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Start Over Subject Stereology

1. Confocal Stereology and Image Analysis: Methods for Estimating Geometrical Characteristics of Cells and Tissues from Three-Dimensional Confocal Images

Creator:
Kubínová, L., Janáček, J., Karen, P., Radochová, B., Difato, F., and Krekule, I.
Type:
article, model:article, and TEXT
Subject:
Confocal microscopy, Image analysis, Stereology, and Three dimensional reconstructions
Language:
English
Description:
A short review of confocal stereology and three-dimensional image analysis is presented, pointing out the achievements accomplished in this field by the Department of Biomathematics (Institute of Physiology, Prague). One of the methods of confocal stereology, the fakir method for surface area estimation, developed by this laboratory, is described. Methods for automatic measurement of geometrical characteristics of microscopical structures, based on 3-D image processing or surface triangulation, are discussed and compared with interactive stereological methods. Three-dimensional reconstruction programs and software implementation of stereological and digital methods as well as their practical applications are presented. The future trends are discussed.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. The Effect of a Unilateral Muscle Transplantation on the Muscle Fiber Type and the MyHC Isoform Content in Unoperated Hind Limb Slow and Fast Muscles of the Inbred Lewis Rats

Creator:
Zachařová, G., Vadászová, A., Smerdu, V., Asmussen, G., and Soukup, T.
Type:
article, model:article, and TEXT
Subject:
Rat hind limb muscle, Muscle fiber phenotype, Stereology, Immunocytochemistry, Gel electrophoresis, Influence of surgery, and Muscle transplantation
Language:
English
Description:
To reveal the effect of foreign innervation and altered thyroid status on fiber type composition and the myosin heavy chain (MyHC) isoform expression in the rat slow soleus (SOL) and fast extensor digitorum longus (EDL) muscles, a method of heterochronous isotransplantation was developed. In this experimental procedure, the SOL or EDL muscles of young inbred Lewis rats are grafted either into the host EDL or SOL muscles of adult rats of the same strain with normal or experimentally altered thyroid status. To estimate the extent of fiber type transitions in the transplanted muscles, the SOL and EDL muscle from the unoperated leg and unoperated muscles from the operated leg could be legitimately used as controls, but only when the experimental procedure itself does not affect these muscles. To verify this assumption, we have compared the fiber type composition and the MyHC isoform content of unoperated contralateral SOL and EDL muscles and ipsilateral unoperated SOL muscle of experimental rats after unilateral isotransplantation into the host EDL muscle with corresponding muscles of the naive rats of the same age and strain. We provide compelling evidence that the unilateral heterochronous isotransplantation has no significant effect on the fiber type composition and the MyHC isoform content of unoperated muscles of experimental animals. Hence, these muscles can be used as controls in our grafting experiments.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public