause early start of leaf senescence, but impaired photosynthetic performance and Chl content in leaves and changed their typical ontogenetic course. and B. Radochová, I. Tichá.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public